##################################
# NO CUT ENZYMES ( 38 enzymes) #
##################################
AatII AclI AscI AseI AsnI
AvrII BbrPI BlnI BsiWI BspLU11I
BssHII CpoI EagI EclXI Eco52I
EcoRV FseI MfeI MunI NaeI
NdeI NgoMIV NotI NruI PacI
PaeR7I PmaCI PmeI PmlI PshBI
Psp1406I PvuI RsrII ScaI SfiI
SnaBI SwaI XhoI
###################################
# ONE CUT ENZYMES ( 28 enzymes) #
###################################
Acc65I 4345 GGTACC G^GTACC
AccIII 993 TCCGGA T^CCGGA
AflII 457 CTTAAG C^TTAAG
AgeI 2062 ACCGGT A^CCGGT
AhdI 8221 GACCGCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp718I 4345 GGTACC G^GTACC
AspEI 8221 GACCGCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BfrI 457 CTTAAG C^TTAAG
BseAI 993 TCCGGA T^CCGGA
BspEI 993 TCCGGA T^CCGGA
Bst1107I 827 GTATAC GTA^TAC
BstZ17I 827 GTATAC GTA^TAC
DrdI 1924 GACAAGGAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 8221 GACCGCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoT22I 1946 ATGCAT ATGCA^T
HindIII 4220 AAGCTT A^AGCTT
HpaI 535 GTTAAC GTT^AAC
KpnI 4345 GGTACC GGTAC^C
MluI 1253 ACGCGT A^CGCGT
MroI 993 TCCGGA T^CCGGA
NheI 2541 GCTAGC G^CTAGC
NsiI 1946 ATGCAT ATGCA^T
PinAI 2062 ACCGGT A^CCGGT
SalI 814 GTCGAC G^TCGAC
SbfI 3496 CCTGCAGG CCTGCA^GG
SgrAI 2061 CACCGGTG C[GA]^CCGG[CT]G
SpeI 11014 ACTAGT A^CTAGT
Sse8387I 3496 CCTGCAGG CCTGCA^GG
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
Alw44I 1563 GTGCAC G^TGCAC
5239 GTGCAC
ApaLI 1563 GTGCAC G^TGCAC
5239 GTGCAC
AviII 4697 TGCGCA TGC^GCA
6437 TGCGCA
BbeI 4403 GGCGCC GGCGC^C
10297 GGCGCC
BcgI 3231 CGATGTGCATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1565 GCACAGCCTTCG
BciVI 758 GGATAC GTATCC(6/5)
9646 GGATAC
BclI 3416 TGATCA T^GATCA
8600 TGATCA
BglI 5017 GCCATCTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
6538 GCCCAAGTGGC
BsaAI 9517 TACGTG [CT]AC^GT[GA]
11035 TACGTG
BsiEI 8220 CGACCG CG[GA][CT]^CG
9462 CGACCG
BstBI 4216 TTCGAA TT^CGAA
5200 TTCGAA
DraI 1057 TTTAAA TTT^AAA
2533 TTTAAA
FbaI 3416 TGATCA T^GATCA
8600 TGATCA
FspI 4697 TGCGCA TGC^GCA
6437 TGCGCA
KasI 4403 GGCGCC G^GCGCC
10297 GGCGCC
NarI 4403 GGCGCC GG^CGCC
10297 GGCGCC
NspV 4216 TTCGAA TT^CGAA
5200 TTCGAA
PshAI 2253 GACCAATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
7905 GACCGCAGTC
SfoI 4403 GGCGCC GGC^GCC
10297 GGCGCC
SfuI 4216 TTCGAA TT^CGAA
5200 TTCGAA
SmaI 3242 CCCGGG CCC^GGG
5736 CCCGGG
SphI 3237 GCATGC GCATG^C
9440 GCATGC
XbaI 4723 TCTAGA T^CTAGA
8421 TCTAGA
XmaCI 3242 CCCGGG C^CCGGG
5736 CCCGGG
XmaI 3242 CCCGGG C^CCGGG
5736 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 167 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AflIII AluI AlwI AlwNI
AocI Aor51HI ApaI ApoI Asp700I
AspHI AspI AvaI AvaII BalI
BamHI BanI BanII BbsI BbvCI
BbvI BcnI BfaI BglII BlpI
BmyI BpmI Bpu1102I BpuAI BsaBI
BsaHI BsaI BsaJI BsaWI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmBI BsmFI BsmI BsoBI Bsp1286I
Bsp1407I BspDI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BsrI BssKI
BssSI BstAPI BstEII BstF5I BstNI
BstPI BstUI BstXI BstYI Bsu36I
Cac8I CelII CfoI Cfr10I Cfr13I
ClaI DdeI DpnII DraII DraIII
DsaI EaeI EarI Eco47III Eco81I
EcoNI EcoO109I EcoO65I EcoRI EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HincII HindII HinfI HpaII
HphI ItaI Ksp632I KspI MaeI
MaeII MaeIII MamI MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NcoI NdeII NlaIII
NlaIV NspI PflFI PflMI PleI
PpuMI PstI PvuII RcaI RsaI
SacI SacII SapI Sau3AI Sau96I
ScrFI SexAI SfaNI SfcI SmlI
SspBI SspI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI Tth111I TthHB8I Van91I XcmI
XhoII XmnI