##################################
# NO CUT ENZYMES ( 59 enzymes) #
##################################
AatII AgeI ApaI AscI AspI
AviII BbrPI BclI BglI BsaAI
BsiEI BsiWI BspDI BssHII BssSI
Bst1107I BstBI BstZ17I ClaI CpoI
EagI EclXI Eco52I EcoRV FbaI
FseI FspI HpaI KspI MfeI
MluI MunI NdeI NheI NotI
NruI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PvuI
RsrII SacII SalI SbfI SexAI
SfiI SfuI SgrAI SnaBI Sse8387I
SwaI Tth111I XcmI XhoI
###################################
# ONE CUT ENZYMES ( 57 enzymes) #
###################################
Acc65I 3115 GGTACC G^GTACC
AccIII 5595 TCCGGA T^CCGGA
AclI 3181 AACGTT AA^CGTT
AflIII 1094 ACATGT A^C[GA][CT]GT
AhdI 152 GACTGAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 2497 GTGCAC G^TGCAC
AlwNI 4932 CAGCCTCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 60 AGCGCT AGC^GCT
ApaLI 2497 GTGCAC G^TGCAC
Asp718I 3115 GGTACC G^GTACC
AspEI 152 GACTGAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 5963 CCCGGG C^[CT]CG[GA]G
BamHI 421 GGATCC G^GATCC
BbeI 5762 GGCGCC GGCGC^C
BbsI 3145 GTCTTC GAAGAC(2/6)
BcgI 5945 GCAGGAGAATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 5373 GGATAC GTATCC(6/5)
BlpI 1130 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1130 GCTGAGC GC^T[ACGT]AGC
BpuAI 3145 GTCTTC GAAGAC(2/6)
BsaWI 5595 TCCGGA [AT]^CCGG[AT]
BseAI 5595 TCCGGA T^CCGGA
BsoBI 5963 CCCGGG C^[CT]CG[GA]G
BspEI 5595 TCCGGA T^CCGGA
BspLU11I 1094 ACATGT A^CATGT
BsrFI 4172 GCCGGC [GA]^CCGG[CT]
BstAPI 4931 GCAGCCTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 1787 GGTCACC G^GT[ACGT]ACC
BstPI 1787 GGTCACC G^GT[ACGT]ACC
CelII 1130 GCTGAGC GC^T[ACGT]AGC
Cfr10I 4172 GCCGGC [GA]^CCGG[CT]
DrdI 69 GACAGTGGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 152 GACTGAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 60 AGCGCT AGC^GCT
EcoO65I 1787 GGTCACC G^GT[ACGT]ACC
EcoT22I 1276 ATGCAT ATGCA^T
KasI 5762 GGCGCC G^GCGCC
KpnI 3115 GGTACC GGTAC^C
MroI 5595 TCCGGA T^CCGGA
NaeI 4172 GCCGGC GCC^GGC
NarI 5762 GGCGCC GG^CGCC
NcoI 5708 CCATGG C^CATGG
NgoMIV 4172 GCCGGC G^CCGGC
NsiI 1276 ATGCAT ATGCA^T
PflMI 3752 CCACCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 4874 GACGGGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 3181 AACGTT AA^CGTT
PstI 4929 CTGCAG CTGCA^G
SacI 5823 GAGCTC GAGCT^C
ScaI 3550 AGTACT AGT^ACT
SfoI 5762 GGCGCC GGC^GCC
SmaI 5963 CCCGGG CCC^GGG
SpeI 3106 ACTAGT A^CTAGT
Van91I 3752 CCACCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 3005 TCTAGA T^CTAGA
XmaCI 5963 CCCGGG C^CCGGG
XmaI 5963 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 16 enzymes) #
###################################
AccI 563 GTCTAC GT^[AC][GT]AC
3621 GTAGAC
AflII 455 CTTAAG C^TTAAG
3134 CTTAAG
AvrII 3353 CCTAGG C^CTAGG
3602 CCTAGG
BanII 387 GAGCCC G[GA]GC[CT]^C
5823 GAGCTC
BfrI 455 CTTAAG C^TTAAG
3134 CTTAAG
BglII 1312 AGATCT A^GATCT
4228 AGATCT
BlnI 3353 CCTAGG C^CTAGG
3602 CCTAGG
BsaBI 1307 GATTCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2372 GATACACATC
BsmBI 4872 GAGACG CGTCTC(1/5)
5038 GAGACG
DraIII 2500 CACTGAGTG CAC[ACGT][ACGT][ACGT]^GTG
2987 CACATTGTG
HaeII 60 AGCGCT [GA]GCGC^[CT]
5762 GGCGCC
HincII 49 GTCAAC GT[CT]^[GA]AC
4645 GTTGAC
HindII 49 GTCAAC GT[CT]^[GA]AC
4645 GTTGAC
MamI 1307 GATTCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2372 GATACACATC
TaqI 662 TCGA T^CGA
5078 TCGA
TthHB8I 662 TCGA T^CGA
5078 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 126 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI AocI ApoI AseI
AsnI Asp700I AspHI AvaII BalI
BanI BbvCI BbvI BcnI BfaI
BmyI BpmI BsaHI BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI Bsp1286I Bsp1407I
BspHI BspMI BsrBI BsrDI BsrGI
BsrI BssKI BstF5I BstNI BstUI
BstXI BstYI Bsu36I Cac8I CfoI
Cfr13I DdeI DpnII DraI DraII
DsaI EaeI EarI Eco81I EcoNI
EcoO109I EcoRI EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HgaI
HhaI Hin1I HinP1I HindIII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV NspI
PleI PpuMI PshBI PvuII RcaI
RsaI SapI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI SphI SspBI
SspI StuI StyI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI XhoII
XmnI