##################################
# NO CUT ENZYMES ( 49 enzymes) #
##################################
Acc65I AccIII AgeI AocI AscI
Asp718I AviII BglI BlpI Bpu1102I
BseAI BsiWI BspEI BsrBI BsrFI
BstEII BstPI Bsu36I CelII Cfr10I
CpoI Eco81I EcoO65I FseI FspI
KpnI KspI MluI MroI NaeI
NgoMIV NheI NruI PacI PinAI
PmeI PvuI RsrII SacII SalI
SbfI SfiI SgrAI SmaI SnaBI
Sse8387I SwaI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 36 enzymes) #
###################################
AatII 2266 GACGTC GACGT^C
AclI 10335 AACGTT AA^CGTT
AhdI 228 GACCCCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApaI 5537 GGGCCC GGGCC^C
AspEI 228 GACCCCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbeI 92 GGCGCC GGCGC^C
BbrPI 8227 CACGTG CAC^GTG
BbvCI 9307 CCTCAGC CCTCAGC(-5/-2)
BspDI 1183 ATCGAT AT^CGAT
BspMI 10037 GCAGGT ACCTGC(4/8)
BssHII 206 GCGCGC G^CGCGC
Bst1107I 1445 GTATAC GTA^TAC
BstBI 7395 TTCGAA TT^CGAA
BstZ17I 1445 GTATAC GTA^TAC
ClaI 1183 ATCGAT AT^CGAT
DrdI 8675 GACAAGGAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 228 GACCCCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRV 1930 GATATC GAT^ATC
HgaI 6783 GACGC GACGC(5/10)
HpaI 764 GTTAAC GTT^AAC
KasI 92 GGCGCC G^GCGCC
NarI 92 GGCGCC GG^CGCC
NotI 185 GCGGCCGC GC^GGCCGC
NspV 7395 TTCGAA TT^CGAA
PaeR7I 8059 CTCGAG C^TCGAG
PmaCI 8227 CACGTG CAC^GTG
PmlI 8227 CACGTG CAC^GTG
PshAI 3212 GACATCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 10335 AACGTT AA^CGTT
SapI 10758 GCTCTTC GCTCTTC(1/4)
SexAI 9542 ACCTGGT A^CC[AT]GGT
SfoI 92 GGCGCC GGC^GCC
SfuI 7395 TTCGAA TT^CGAA
SphI 4626 GCATGC GCATG^C
StuI 3902 AGGCCT AGG^CCT
XhoI 8059 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AflII 1640 CTTAAG C^TTAAG
6169 CTTAAG
Alw44I 6396 GTGCAC G^TGCAC
10892 GTGCAC
Aor51HI 435 AGCGCT AGC^GCT
9280 AGCGCT
ApaLI 6396 GTGCAC G^TGCAC
10892 GTGCAC
AspI 1733 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
6513 GACTGGGTC
AvrII 96 CCTAGG C^CTAGG
8997 CCTAGG
BanI 92 GGCGCC G^G[CT][GA]CC
7712 GGTGCC
BcgI 6671 CGAGCTCTATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1434 GCAGCCAGATCG
BfrI 1640 CTTAAG C^TTAAG
6169 CTTAAG
BlnI 96 CCTAGG C^CTAGG
8997 CCTAGG
BsaWI 458 TCCGGT [AT]^CCGG[AT]
1402 ACCGGA
BspHI 6426 TCATGA T^CATGA
7975 TCATGA
BstAPI 1948 GCATTCCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
10417 GCATATTTTGC
DraIII 5017 CACAAAGTG CAC[ACGT][ACGT][ACGT]^GTG
9711 CACGCAGTG
Eco47III 435 AGCGCT AGC^GCT
9280 AGCGCT
NcoI 1134 CCATGG C^CATGG
10777 CCATGG
PflFI 1733 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
6513 GACTGGGTC
PflMI 282 CCAGAGTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
8467 CCAGCTCGTGG
PstI 7078 CTGCAG CTGCA^G
7784 CTGCAG
RcaI 6426 TCATGA T^CATGA
7975 TCATGA
SacI 6672 GAGCTC GAGCT^C
8261 GAGCTC
ScaI 2783 AGTACT AGT^ACT
5186 AGTACT
Tth111I 1733 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
6513 GACTGGGTC
Van91I 282 CCAGAGTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
8467 CCAGCTCGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 149 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AflIII AluI AlwI AlwNI
ApoI AseI AsnI Asp700I AspHI
AvaI AvaII BalI BamHI BanII
BbsI BbvI BciVI BclI BcnI
BfaI BglII BmyI BpmI BpuAI
BsaAI BsaBI BsaHI BsaI BsaJI
BseRI BsgI BsiEI BsiHKAI BsiYI
BslI BsmAI BsmBI BsmFI BsmI
BsoBI Bsp1286I Bsp1407I BspLU11I BsrDI
BsrGI BsrI BssKI BssSI BstF5I
BstNI BstUI BstXI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraI
DraII DsaI EaeI EagI EarI
EclXI Eco52I EcoNI EcoO109I EcoRI
EcoRII EcoT14I EcoT22I FauI FbaI
Fnu4HI FokI HaeII HaeIII HapII
HhaI Hin1I HinP1I HincII HindII
HindIII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MamI
MboI MboII MfeI MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MunI MvaI MvnI MwoI
NciI NdeI NdeII NlaIII NlaIV
NsiI NspI PleI PpuMI PshBI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI SpeI SspBI
SspI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XbaI XcmI XhoII XmnI