##################################
# NO CUT ENZYMES ( 99 enzymes) #
##################################
AatII Acc65I AclI AflII AgeI
AhdI AocI AseI AsnI Asp718I
AspEI AspI AviII AvrII BbeI
BbvCI BclI BfrI BglII BlnI
BsaBI BsiEI BsiWI BspDI BspHI
BspLU11I BsrFI Bst1107I BstXI BstZ17I
Bsu36I Cfr10I ClaI DraI DrdI
EagI Eam1105I EarI EclXI Eco52I
Eco81I EcoNI EcoT14I EcoT22I FbaI
FseI FspI HindIII HpaI KasI
KpnI Ksp632I MamI MfeI MluI
MunI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
PacI PaeR7I PflFI PinAI PmeI
PshAI PshBI Psp1406I PstI PvuI
PvuII RcaI SacI SalI SapI
SbfI ScaI SexAI SfiI SfoI
SgrAI SmlI SnaBI SpeI SphI
Sse8387I SspI StuI StyI SwaI
Tth111I XbaI XcmI XhoI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
AccI 1674 GTCTAC GT^[AC][GT]AC
AcyI 570 GGCGTC G[GA]^CG[CT]C
AflIII 1601 ACGTGT A^C[GA][CT]GT
Alw44I 138 GTGCAC G^TGCAC
AlwNI 1066 CAGCCGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 558 GGGCCC GGGCC^C
ApaLI 138 GTGCAC G^TGCAC
AscI 722 GGCGCGCC GG^CGCGCC
BalI 1319 TGGCCA TGG^CCA
BamHI 1409 GGATCC G^GATCC
BbrPI 1600 CACGTG CAC^GTG
BbsI 999 GAAGAC GAAGAC(2/6)
BfaI 896 CTAG C^TAG
BlpI 1089 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1089 GCTCAGC GC^T[ACGT]AGC
BpuAI 999 GAAGAC GAAGAC(2/6)
BsaAI 1600 CACGTG [CT]AC^GT[GA]
BsaHI 570 GGCGTC G[GA]^CG[CT]C
BsaI 178 GGTCTC GGTCTC(1/5)
BseRI 246 CTCCTC GAGGAG(10/8)
BsmBI 1277 CGTCTC CGTCTC(1/5)
BsmI 956 GCATTC GAATGC(1/-1)
Bsp1407I 1741 TGTACA T^GTACA
BsrDI 1776 GCAATG GCAATG(2/0)
BsrGI 1741 TGTACA T^GTACA
BssSI 1262 CACGAG CACGAG(-5/-1)
BstBI 1509 TTCGAA TT^CGAA
BstEII 202 GGTCACC G^GT[ACGT]ACC
BstPI 202 GGTCACC G^GT[ACGT]ACC
CelII 1089 GCTCAGC GC^T[ACGT]AGC
CpoI 624 CGGACCG CG^G[AT]CCG
DraIII 668 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoO65I 202 GGTCACC G^GT[ACGT]ACC
EcoRV 963 GATATC GAT^ATC
Hin1I 570 GGCGTC G[GA]^CG[CT]C
HincII 1626 GTCAAC GT[CT]^[GA]AC
HindII 1626 GTCAAC GT[CT]^[GA]AC
MaeI 896 CTAG C^TAG
MluNI 1319 TGGCCA TGG^CCA
MscI 1319 TGGCCA TGG^CCA
NspV 1509 TTCGAA TT^CGAA
PmaCI 1600 CACGTG CAC^GTG
PmlI 1600 CACGTG CAC^GTG
PpuMI 684 GGGTCCC [GA]G^G[AT]CC[CT]
RsrII 624 CGGACCG CG^G[AT]CCG
SfaNI 1524 GATGC GCATC(5/9)
SfcI 1676 CTACAG C^T[GA][CT]AG
SfuI 1509 TTCGAA TT^CGAA
SspBI 1741 TGTACA T^GTACA
###################################
# TWO CUT ENZYMES ( 35 enzymes) #
###################################
AcsI 842 GAATTC [GA]^AATT[CT]
1686 GAATTC
Aor51HI 116 AGCGCT AGC^GCT
1266 AGCGCT
ApoI 842 GAATTC [GA]^AATT[CT]
1686 GAATTC
Asp700I 286 GAAGTGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1006 GAAAGGTTTC
BanI 1284 GGCACC G^G[CT][GA]CC
1554 GGCACC
BciVI 1132 GTATCC GTATCC(6/5)
1793 GTATCC
BglI 487 GCCTCCGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1367 GCCCAAGCGGC
BsgI 825 GTGCAG GTGCAG(16/14)
1669 GTGCAG
BspMI 742 ACCTGC ACCTGC(4/8)
984 ACCTGC
BsrBI 147 CCGCTC CCGCTC(-3/-3)
621 GAGCGG
BstAPI 979 GCAATACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1065 GCAGCCGCTGC
BstF5I 1158 CATCC GGATG(2/0)
1316 GGATG
BstYI 1409 GGATCC [GA]^GATC[CT]
1731 GGATCT
DraII 558 GGGCCCC [GA]G^G[ACGT]CC[CT]
684 GGGTCCC
Eco47III 116 AGCGCT AGC^GCT
1266 AGCGCT
EcoO109I 558 GGGCCCC [GA]G^G[ACGT]CC[CT]
684 GGGTCCC
EcoRI 842 GAATTC G^AATTC
1686 GAATTC
FokI 1158 CATCC GGATG(9/13)
1316 GGATG
KspI 413 CCGCGG CCGC^GG
1138 CCGCGG
MaeII 866 ACGT A^CGT
1601 ACGT
MflI 1409 GGATCC [GA]^GATC[CT]
1731 GGATCT
MseI 1212 TTAA T^TAA
1471 TTAA
MslI 7 CACACACATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
918 CATGCAAGTG
NspI 12 ACATGC [GA]CATG^[CT]
1379 GCATGT
PflMI 667 CCACCCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
799 CCAGCTTATGG
SacII 413 CCGCGG CCGC^GG
1138 CCGCGG
SmaI 442 CCCGGG CCC^GGG
635 CCCGGG
TfiI 376 GAATC G^A[AT]TC
1655 GAATC
Tru9I 1212 TTAA T^TAA
1471 TTAA
Tsp45I 203 GTCAC ^GT[GC]AC
868 GTGAC
Van91I 667 CCACCCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
799 CCAGCTTATGG
XhoII 1409 GGATCC [GA]^GATC[CT]
1731 GGATCT
XmaCI 442 CCCGGG C^CCGGG
635 CCCGGG
XmaI 442 CCCGGG C^CCGGG
635 CCCGGG
XmnI 286 GAAGTGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1006 GAAAGGTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 75 enzymes) #
#########################################
AccII AccIII AciI AfaI AluI
AlwI AspHI AvaI AvaII BanII
BbvI BcgI BcnI BmyI BpmI
BsaJI BsaWI BseAI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspEI BsrI BssHII BssKI BstNI
BstUI Cac8I CfoI Cfr13I DdeI
DpnII DsaI EaeI EcoRII FauI
Fnu4HI HaeII HaeIII HapII HgaI
HhaI HinP1I HinfI HpaII HphI
ItaI MaeIII MboI MboII MnlI
MroI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
PleI RsaI Sau3AI Sau96I ScrFI
TaqI TseI Tsp509I TspRI TthHB8I