##################################
# NO CUT ENZYMES ( 88 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AflII AgeI Alw44I AlwNI AocI
Aor51HI ApaLI AscI Asp718I AspI
AviII BalI BamHI BbeI BbvCI
BfrI BglI BlpI Bpu1102I BsaHI
BseAI BsiWI BsmBI BspEI BsrBI
BsrDI BssHII BssSI BstBI BstEII
BstPI Bsu36I CelII CpoI DrdI
EagI EclXI Eco47III Eco52I Eco81I
EcoO65I FseI FspI HgaI Hin1I
HpaI KasI KpnI KspI MluI
MluNI MroI MscI NarI NotI
NspV PacI PflFI PflMI PinAI
PmeI Psp1406I RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI Sse8387I StuI SwaI Tth111I
Van91I XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AccII 106 CGCG CG^CG
AhdI 3971 GACTATCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApaI 237 GGGCCC GGGCC^C
AspEI 3971 GACTATCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbrPI 114 CACGTG CAC^GTG
BbsI 2411 GAAGAC GAAGAC(2/6)
BcgI 195 GCAACGTCGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 2825 TGATCA T^GATCA
BpuAI 2411 GAAGAC GAAGAC(2/6)
BsaWI 3967 ACCGGA [AT]^CCGG[AT]
BsgI 1291 CTGCAC GTGCAG(16/14)
BsiEI 102 CGATCG CG[GA][CT]^CG
BspDI 2722 ATCGAT AT^CGAT
BspHI 3936 TCATGA T^CATGA
BsrFI 399 GCCGGC [GA]^CCGG[CT]
BstAPI 3244 GCAGTGAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 106 CGCG CG^CG
BstXI 3554 CCAAGATTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CfoI 324 GCGC GCG^C
Cfr10I 399 GCCGGC [GA]^CCGG[CT]
ClaI 2722 ATCGAT AT^CGAT
DraIII 4695 CACCCTGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 3971 GACTATCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 786 CCTGACTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI 2825 TGATCA T^GATCA
HaeII 323 AGCGCC [GA]GCGC^[CT]
HhaI 324 GCGC GCG^C
HinP1I 324 GCGC G^CGC
MvnI 106 CGCG CG^CG
NaeI 399 GCCGGC GCC^GGC
NgoMIV 399 GCCGGC G^CCGGC
NheI 2097 GCTAGC G^CTAGC
NruI 105 TCGCGA TCG^CGA
PaeR7I 3049 CTCGAG C^TCGAG
PmaCI 114 CACGTG CAC^GTG
PmlI 114 CACGTG CAC^GTG
PshAI 4043 GACAGTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 1852 CTGCAG CTGCA^G
PvuI 102 CGATCG CGAT^CG
RcaI 3936 TCATGA T^CATGA
SpeI 3880 ACTAGT A^CTAGT
XbaI 1820 TCTAGA T^CTAGA
XcmI 157 CCATCTCGGAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 3049 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AlwI 1968 GATCC GGATC(4/5)
2785 GATCC
AspHI 4430 GTGCTC G[AT]GC[AT]^C
5096 GAGCAC
AvaI 337 CTCGGG C^[CT]CG[GA]G
3049 CTCGAG
BanI 396 GGTGCC G^G[CT][GA]CC
4693 GGCACC
BciVI 6780 GGATAC GTATCC(6/5)
5738 GTATCC
BcnI 87 CCGGG CC^[GC]GG
533 CCCGG
BglII 4929 AGATCT A^GATCT
5277 AGATCT
BsaAI 114 CACGTG [CT]AC^GT[GA]
3798 TACGTG
BsaI 3727 GAGACC GGTCTC(1/5)
869 GGTCTC
BsiHKAI 4430 GTGCTC G[AT]GC[AT]^C
5096 GAGCAC
BsmI 509 GAATGC GAATGC(1/-1)
3249 GAATGC
BsoBI 337 CTCGGG C^[CT]CG[GA]G
3049 CTCGAG
Bsp1407I 4092 TGTACA T^GTACA
6173 TGTACA
BsrGI 4092 TGTACA T^GTACA
6173 TGTACA
Bst1107I 2609 GTATAC GTA^TAC
4471 GTATAC
BstYI 4929 AGATCT [GA]^GATC[CT]
5277 AGATCT
BstZ17I 2609 GTATAC GTA^TAC
4471 GTATAC
DraII 5192 AGGACCC [GA]G^G[ACGT]CC[CT]
5564 AGGTCCT
DsaI 1388 CCATGG C^C[GA][CT]GG
3291 CCATGG
EaeI 401 CGGCCA [CT]^GGCC[GA]
500 TGGCCG
EcoO109I 5192 AGGACCC [GA]G^G[ACGT]CC[CT]
5564 AGGTCCT
EcoRV 5498 GATATC GAT^ATC
6521 GATATC
EcoT22I 511 ATGCAT ATGCA^T
6668 ATGCAT
MflI 4929 AGATCT [GA]^GATC[CT]
5277 AGATCT
MspA1I 1923 CAGCTG C[AC]G^C[GT]G
6762 CAGCTG
NciI 87 CCGGG CC^[GC]GG
533 CCCGG
NcoI 1388 CCATGG C^CATGG
3291 CCATGG
NdeI 2039 CATATG CA^TATG
2829 CATATG
NsiI 511 ATGCAT ATGCA^T
6668 ATGCAT
PpuMI 5192 AGGACCC [GA]G^G[AT]CC[CT]
5564 AGGTCCT
PvuII 1923 CAGCTG CAG^CTG
6762 CAGCTG
SphI 1103 GCATGC GCATG^C
4967 GCATGC
SspBI 4092 TGTACA T^GTACA
6173 TGTACA
XhoII 4929 AGATCT [GA]^GATC[CT]
5277 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 92 enzymes) #
#########################################
AccI AciI AcsI AfaI AflIII
AluI ApoI AseI AsnI Asp700I
AvaII AvrII BanII BbvI BfaI
BlnI BmyI BpmI BsaBI BsaJI
BseRI BsiYI BslI BsmAI BsmFI
Bsp1286I BspLU11I BspMI BsrI BssKI
BstF5I BstNI Cac8I Cfr13I DdeI
DpnII DraI EarI EcoRI EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HincII HindII HindIII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MamI MboI MboII
MfeI MnlI MseI MslI MspI
MunI MvaI MwoI NdeII NlaIII
NlaIV NspI PleI PshBI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SspI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XmnI