##################################
# NO CUT ENZYMES ( 74 enzymes) #
##################################
Acc65I AccIII AclI AgeI AhdI
Aor51HI AscI Asp718I AspEI AviII
AvrII BbeI BbrPI BlnI BlpI
Bpu1102I BsaAI BsaWI BseAI BsiWI
BspDI BspEI BssHII BssSI BstBI
BstEII BstPI CelII ClaI CpoI
Eam1105I Eco47III EcoNI EcoO65I EcoRI
FseI FspI HaeII HgaI KasI
KpnI KspI MfeI MluI MroI
MunI NaeI NarI NgoMIV NotI
NruI NspV PacI PaeR7I PinAI
PmaCI PmlI Psp1406I PvuI RsrII
SacII SalI SbfI ScaI SexAI
SfiI SfoI SfuI SnaBI SpeI
Sse8387I SwaI XcmI XhoI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
AatII 1684 GACGTC GACGT^C
AccII 62 CGCG CG^CG
AcyI 1684 GACGTC G[GA]^CG[CT]C
AflII 2745 CTTAAG C^TTAAG
ApaI 69 GGGCCC GGGCC^C
AspI 3323 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BcgI 4875 CGACACATTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 2745 CTTAAG C^TTAAG
BglI 41 GCCCCCGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaBI 543 GATGTTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 1684 GACGTC G[GA]^CG[CT]C
BsiEI 54 CGGCCG CG[GA][CT]^CG
BsmBI 3321 GAGACG CGTCTC(1/5)
BsmI 1489 GCATTC GAATGC(1/-1)
BspMI 3545 ACCTGC ACCTGC(4/8)
BsrFI 99 ACCGGC [GA]^CCGG[CT]
Bst1107I 4988 GTATAC GTA^TAC
BstUI 62 CGCG CG^CG
BstZ17I 4988 GTATAC GTA^TAC
CfoI 61 GCGC GCG^C
Cfr10I 99 ACCGGC [GA]^CCGG[CT]
DrdI 1938 GACAATACTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 54 CGGCCG C^GGCCG
EclXI 54 CGGCCG C^GGCCG
Eco52I 54 CGGCCG C^GGCCG
EcoRV 2972 GATATC GAT^ATC
EcoT22I 4066 ATGCAT ATGCA^T
HhaI 61 GCGC GCG^C
Hin1I 1684 GACGTC G[GA]^CG[CT]C
HinP1I 61 GCGC G^CGC
HindIII 5476 AAGCTT A^AGCTT
HpaI 4587 GTTAAC GTT^AAC
MamI 543 GATGTTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MspA1I 1296 CAGCTG C[AC]G^C[GT]G
MvnI 62 CGCG CG^CG
NdeI 4420 CATATG CA^TATG
NheI 2781 GCTAGC G^CTAGC
NsiI 4066 ATGCAT ATGCA^T
PflFI 3323 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmeI 5368 GTTTAAAC GTTT^AAAC
PshAI 4688 GACATCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 1296 CAGCTG CAG^CTG
SacI 603 GAGCTC GAGCT^C
SapI 1667 GAAGAGC GCTCTTC(1/4)
SgrAI 98 CACCGGCG C[GA]^CCGG[CT]G
SmaI 44 CCCGGG CCC^GGG
Tth111I 3323 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 44 CCCGGG C^CCGGG
XmaI 44 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AccI 4988 GTATAC GT^[AC][GT]AC
5033 GTCTAC
Alw44I 1323 GTGCAC G^TGCAC
1803 GTGCAC
AlwNI 588 CAGTATCTG CAG[ACGT][ACGT][ACGT]^CTG
628 CAGCCACTG
ApaLI 1323 GTGCAC G^TGCAC
1803 GTGCAC
AseI 938 ATTAAT AT^TAAT
2686 ATTAAT
AsnI 938 ATTAAT AT^TAAT
2686 ATTAAT
AvaI 44 CCCGGG C^[CT]CG[GA]G
3425 CCCGAG
BalI 3506 TGGCCA TGG^CCA
4512 TGGCCA
BanI 96 GGCACC G^G[CT][GA]CC
205 GGTGCC
BbvCI 882 CCTCAGC CCTCAGC(-5/-2)
1596 GCTGAGG
BsaI 2818 GAGACC GGTCTC(1/5)
3516 GGTCTC
BsoBI 44 CCCGGG C^[CT]CG[GA]G
3425 CCCGAG
BspHI 293 TCATGA T^CATGA
4533 TCATGA
BsrBI 36 GAGCGG CCGCTC(-3/-3)
87 GAGCGG
MluNI 3506 TGGCCA TGG^CCA
4512 TGGCCA
MscI 3506 TGGCCA TGG^CCA
4512 TGGCCA
PflMI 3660 CCAAGGATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3693 CCATCTGTTGG
PpuMI 877 GGGACCC [GA]G^G[AT]CC[CT]
896 AGGACCT
PshBI 938 ATTAAT AT^TAAT
2686 ATTAAT
PstI 585 CTGCAG CTGCA^G
705 CTGCAG
RcaI 293 TCATGA T^CATGA
4533 TCATGA
SphI 1643 GCATGC GCATG^C
4068 GCATGC
StuI 256 AGGCCT AGG^CCT
687 AGGCCT
Van91I 3660 CCAAGGATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3693 CCATCTGTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 111 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI AocI ApoI Asp700I AspHI
AvaII BamHI BanII BbsI BbvI
BciVI BclI BcnI BfaI BglII
BmyI BpmI BpuAI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I Bsp1407I BspLU11I BsrDI
BsrGI BsrI BssKI BstAPI BstF5I
BstNI BstXI BstYI Bsu36I Cac8I
Cfr13I DdeI DpnII DraI DraII
DraIII DsaI EaeI EarI Eco81I
EcoO109I EcoRII EcoT14I FauI FbaI
Fnu4HI FokI HaeIII HapII HincII
HindII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspI MvaI MwoI NciI NcoI
NdeII NlaIII NlaIV NspI PleI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SspBI SspI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XbaI XhoII
XmnI