################################## # NO CUT ENZYMES ( 56 enzymes) # ################################## Acc65I AflII AgeI Alw44I Aor51HI ApaLI AscI Asp718I AviII BamHI BbeI BbrPI BciVI BfrI BglI BsaI BsiWI BsrBI BssHII Bst1107I BstEII BstPI BstZ17I CpoI DrdI Eco47III EcoO65I EcoRV FseI FspI HpaI KasI KpnI KspI MfeI MunI NarI NheI NruI PacI PaeR7I PinAI PmaCI PmeI PmlI RsrII SacI SacII SbfI SexAI SfiI SfoI SpeI Sse8387I XbaI XhoI ################################### # ONE CUT ENZYMES ( 49 enzymes) # ################################### AatII 814 GACGTC GACGT^C AccII 5791 CGCG CG^CG AclI 5808 AACGTT AA^CGTT AhdI 4261 GACTTTATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC ApaI 1779 GGGCCC GGGCC^C AspEI 4261 GACTTTATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AvrII 4015 CCTAGG C^CTAGG BbvCI 826 CCTCAGC CCTCAGC(-5/-2) BclI 3214 TGATCA T^GATCA BglII 4421 AGATCT A^GATCT BlnI 4015 CCTAGG C^CTAGG BlpI 4315 GCTTAGC GC^T[ACGT]AGC Bpu1102I 4315 GCTTAGC GC^T[ACGT]AGC BsaAI 2686 TACGTA [CT]AC^GT[GA] BsaBI 3353 GATTCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsmBI 851 CGTCTC CGTCTC(1/5) BspDI 4547 ATCGAT AT^CGAT BstBI 2109 TTCGAA TT^CGAA BstUI 5791 CGCG CG^CG BstXI 2410 CCAGCCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CelII 4315 GCTTAGC GC^T[ACGT]AGC ClaI 4547 ATCGAT AT^CGAT EagI 1582 CGGCCG C^GGCCG Eam1105I 4261 GACTTTATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EclXI 1582 CGGCCG C^GGCCG Eco52I 1582 CGGCCG C^GGCCG EcoNI 1477 CCTACCTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRI 5183 GAATTC G^AATTC FbaI 3214 TGATCA T^GATCA MamI 3353 GATTCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MluI 5790 ACGCGT A^CGCGT MvnI 5791 CGCG CG^CG NcoI 1650 CCATGG C^CATGG NdeI 3393 CATATG CA^TATG NotI 1581 GCGGCCGC GC^GGCCGC NspV 2109 TTCGAA TT^CGAA Psp1406I 5808 AACGTT AA^CGTT PstI 710 CTGCAG CTGCA^G PvuI 4670 CGATCG CGAT^CG SalI 817 GTCGAC G^TCGAC SfuI 2109 TTCGAA TT^CGAA SgrAI 1586 CGCCGGTG C[GA]^CCGG[CT]G SmaI 1166 CCCGGG CCC^GGG SnaBI 2686 TACGTA TAC^GTA SphI 5506 GCATGC GCATG^C StuI 2581 AGGCCT AGG^CCT SwaI 4551 ATTTAAAT ATTT^AAAT XmaCI 1166 CCCGGG C^CCGGG XmaI 1166 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 29 enzymes) # ################################### AccI 817 GTCGAC GT^[AC][GT]AC 3805 GTAGAC AccIII 657 TCCGGA T^CCGGA 3195 TCCGGA AseI 3955 ATTAAT AT^TAAT 4798 ATTAAT AsnI 3955 ATTAAT AT^TAAT 4798 ATTAAT AspI 416 GACCGAGTC GAC[ACGT]^[ACGT][ACGT]GTC 3585 GACATGGTC AvaI 1166 CCCGGG C^[CT]CG[GA]G 1373 CTCGGG BanII 683 GAGCCC G[GA]GC[CT]^C 1779 GGGCCC BcgI 5252 CGACTTTCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 1613 GCAGGGGCTTCG BsaWI 657 TCCGGA [AT]^CCGG[AT] 3195 TCCGGA BseAI 657 TCCGGA T^CCGGA 3195 TCCGGA BsiEI 1582 CGGCCG CG[GA][CT]^CG 4670 CGATCG BsoBI 1166 CCCGGG C^[CT]CG[GA]G 1373 CTCGGG BspEI 657 TCCGGA T^CCGGA 3195 TCCGGA BspHI 283 TCATGA T^CATGA 4974 TCATGA BspLU11I 3639 ACATGT A^CATGT 5118 ACATGT BssSI 2309 CACGAG CACGAG(-5/-1) 3943 CTCGTG DraIII 185 CACTTTGTG CAC[ACGT][ACGT][ACGT]^GTG 3615 CACTTAGTG DsaI 1650 CCATGG C^C[GA][CT]GG 2703 CCACGG HaeII 1706 AGCGCC [GA]GCGC^[CT] 3434 AGCGCC MroI 657 TCCGGA T^CCGGA 3195 TCCGGA NaeI 378 GCCGGC GCC^GGC 4311 GCCGGC NgoMIV 378 GCCGGC G^CCGGC 4311 GCCGGC PflFI 416 GACCGAGTC GAC[ACGT]^[ACGT][ACGT]GTC 3585 GACATGGTC PshAI 776 GACCGGCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 2423 GACAACAGTC PshBI 3955 ATTAAT AT^TAAT 4798 ATTAAT PvuII 524 CAGCTG CAG^CTG 2415 CAGCTG RcaI 283 TCATGA T^CATGA 4974 TCATGA ScaI 273 AGTACT AGT^ACT 5931 AGTACT Tth111I 416 GACCGAGTC GAC[ACGT]^[ACGT][ACGT]GTC 3585 GACATGGTC ######################################### # 3 OR MORE CUT ENZYMES ( 124 enzymes) # ######################################### AciI AcsI AcyI AfaI AflIII AluI AlwI AlwNI AocI ApoI Asp700I AspHI AvaII BalI BanI BbsI BbvI BcnI BfaI BmyI BpmI BpuAI BsaHI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI Bsp1286I Bsp1407I BspMI BsrDI BsrFI BsrGI BsrI BssKI BstAPI BstF5I BstNI BstYI Bsu36I Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraI DraII EaeI EarI Eco81I EcoO109I EcoRII EcoT14I EcoT22I FauI Fnu4HI FokI HaeIII HapII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV NsiI NspI PflMI PleI PpuMI RsaI SapI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SspBI SspI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI XhoII XmnI