##################################
# NO CUT ENZYMES ( 66 enzymes) #
##################################
Acc65I AclI AflII AgeI AhdI
Alw44I Aor51HI ApaLI AscI AseI
AsnI Asp718I AspEI AviII BciVI
BclI BfrI BsaBI BsiWI Bsp1407I
BspDI BspLU11I BsrGI BssHII Bst1107I
BstAPI BstBI BstZ17I ClaI CpoI
DraIII DrdI Eam1105I Eco47III EcoRI
EcoRV FbaI FseI FspI HpaI
KpnI MamI MfeI MluI MunI
NheI NotI NruI NspV PacI
PinAI PmeI PshBI Psp1406I PvuI
RsrII SalI SbfI SexAI SfiI
SfuI SgrAI SnaBI Sse8387I SspBI
SwaI
###################################
# ONE CUT ENZYMES ( 27 enzymes) #
###################################
AatII 3770 GACGTC GACGT^C
Asp700I 3787 GAATAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 4831 GACTACGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 5637 CCTAGG C^CTAGG
BglII 3849 AGATCT A^GATCT
BlnI 5637 CCTAGG C^CTAGG
BspHI 1042 TCATGA T^CATGA
BsrBI 5301 CCGCTC CCGCTC(-3/-3)
DraI 301 TTTAAA TTT^AAA
EcoNI 3483 CCTGCCCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I 1731 ATGCAT ATGCA^T
KspI 51 CCGCGG CCGC^GG
NcoI 1910 CCATGG C^CATGG
NsiI 1731 ATGCAT ATGCA^T
PflFI 4831 GACTACGTC GAC[ACGT]^[ACGT][ACGT]GTC
RcaI 1042 TCATGA T^CATGA
SacI 1106 GAGCTC GAGCT^C
SacII 51 CCGCGG CCGC^GG
ScaI 3461 AGTACT AGT^ACT
SmaI 2843 CCCGGG CCC^GGG
SpeI 5807 ACTAGT A^CTAGT
SspI 4861 AATATT AAT^ATT
Tth111I 4831 GACTACGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 858 TCTAGA T^CTAGA
XmaCI 2843 CCCGGG C^CCGGG
XmaI 2843 CCCGGG C^CCGGG
XmnI 3787 GAATAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AccI 5047 GTAGAC GT^[AC][GT]AC
5632 GTAGAC
AflIII 690 ACGTGT A^C[GA][CT]GT
5114 ACACGT
BbeI 2240 GGCGCC GGCGC^C
2298 GGCGCC
BglI 50 GCCGCGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2732 GCCAACATGGC
BssSI 128 CACGAG CACGAG(-5/-1)
623 CACGAG
EagI 235 CGGCCG C^GGCCG
426 CGGCCG
EclXI 235 CGGCCG C^GGCCG
426 CGGCCG
Eco52I 235 CGGCCG C^GGCCG
426 CGGCCG
HincII 1385 GTCAAC GT[CT]^[GA]AC
1796 GTTGAC
HindII 1385 GTCAAC GT[CT]^[GA]AC
1796 GTTGAC
HindIII 1934 AAGCTT A^AGCTT
3048 AAGCTT
KasI 2240 GGCGCC G^GCGCC
2298 GGCGCC
NarI 2240 GGCGCC GG^CGCC
2298 GGCGCC
NdeI 1259 CATATG CA^TATG
1289 CATATG
PaeR7I 76 CTCGAG C^TCGAG
580 CTCGAG
PflMI 1285 CCAGCATATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4839 CCACGCCCTGG
SapI 1551 GAAGAGC GCTCTTC(1/4)
4783 GAAGAGC
SfoI 2240 GGCGCC GGC^GCC
2298 GGCGCC
Van91I 1285 CCAGCATATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4839 CCACGCCCTGG
XhoI 76 CTCGAG C^TCGAG
580 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 145 enzymes) #
#########################################
AccII AccIII AciI AcsI AcyI
AfaI AluI AlwI AlwNI AocI
ApaI ApoI AspHI AvaI AvaII
BalI BamHI BanI BanII BbrPI
BbsI BbvCI BbvI BcgI BcnI
BfaI BlpI BmyI BpmI Bpu1102I
BpuAI BsaAI BsaHI BsaI BsaJI
BsaWI BseAI BseRI BsgI BsiEI
BsiHKAI BsiYI BslI BsmAI BsmBI
BsmFI BsmI BsoBI Bsp1286I BspEI
BspMI BsrDI BsrFI BsrI BssKI
BstEII BstF5I BstNI BstPI BstUI
BstXI BstYI Bsu36I Cac8I CelII
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI EarI Eco81I
EcoO109I EcoO65I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MroI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NaeI NciI NdeII NgoMIV
NlaIII NlaIV NspI PleI PmaCI
PmlI PpuMI PshAI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SphI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XcmI XhoII