################################## # NO CUT ENZYMES ( 77 enzymes) # ################################## Acc65I AccI AclI AflII AgeI Alw44I Aor51HI ApaLI AscI AseI AsnI Asp700I Asp718I AviII BbeI BcgI BclI BfrI BsaBI BsiWI BsmBI BspDI BspLU11I BsrFI BssHII Bst1107I BstBI BstZ17I Cfr10I ClaI CpoI DrdI EagI EclXI Eco47III Eco52I EcoRI EcoT22I FbaI FseI FspI HindIII HpaI KasI KpnI KspI MamI MluI NaeI NarI NgoMIV NotI NruI NsiI NspV PacI PaeR7I PinAI PmeI PshBI Psp1406I PvuI RsrII SacII SalI ScaI SexAI SfiI SfoI SfuI SgrAI SnaBI SwaI TaqI TthHB8I XhoI XmnI ################################### # ONE CUT ENZYMES ( 43 enzymes) # ################################### AatII 684 GACGTC GACGT^C AccIII 5321 TCCGGA T^CCGGA AcsI 3454 AAATTC [GA]^AATT[CT] AcyI 684 GACGTC G[GA]^CG[CT]C AhdI 1300 GACACTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC ApoI 3454 AAATTC [GA]^AATT[CT] AspEI 1300 GACACTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BamHI 5006 GGATCC G^GATCC BbrPI 4029 CACGTG CAC^GTG BbsI 3771 GTCTTC GAAGAC(2/6) BbvCI 737 CCTCAGC CCTCAGC(-5/-2) BciVI 4241 GTATCC GTATCC(6/5) BglII 1411 AGATCT A^GATCT BpuAI 3771 GTCTTC GAAGAC(2/6) BsaHI 684 GACGTC G[GA]^CG[CT]C BsaWI 5321 TCCGGA [AT]^CCGG[AT] BseAI 5321 TCCGGA T^CCGGA BsiEI 443 CGGTCG CG[GA][CT]^CG BsmI 4974 GAATGC GAATGC(1/-1) Bsp1407I 1710 TGTACA T^GTACA BspEI 5321 TCCGGA T^CCGGA BspHI 4297 TCATGA T^CATGA BsrGI 1710 TGTACA T^GTACA BstEII 3304 GGTTACC G^GT[ACGT]ACC BstPI 3304 GGTTACC G^GT[ACGT]ACC DraI 5499 TTTAAA TTT^AAA Eam1105I 1300 GACACTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoO65I 3304 GGTTACC G^GT[ACGT]ACC EcoRV 4650 GATATC GAT^ATC HgaI 1461 GCGTC GACGC(5/10) Hin1I 684 GACGTC G[GA]^CG[CT]C MfeI 492 CAATTG C^AATTG MroI 5321 TCCGGA T^CCGGA MunI 492 CAATTG C^AATTG PmaCI 4029 CACGTG CAC^GTG PmlI 4029 CACGTG CAC^GTG PshAI 680 GACTGACGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC RcaI 4297 TCATGA T^CATGA SmlI 3494 CTTGAG C^T[CT][GA]AG SpeI 994 ACTAGT A^CTAGT SspBI 1710 TGTACA T^GTACA SspI 3951 AATATT AAT^ATT XbaI 884 TCTAGA T^CTAGA ################################### # TWO CUT ENZYMES ( 17 enzymes) # ################################### AvaI 2106 CCCGGG C^[CT]CG[GA]G 5436 CCCGGG AvrII 4789 CCTAGG C^CTAGG 5605 CCTAGG BlnI 4789 CCTAGG C^CTAGG 5605 CCTAGG BsaI 188 GAGACC GGTCTC(1/5) 217 GGTCTC BsoBI 2106 CCCGGG C^[CT]CG[GA]G 5436 CCCGGG NdeI 1768 CATATG CA^TATG 2141 CATATG NheI 660 GCTAGC G^CTAGC 5390 GCTAGC PflMI 627 CCACAGGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2628 CCAGGGGCTGG SacI 2577 GAGCTC GAGCT^C 4613 GAGCTC SapI 2523 GCTCTTC GCTCTTC(1/4) 4595 GCTCTTC SbfI 2431 CCTGCAGG CCTGCA^GG 3258 CCTGCAGG SmaI 2106 CCCGGG CCC^GGG 5436 CCCGGG Sse8387I 2431 CCTGCAGG CCTGCA^GG 3258 CCTGCAGG StuI 3460 AGGCCT AGG^CCT 4376 AGGCCT Van91I 627 CCACAGGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2628 CCAGGGGCTGG XmaCI 2106 CCCGGG C^CCGGG 5436 CCCGGG XmaI 2106 CCCGGG C^CCGGG 5436 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 121 enzymes) # ######################################### AccII AciI AfaI AflIII AluI AlwI AlwNI AocI ApaI AspHI AspI AvaII BalI BanI BanII BbvI BcnI BfaI BglI BlpI BmyI BpmI Bpu1102I BsaAI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BspMI BsrBI BsrDI BsrI BssKI BssSI BstAPI BstF5I BstNI BstUI BstXI BstYI Bsu36I Cac8I CelII CfoI Cfr13I DdeI DpnII DraII DraIII DsaI EaeI EarI Eco81I EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NcoI NdeII NlaIII NlaIV NspI PflFI PleI PpuMI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SphI StyI TfiI Tru9I TseI Tsp45I Tsp509I TspRI Tth111I XcmI XhoII