################################## # NO CUT ENZYMES ( 74 enzymes) # ################################## Acc65I AccIII AclI AgeI AhdI Aor51HI AscI Asp718I AspEI AviII AvrII BbeI BbrPI BlnI BlpI Bpu1102I BsaAI BsaWI BseAI BsiWI BspDI BspEI BssHII BssSI BstBI BstEII BstPI CelII ClaI CpoI Eam1105I Eco47III EcoNI EcoO65I EcoRI FseI FspI HaeII HgaI KasI KpnI KspI MfeI MluI MroI MunI NaeI NarI NgoMIV NotI NruI NspV PacI PaeR7I PinAI PmaCI PmlI Psp1406I PvuI RsrII SacII SalI SbfI ScaI SexAI SfiI SfoI SfuI SnaBI SpeI Sse8387I SwaI XcmI XhoI ################################### # ONE CUT ENZYMES ( 49 enzymes) # ################################### AatII 1684 GACGTC GACGT^C AccII 62 CGCG CG^CG AcyI 1684 GACGTC G[GA]^CG[CT]C AflII 2745 CTTAAG C^TTAAG ApaI 69 GGGCCC GGGCC^C AspI 3323 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC BcgI 4875 CGACACATTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BfrI 2745 CTTAAG C^TTAAG BglI 41 GCCCCCGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsaBI 543 GATGTTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 1684 GACGTC G[GA]^CG[CT]C BsiEI 54 CGGCCG CG[GA][CT]^CG BsmBI 3321 GAGACG CGTCTC(1/5) BsmI 1489 GCATTC GAATGC(1/-1) BspMI 3545 ACCTGC ACCTGC(4/8) BsrFI 99 ACCGGC [GA]^CCGG[CT] Bst1107I 4988 GTATAC GTA^TAC BstUI 62 CGCG CG^CG BstZ17I 4988 GTATAC GTA^TAC CfoI 61 GCGC GCG^C Cfr10I 99 ACCGGC [GA]^CCGG[CT] DrdI 1938 GACAATACTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EagI 54 CGGCCG C^GGCCG EclXI 54 CGGCCG C^GGCCG Eco52I 54 CGGCCG C^GGCCG EcoRV 2972 GATATC GAT^ATC EcoT22I 4066 ATGCAT ATGCA^T HhaI 61 GCGC GCG^C Hin1I 1684 GACGTC G[GA]^CG[CT]C HinP1I 61 GCGC G^CGC HindIII 5476 AAGCTT A^AGCTT HpaI 4587 GTTAAC GTT^AAC MamI 543 GATGTTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MspA1I 1296 CAGCTG C[AC]G^C[GT]G MvnI 62 CGCG CG^CG NdeI 4420 CATATG CA^TATG NheI 2781 GCTAGC G^CTAGC NsiI 4066 ATGCAT ATGCA^T PflFI 3323 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC PmeI 5368 GTTTAAAC GTTT^AAAC PshAI 4688 GACATCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PvuII 1296 CAGCTG CAG^CTG SacI 603 GAGCTC GAGCT^C SapI 1667 GAAGAGC GCTCTTC(1/4) SgrAI 98 CACCGGCG C[GA]^CCGG[CT]G SmaI 44 CCCGGG CCC^GGG Tth111I 3323 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC XmaCI 44 CCCGGG C^CCGGG XmaI 44 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### AccI 4988 GTATAC GT^[AC][GT]AC 5033 GTCTAC Alw44I 1323 GTGCAC G^TGCAC 1803 GTGCAC AlwNI 588 CAGTATCTG CAG[ACGT][ACGT][ACGT]^CTG 628 CAGCCACTG ApaLI 1323 GTGCAC G^TGCAC 1803 GTGCAC AseI 938 ATTAAT AT^TAAT 2686 ATTAAT AsnI 938 ATTAAT AT^TAAT 2686 ATTAAT AvaI 44 CCCGGG C^[CT]CG[GA]G 3425 CCCGAG BalI 3506 TGGCCA TGG^CCA 4512 TGGCCA BanI 96 GGCACC G^G[CT][GA]CC 205 GGTGCC BbvCI 882 CCTCAGC CCTCAGC(-5/-2) 1596 GCTGAGG BsaI 2818 GAGACC GGTCTC(1/5) 3516 GGTCTC BsoBI 44 CCCGGG C^[CT]CG[GA]G 3425 CCCGAG BspHI 293 TCATGA T^CATGA 4533 TCATGA BsrBI 36 GAGCGG CCGCTC(-3/-3) 87 GAGCGG MluNI 3506 TGGCCA TGG^CCA 4512 TGGCCA MscI 3506 TGGCCA TGG^CCA 4512 TGGCCA PflMI 3660 CCAAGGATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3693 CCATCTGTTGG PpuMI 877 GGGACCC [GA]G^G[AT]CC[CT] 896 AGGACCT PshBI 938 ATTAAT AT^TAAT 2686 ATTAAT PstI 585 CTGCAG CTGCA^G 705 CTGCAG RcaI 293 TCATGA T^CATGA 4533 TCATGA SphI 1643 GCATGC GCATG^C 4068 GCATGC StuI 256 AGGCCT AGG^CCT 687 AGGCCT Van91I 3660 CCAAGGATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3693 CCATCTGTTGG ######################################### # 3 OR MORE CUT ENZYMES ( 111 enzymes) # ######################################### AciI AcsI AfaI AflIII AluI AlwI AocI ApoI Asp700I AspHI AvaII BamHI BanII BbsI BbvI BciVI BclI BcnI BfaI BglII BmyI BpmI BpuAI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I Bsp1407I BspLU11I BsrDI BsrGI BsrI BssKI BstAPI BstF5I BstNI BstXI BstYI Bsu36I Cac8I Cfr13I DdeI DpnII DraI DraII DraIII DsaI EaeI EarI Eco81I EcoO109I EcoRII EcoT14I FauI FbaI Fnu4HI FokI HaeIII HapII HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspI MvaI MwoI NciI NcoI NdeII NlaIII NlaIV NspI PleI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SspBI SspI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XbaI XhoII XmnI