##################################
# NO CUT ENZYMES ( 58 enzymes) #
##################################
AatII Acc65I AccIII AclI AgeI
Aor51HI AscI Asp718I AspI AvrII
BamHI BbrPI BbvCI BlnI BsaAI
BseAI BsiWI BsmBI BsmI BspDI
BspEI BssHII Bst1107I BstEII BstPI
BstZ17I ClaI DraIII DrdI Eco47III
EcoO65I EcoRI EcoRV FseI KpnI
MluI MroI NotI NruI PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PshAI Psp1406I PvuI
SalI SapI SexAI SfiI SnaBI
Tth111I Van91I XhoI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
AflII 1616 CTTAAG C^TTAAG
AhdI 2184 GACCCTCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AseI 3674 ATTAAT AT^TAAT
AsnI 3674 ATTAAT AT^TAAT
AspEI 2184 GACCCTCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 90 TGCGCA TGC^GCA
BcgI 1354 GCAGCCACTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 3155 TGATCA T^GATCA
BfrI 1616 CTTAAG C^TTAAG
BssSI 1688 CACGAG CACGAG(-5/-1)
BstAPI 3653 GCAGCTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 1362 TTCGAA TT^CGAA
BstXI 777 CCACACGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CpoI 980 CGGACCG CG^G[AT]CCG
Eam1105I 2184 GACCCTCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoT14I 2555 CCATGG C^C[AT][AT]GG
FbaI 3155 TGATCA T^GATCA
FspI 90 TGCGCA TGC^GCA
HincII 2811 GTTAAC GT[CT]^[GA]AC
HindII 2811 GTTAAC GT[CT]^[GA]AC
HindIII 3987 AAGCTT A^AGCTT
HpaI 2811 GTTAAC GTT^AAC
MfeI 3297 CAATTG C^AATTG
MunI 3297 CAATTG C^AATTG
NcoI 2555 CCATGG C^CATGG
NdeI 4104 CATATG CA^TATG
NheI 899 GCTAGC G^CTAGC
NspV 1362 TTCGAA TT^CGAA
PshBI 3674 ATTAAT AT^TAAT
RsrII 980 CGGACCG CG^G[AT]CCG
SbfI 1817 CCTGCAGG CCTGCA^GG
ScaI 2052 AGTACT AGT^ACT
SfuI 1362 TTCGAA TT^CGAA
SgrAI 1547 CACCGGCG C[GA]^CCGG[CT]G
SpeI 5206 ACTAGT A^CTAGT
Sse8387I 1817 CCTGCAGG CCTGCA^GG
StyI 2555 CCATGG C^C[AT][AT]GG
XbaI 3537 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 39 enzymes) #
###################################
AccI 4776 GTCTAC GT^[AC][GT]AC
5439 GTCTAC
Alw44I 1071 GTGCAC G^TGCAC
5268 GTGCAC
AocI 936 CCTGAGG CC^T[ACGT]AGG
2364 CCTCAGG
ApaLI 1071 GTGCAC G^TGCAC
5268 GTGCAC
BbeI 425 GGCGCC GGCGC^C
932 GGCGCC
BciVI 2228 GGATAC GTATCC(6/5)
262 GTATCC
BglI 365 GCCCGGGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
387 GCCAGCACGGC
BglII 2576 AGATCT A^GATCT
4490 AGATCT
BlpI 903 GCTGAGC GC^T[ACGT]AGC
1941 GCTCAGC
BpmI 582 CTCCAG CTGGAG(16/14)
2351 CTCCAG
Bpu1102I 903 GCTGAGC GC^T[ACGT]AGC
1941 GCTCAGC
BsaBI 203 GATGGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3033 GATGAACATC
BsaWI 528 TCCGGT [AT]^CCGG[AT]
1645 ACCGGA
Bsp1407I 1246 TGTACA T^GTACA
5014 TGTACA
BspHI 3680 TCATGA T^CATGA
4793 TCATGA
BspLU11I 2987 ACATGT A^CATGT
3440 ACATGT
BsrBI 799 CCGCTC CCGCTC(-3/-3)
596 GAGCGG
BsrGI 1246 TGTACA T^GTACA
5014 TGTACA
Bsu36I 936 CCTGAGG CC^T[ACGT]AGG
2364 CCTCAGG
CelII 903 GCTGAGC GC^T[ACGT]AGC
1941 GCTCAGC
Eco81I 936 CCTGAGG CC^T[ACGT]AGG
2364 CCTCAGG
EcoNI 1662 CCTGTGGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1752 CCTGTGGCAGG
EcoT22I 3670 ATGCAT ATGCA^T
4342 ATGCAT
KasI 425 GGCGCC G^GCGCC
932 GGCGCC
MamI 203 GATGGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3033 GATGAACATC
NaeI 305 GCCGGC GCC^GGC
908 GCCGGC
NarI 425 GGCGCC GG^CGCC
932 GGCGCC
NgoMIV 305 GCCGGC G^CCGGC
908 GCCGGC
NsiI 3670 ATGCAT ATGCA^T
4342 ATGCAT
PpuMI 316 AGGTCCC [GA]G^G[AT]CC[CT]
724 AGGACCT
PvuII 1062 CAGCTG CAG^CTG
3654 CAGCTG
RcaI 3680 TCATGA T^CATGA
4793 TCATGA
SacI 2123 GAGCTC GAGCT^C
2273 GAGCTC
SfoI 425 GGCGCC GGC^GCC
932 GGCGCC
SphI 851 GCATGC GCATG^C
2952 GCATGC
SspBI 1246 TGTACA T^GTACA
5014 TGTACA
StuI 1374 AGGCCT AGG^CCT
5523 AGGCCT
SwaI 37 ATTTAAAT ATTT^AAAT
2754 ATTTAAAT
XcmI 774 CCACCACACGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
4698 CCAACTAGGCTTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 123 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflIII AluI AlwI AlwNI ApaI
ApoI Asp700I AspHI AvaI AvaII
BalI BanI BanII BbsI BbvI
BcnI BfaI BmyI BpuAI BsaHI
BsaI BsaJI BseRI BsgI BsiEI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrDI BsrFI
BsrI BssKI BstF5I BstNI BstUI
BstYI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraI DraII DsaI
EaeI EagI EarI EclXI Eco52I
EcoO109I EcoRII FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI Ksp632I KspI MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV NspI PleI
PstI RsaI SacII Sau3AI Sau96I
ScrFI SfaNI SfcI SmaI SmlI
SspI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII
XmaCI XmaI XmnI