##################################
# NO CUT ENZYMES ( 80 enzymes) #
##################################
Acc65I AccIII AclI AgeI Aor51HI
ApaI AscI AseI AsnI Asp718I
AspI AvrII BamHI BbeI BbrPI
BglI BglII BlnI BlpI Bpu1102I
BseAI BsiWI Bsp1407I BspEI BspLU11I
BsrGI BssSI Bst1107I BstBI BstZ17I
CelII CpoI Eco47III EcoRV EcoT22I
FseI HaeII HpaI KasI KpnI
KspI MfeI MroI MunI NarI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
RsrII SacI SacII SbfI ScaI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI Sse8387I SspBI SspI
SwaI Tth111I XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 32 enzymes) #
###################################
AatII 1687 GACGTC GACGT^C
AccI 1597 GTCGAC GT^[AC][GT]AC
AhdI 500 GACATTCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 500 GACATTCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 165 TGCGCA TGC^GCA
BbvCI 5458 GCTGAGG CCTCAGC(-5/-2)
BclI 3640 TGATCA T^GATCA
BcnI 5074 CCGGG CC^[GC]GG
BsaWI 1810 ACCGGA [AT]^CCGG[AT]
BsmBI 3495 GAGACG CGTCTC(1/5)
BspDI 2178 ATCGAT AT^CGAT
BsrBI 1791 GAGCGG CCGCTC(-3/-3)
BssHII 134 GCGCGC G^CGCGC
ClaI 2178 ATCGAT AT^CGAT
DraI 5488 TTTAAA TTT^AAA
DrdI 4419 GACCAAGATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 500 GACATTCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 465 GAATTC G^AATTC
FbaI 3640 TGATCA T^GATCA
FspI 165 TGCGCA TGC^GCA
HincII 1597 GTCGAC GT[CT]^[GA]AC
HindII 1597 GTCGAC GT[CT]^[GA]AC
HindIII 222 AAGCTT A^AGCTT
MluI 5005 ACGCGT A^CGCGT
NciI 5074 CCGGG CC^[GC]GG
NdeI 308 CATATG CA^TATG
NheI 95 GCTAGC G^CTAGC
PvuI 531 CGATCG CGAT^CG
SalI 1597 GTCGAC G^TCGAC
SapI 5506 GCTCTTC GCTCTTC(1/4)
SphI 3391 GCATGC GCATG^C
XbaI 2294 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AflIII 1272 ACGTGT A^C[GA][CT]GT
5005 ACGCGT
Alw44I 2494 GTGCAC G^TGCAC
3569 GTGCAC
AocI 2406 CCTCAGG CC^T[ACGT]AGG
2953 CCTCAGG
ApaLI 2494 GTGCAC G^TGCAC
3569 GTGCAC
BanI 3177 GGCACC G^G[CT][GA]CC
4023 GGTGCC
BsaAI 1271 TACGTG [CT]AC^GT[GA]
4717 TACGTG
BsaBI 324 GATCCTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
830 GATGGAAATC
BsmI 2397 GAATGC GAATGC(1/-1)
4082 GAATGC
BspHI 332 TCATGA T^CATGA
4129 TCATGA
BstAPI 3418 GCAGCGAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3604 GCACACCCTGC
Bsu36I 2406 CCTCAGG CC^T[ACGT]AGG
2953 CCTCAGG
DraIII 3502 CACAGTGTG CAC[ACGT][ACGT][ACGT]^GTG
3556 CACGCTGTG
EagI 209 CGGCCG C^GGCCG
2079 CGGCCG
EclXI 209 CGGCCG C^GGCCG
2079 CGGCCG
Eco52I 209 CGGCCG C^GGCCG
2079 CGGCCG
Eco81I 2406 CCTCAGG CC^T[ACGT]AGG
2953 CCTCAGG
EcoNI 1044 CCTGCTGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3137 CCTCCGGCAGG
MamI 324 GATCCTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
830 GATGGAAATC
PflMI 2709 CCAGTGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3286 CCAATACCTGG
RcaI 332 TCATGA T^CATGA
4129 TCATGA
SexAI 1709 ACCTGGT A^CC[AT]GGT
2032 ACCAGGT
Van91I 2709 CCAGTGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3286 CCAATACCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 124 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflII AluI AlwI AlwNI ApoI
Asp700I AspHI AvaI AvaII BalI
BanII BbsI BbvI BcgI BciVI
BfaI BfrI BmyI BpmI BpuAI
BsaHI BsaI BsaJI BseRI BsgI
BsiEI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrDI
BsrFI BsrI BssKI BstEII BstF5I
BstNI BstPI BstUI BstXI BstYI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
EcoO109I EcoO65I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NaeI
NcoI NdeII NgoMIV NlaIII NlaIV
NspI PleI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XcmI XhoII XmnI