##################################
# NO CUT ENZYMES ( 125 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AflIII AlwNI Aor51HI ApaI AscI
AseI AsnI Asp700I Asp718I AspI
AvaI AviII AvrII BalI BamHI
BbrPI BbsI BbvCI BciVI BfrI
BglI BlnI BlpI Bpu1102I BpuAI
BsaBI BsaI BseAI BsiEI BsiWI
BsoBI Bsp1407I BspDI BspEI BspLU11I
BsrGI BssHII Bst1107I BstAPI BstBI
BstXI BstZ17I CelII ClaI CpoI
DraII DraIII DrdI DsaI EaeI
EagI EclXI Eco47III Eco52I EcoNI
EcoO109I EcoRI EcoRV EcoT14I FseI
FspI HincII HindII HindIII HpaI
KpnI KspI MamI MfeI MluI
MluNI MroI MscI MunI NaeI
NcoI NdeI NgoMIV NheI NotI
NruI NspI NspV PaeR7I PflFI
PflMI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PvuI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfiI SfuI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI StyI SwaI Tth111I
Van91I XhoI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 60 enzymes) #
###################################
AccI 365 GTAGAC GT^[AC][GT]AC
AccII 202 CGCG CG^CG
AciI 513 GCGG CCGC(-3/-1)
AcsI 1276 AAATTT [GA]^AATT[CT]
AgeI 91 ACCGGT A^CCGGT
AhdI 368 GACCCCCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 215 GTGCAC G^TGCAC
AocI 381 CCTGAGG CC^T[ACGT]AGG
ApaLI 215 GTGCAC G^TGCAC
ApoI 1276 AAATTT [GA]^AATT[CT]
AspEI 368 GACCCCCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 215 GTGCAC G[AT]GC[AT]^C
BanI 991 GGCGCC G^G[CT][GA]CC
BanII 467 GGGCTC G[GA]GC[CT]^C
BbeI 991 GGCGCC GGCGC^C
BcgI 161 GCAGTATCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 305 TGATCA T^GATCA
BcnI 372 CCCGG CC^[GC]GG
BglII 566 AGATCT A^GATCT
BsaAI 218 CACGTA [CT]AC^GT[GA]
BseRI 205 GAGGAG GAGGAG(10/8)
BsgI 808 CTGCAC GTGCAG(16/14)
BsiHKAI 215 GTGCAC G[AT]GC[AT]^C
BsmBI 1144 GAGACG CGTCTC(1/5)
BsmI 533 GCATTC GAATGC(1/-1)
BspHI 939 TCATGA T^CATGA
BsrBI 511 GAGCGG CCGCTC(-3/-3)
BsrDI 820 GCAATG GCAATG(2/0)
BssSI 1308 CTCGTG CACGAG(-5/-1)
BstUI 202 CGCG CG^CG
Bsu36I 381 CCTGAGG CC^T[ACGT]AGG
DraI 1262 TTTAAA TTT^AAA
Eam1105I 368 GACCCCCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 322 GAAGAG CTCTTC(1/4)
Eco81I 381 CCTGAGG CC^T[ACGT]AGG
FauI 513 GCGGG CCCGC(4/6)
FbaI 305 TGATCA T^GATCA
HaeII 991 GGCGCC [GA]GCGC^[CT]
KasI 991 GGCGCC G^GCGCC
Ksp632I 322 GAAGAG CTCTTC(1/4)
MaeII 219 ACGT A^CGT
MslI 90 CACCGGTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 356 CAGCTG C[AC]G^C[GT]G
MvnI 202 CGCG CG^CG
NarI 991 GGCGCC GG^CGCC
NciI 372 CCCGG CC^[GC]GG
PacI 1319 TTAATTAA TTAAT^TAA
PinAI 91 ACCGGT A^CCGGT
PleI 586 GAGTC GAGTC(4/5)
PstI 4 CTGCAG CTGCA^G
PvuII 356 CAGCTG CAG^CTG
RcaI 939 TCATGA T^CATGA
SfcI 4 CTGCAG C^T[GA][CT]AG
SfoI 991 GGCGCC GGC^GCC
SgrAI 90 CACCGGTG C[GA]^CCGG[CT]G
StuI 755 AGGCCT AGG^CCT
TaqI 80 TCGA T^CGA
TthHB8I 80 TCGA T^CGA
XbaI 750 TCTAGA T^CTAGA
XcmI 1097 CCAGCATTAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AcyI 983 GGCGTC G[GA]^CG[CT]C
991 GGCGCC
BmyI 215 GTGCAC G[AGT]GC[ACT]^C
467 GGGCTC
BpmI 887 CTCCAG CTGGAG(16/14)
736 CTGGAG
BsaHI 983 GGCGTC G[GA]^CG[CT]C
991 GGCGCC
BsaJI 371 CCCCGG C^C[ACGT][ACGT]GG
1139 CCTCGG
BsmAI 1144 GAGAC GTCTC(1/5)
831 GTCTC
Bsp1286I 215 GTGCAC G[AGT]GC[ACT]^C
467 GGGCTC
BspMI 652 GCAGGT ACCTGC(4/8)
1071 GCAGGT
BsrFI 91 ACCGGT [GA]^CCGG[CT]
617 ACCGGC
BstEII 375 GGTCACC G^GT[ACGT]ACC
1074 GGTTACC
BstPI 375 GGTCACC G^GT[ACGT]ACC
1074 GGTTACC
Cfr10I 91 ACCGGT [GA]^CCGG[CT]
617 ACCGGC
EcoO65I 375 GGTCACC G^GT[ACGT]ACC
1074 GGTTACC
EcoT22I 142 ATGCAT ATGCA^T
917 ATGCAT
HgaI 846 GACGC GACGC(5/10)
984 GCGTC
Hin1I 983 GGCGTC G[GA]^CG[CT]C
991 GGCGCC
NsiI 142 ATGCAT ATGCA^T
917 ATGCAT
#########################################
# 3 OR MORE CUT ENZYMES ( 56 enzymes) #
#########################################
AfaI AluI AlwI AvaII BbvI
BfaI BsaWI BsiYI BslI BsmFI
BsrI BssKI BstF5I BstNI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
EcoRII Fnu4HI FokI HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI MaeI MaeIII MboI MboII
MflI MnlI MseI MspI MvaI
MwoI NdeII NlaIII NlaIV RsaI
Sau3AI Sau96I ScrFI SfaNI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
XhoII