##################################
# NO CUT ENZYMES ( 56 enzymes) #
##################################
Acc65I AflII AgeI Alw44I Aor51HI
ApaLI AscI Asp718I AviII BamHI
BbeI BbrPI BciVI BfrI BglI
BsaI BsiWI BsrBI BssHII Bst1107I
BstEII BstPI BstZ17I CpoI DrdI
Eco47III EcoO65I EcoRV FseI FspI
HpaI KasI KpnI KspI MfeI
MunI NarI NheI NruI PacI
PaeR7I PinAI PmaCI PmeI PmlI
RsrII SacI SacII SbfI SexAI
SfiI SfoI SpeI Sse8387I XbaI
XhoI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
AatII 814 GACGTC GACGT^C
AccII 5791 CGCG CG^CG
AclI 5808 AACGTT AA^CGTT
AhdI 4261 GACTTTATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApaI 1779 GGGCCC GGGCC^C
AspEI 4261 GACTTTATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 4015 CCTAGG C^CTAGG
BbvCI 826 CCTCAGC CCTCAGC(-5/-2)
BclI 3214 TGATCA T^GATCA
BglII 4421 AGATCT A^GATCT
BlnI 4015 CCTAGG C^CTAGG
BlpI 4315 GCTTAGC GC^T[ACGT]AGC
Bpu1102I 4315 GCTTAGC GC^T[ACGT]AGC
BsaAI 2686 TACGTA [CT]AC^GT[GA]
BsaBI 3353 GATTCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmBI 851 CGTCTC CGTCTC(1/5)
BspDI 4547 ATCGAT AT^CGAT
BstBI 2109 TTCGAA TT^CGAA
BstUI 5791 CGCG CG^CG
BstXI 2410 CCAGCCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 4315 GCTTAGC GC^T[ACGT]AGC
ClaI 4547 ATCGAT AT^CGAT
EagI 1582 CGGCCG C^GGCCG
Eam1105I 4261 GACTTTATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 1582 CGGCCG C^GGCCG
Eco52I 1582 CGGCCG C^GGCCG
EcoNI 1477 CCTACCTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 5183 GAATTC G^AATTC
FbaI 3214 TGATCA T^GATCA
MamI 3353 GATTCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluI 5790 ACGCGT A^CGCGT
MvnI 5791 CGCG CG^CG
NcoI 1650 CCATGG C^CATGG
NdeI 3393 CATATG CA^TATG
NotI 1581 GCGGCCGC GC^GGCCGC
NspV 2109 TTCGAA TT^CGAA
Psp1406I 5808 AACGTT AA^CGTT
PstI 710 CTGCAG CTGCA^G
PvuI 4670 CGATCG CGAT^CG
SalI 817 GTCGAC G^TCGAC
SfuI 2109 TTCGAA TT^CGAA
SgrAI 1586 CGCCGGTG C[GA]^CCGG[CT]G
SmaI 1166 CCCGGG CCC^GGG
SnaBI 2686 TACGTA TAC^GTA
SphI 5506 GCATGC GCATG^C
StuI 2581 AGGCCT AGG^CCT
SwaI 4551 ATTTAAAT ATTT^AAAT
XmaCI 1166 CCCGGG C^CCGGG
XmaI 1166 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AccI 817 GTCGAC GT^[AC][GT]AC
3805 GTAGAC
AccIII 657 TCCGGA T^CCGGA
3195 TCCGGA
AseI 3955 ATTAAT AT^TAAT
4798 ATTAAT
AsnI 3955 ATTAAT AT^TAAT
4798 ATTAAT
AspI 416 GACCGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
3585 GACATGGTC
AvaI 1166 CCCGGG C^[CT]CG[GA]G
1373 CTCGGG
BanII 683 GAGCCC G[GA]GC[CT]^C
1779 GGGCCC
BcgI 5252 CGACTTTCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1613 GCAGGGGCTTCG
BsaWI 657 TCCGGA [AT]^CCGG[AT]
3195 TCCGGA
BseAI 657 TCCGGA T^CCGGA
3195 TCCGGA
BsiEI 1582 CGGCCG CG[GA][CT]^CG
4670 CGATCG
BsoBI 1166 CCCGGG C^[CT]CG[GA]G
1373 CTCGGG
BspEI 657 TCCGGA T^CCGGA
3195 TCCGGA
BspHI 283 TCATGA T^CATGA
4974 TCATGA
BspLU11I 3639 ACATGT A^CATGT
5118 ACATGT
BssSI 2309 CACGAG CACGAG(-5/-1)
3943 CTCGTG
DraIII 185 CACTTTGTG CAC[ACGT][ACGT][ACGT]^GTG
3615 CACTTAGTG
DsaI 1650 CCATGG C^C[GA][CT]GG
2703 CCACGG
HaeII 1706 AGCGCC [GA]GCGC^[CT]
3434 AGCGCC
MroI 657 TCCGGA T^CCGGA
3195 TCCGGA
NaeI 378 GCCGGC GCC^GGC
4311 GCCGGC
NgoMIV 378 GCCGGC G^CCGGC
4311 GCCGGC
PflFI 416 GACCGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
3585 GACATGGTC
PshAI 776 GACCGGCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
2423 GACAACAGTC
PshBI 3955 ATTAAT AT^TAAT
4798 ATTAAT
PvuII 524 CAGCTG CAG^CTG
2415 CAGCTG
RcaI 283 TCATGA T^CATGA
4974 TCATGA
ScaI 273 AGTACT AGT^ACT
5931 AGTACT
Tth111I 416 GACCGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
3585 GACATGGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 124 enzymes) #
#########################################
AciI AcsI AcyI AfaI AflIII
AluI AlwI AlwNI AocI ApoI
Asp700I AspHI AvaII BalI BanI
BbsI BbvI BcnI BfaI BmyI
BpmI BpuAI BsaHI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI Bsp1286I Bsp1407I BspMI
BsrDI BsrFI BsrGI BsrI BssKI
BstAPI BstF5I BstNI BstYI Bsu36I
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraI DraII EaeI EarI
Eco81I EcoO109I EcoRII EcoT14I EcoT22I
FauI Fnu4HI FokI HaeIII HapII
HgaI HhaI Hin1I HinP1I HincII
HindII HindIII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV NsiI NspI PflMI PleI
PpuMI RsaI SapI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI SspBI
SspI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XcmI XhoII XmnI