##################################
# NO CUT ENZYMES ( 77 enzymes) #
##################################
Acc65I AccI AclI AflII AgeI
Alw44I Aor51HI ApaLI AscI AseI
AsnI Asp700I Asp718I AviII BbeI
BcgI BclI BfrI BsaBI BsiWI
BsmBI BspDI BspLU11I BsrFI BssHII
Bst1107I BstBI BstZ17I Cfr10I ClaI
CpoI DrdI EagI EclXI Eco47III
Eco52I EcoRI EcoT22I FbaI FseI
FspI HindIII HpaI KasI KpnI
KspI MamI MluI NaeI NarI
NgoMIV NotI NruI NsiI NspV
PacI PaeR7I PinAI PmeI PshBI
Psp1406I PvuI RsrII SacII SalI
ScaI SexAI SfiI SfoI SfuI
SgrAI SnaBI SwaI TaqI TthHB8I
XhoI XmnI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AatII 684 GACGTC GACGT^C
AccIII 5321 TCCGGA T^CCGGA
AcsI 3454 AAATTC [GA]^AATT[CT]
AcyI 684 GACGTC G[GA]^CG[CT]C
AhdI 1300 GACACTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApoI 3454 AAATTC [GA]^AATT[CT]
AspEI 1300 GACACTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI 5006 GGATCC G^GATCC
BbrPI 4029 CACGTG CAC^GTG
BbsI 3771 GTCTTC GAAGAC(2/6)
BbvCI 737 CCTCAGC CCTCAGC(-5/-2)
BciVI 4241 GTATCC GTATCC(6/5)
BglII 1411 AGATCT A^GATCT
BpuAI 3771 GTCTTC GAAGAC(2/6)
BsaHI 684 GACGTC G[GA]^CG[CT]C
BsaWI 5321 TCCGGA [AT]^CCGG[AT]
BseAI 5321 TCCGGA T^CCGGA
BsiEI 443 CGGTCG CG[GA][CT]^CG
BsmI 4974 GAATGC GAATGC(1/-1)
Bsp1407I 1710 TGTACA T^GTACA
BspEI 5321 TCCGGA T^CCGGA
BspHI 4297 TCATGA T^CATGA
BsrGI 1710 TGTACA T^GTACA
BstEII 3304 GGTTACC G^GT[ACGT]ACC
BstPI 3304 GGTTACC G^GT[ACGT]ACC
DraI 5499 TTTAAA TTT^AAA
Eam1105I 1300 GACACTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 3304 GGTTACC G^GT[ACGT]ACC
EcoRV 4650 GATATC GAT^ATC
HgaI 1461 GCGTC GACGC(5/10)
Hin1I 684 GACGTC G[GA]^CG[CT]C
MfeI 492 CAATTG C^AATTG
MroI 5321 TCCGGA T^CCGGA
MunI 492 CAATTG C^AATTG
PmaCI 4029 CACGTG CAC^GTG
PmlI 4029 CACGTG CAC^GTG
PshAI 680 GACTGACGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 4297 TCATGA T^CATGA
SmlI 3494 CTTGAG C^T[CT][GA]AG
SpeI 994 ACTAGT A^CTAGT
SspBI 1710 TGTACA T^GTACA
SspI 3951 AATATT AAT^ATT
XbaI 884 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AvaI 2106 CCCGGG C^[CT]CG[GA]G
5436 CCCGGG
AvrII 4789 CCTAGG C^CTAGG
5605 CCTAGG
BlnI 4789 CCTAGG C^CTAGG
5605 CCTAGG
BsaI 188 GAGACC GGTCTC(1/5)
217 GGTCTC
BsoBI 2106 CCCGGG C^[CT]CG[GA]G
5436 CCCGGG
NdeI 1768 CATATG CA^TATG
2141 CATATG
NheI 660 GCTAGC G^CTAGC
5390 GCTAGC
PflMI 627 CCACAGGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2628 CCAGGGGCTGG
SacI 2577 GAGCTC GAGCT^C
4613 GAGCTC
SapI 2523 GCTCTTC GCTCTTC(1/4)
4595 GCTCTTC
SbfI 2431 CCTGCAGG CCTGCA^GG
3258 CCTGCAGG
SmaI 2106 CCCGGG CCC^GGG
5436 CCCGGG
Sse8387I 2431 CCTGCAGG CCTGCA^GG
3258 CCTGCAGG
StuI 3460 AGGCCT AGG^CCT
4376 AGGCCT
Van91I 627 CCACAGGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2628 CCAGGGGCTGG
XmaCI 2106 CCCGGG C^CCGGG
5436 CCCGGG
XmaI 2106 CCCGGG C^CCGGG
5436 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 121 enzymes) #
#########################################
AccII AciI AfaI AflIII AluI
AlwI AlwNI AocI ApaI AspHI
AspI AvaII BalI BanI BanII
BbvI BcnI BfaI BglI BlpI
BmyI BpmI Bpu1102I BsaAI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I BspMI BsrBI
BsrDI BsrI BssKI BssSI BstAPI
BstF5I BstNI BstUI BstXI BstYI
Bsu36I Cac8I CelII CfoI Cfr13I
DdeI DpnII DraII DraIII DsaI
EaeI EarI Eco81I EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HhaI HinP1I
HincII HindII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NcoI
NdeII NlaIII NlaIV NspI PflFI
PleI PpuMI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SphI StyI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI Tth111I XcmI
XhoII