################################## # NO CUT ENZYMES ( 99 enzymes) # ################################## AatII Acc65I AclI AflII AgeI AhdI AocI AseI AsnI Asp718I AspEI AspI AviII AvrII BbeI BbvCI BclI BfrI BglII BlnI BsaBI BsiEI BsiWI BspDI BspHI BspLU11I BsrFI Bst1107I BstXI BstZ17I Bsu36I Cfr10I ClaI DraI DrdI EagI Eam1105I EarI EclXI Eco52I Eco81I EcoNI EcoT14I EcoT22I FbaI FseI FspI HindIII HpaI KasI KpnI Ksp632I MamI MfeI MluI MunI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI PaeR7I PflFI PinAI PmeI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI SacI SalI SapI SbfI ScaI SexAI SfiI SfoI SgrAI SmlI SnaBI SpeI SphI Sse8387I SspI StuI StyI SwaI Tth111I XbaI XcmI XhoI ################################### # ONE CUT ENZYMES ( 49 enzymes) # ################################### AccI 1674 GTCTAC GT^[AC][GT]AC AcyI 570 GGCGTC G[GA]^CG[CT]C AflIII 1601 ACGTGT A^C[GA][CT]GT Alw44I 138 GTGCAC G^TGCAC AlwNI 1066 CAGCCGCTG CAG[ACGT][ACGT][ACGT]^CTG ApaI 558 GGGCCC GGGCC^C ApaLI 138 GTGCAC G^TGCAC AscI 722 GGCGCGCC GG^CGCGCC BalI 1319 TGGCCA TGG^CCA BamHI 1409 GGATCC G^GATCC BbrPI 1600 CACGTG CAC^GTG BbsI 999 GAAGAC GAAGAC(2/6) BfaI 896 CTAG C^TAG BlpI 1089 GCTCAGC GC^T[ACGT]AGC Bpu1102I 1089 GCTCAGC GC^T[ACGT]AGC BpuAI 999 GAAGAC GAAGAC(2/6) BsaAI 1600 CACGTG [CT]AC^GT[GA] BsaHI 570 GGCGTC G[GA]^CG[CT]C BsaI 178 GGTCTC GGTCTC(1/5) BseRI 246 CTCCTC GAGGAG(10/8) BsmBI 1277 CGTCTC CGTCTC(1/5) BsmI 956 GCATTC GAATGC(1/-1) Bsp1407I 1741 TGTACA T^GTACA BsrDI 1776 GCAATG GCAATG(2/0) BsrGI 1741 TGTACA T^GTACA BssSI 1262 CACGAG CACGAG(-5/-1) BstBI 1509 TTCGAA TT^CGAA BstEII 202 GGTCACC G^GT[ACGT]ACC BstPI 202 GGTCACC G^GT[ACGT]ACC CelII 1089 GCTCAGC GC^T[ACGT]AGC CpoI 624 CGGACCG CG^G[AT]CCG DraIII 668 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG EcoO65I 202 GGTCACC G^GT[ACGT]ACC EcoRV 963 GATATC GAT^ATC Hin1I 570 GGCGTC G[GA]^CG[CT]C HincII 1626 GTCAAC GT[CT]^[GA]AC HindII 1626 GTCAAC GT[CT]^[GA]AC MaeI 896 CTAG C^TAG MluNI 1319 TGGCCA TGG^CCA MscI 1319 TGGCCA TGG^CCA NspV 1509 TTCGAA TT^CGAA PmaCI 1600 CACGTG CAC^GTG PmlI 1600 CACGTG CAC^GTG PpuMI 684 GGGTCCC [GA]G^G[AT]CC[CT] RsrII 624 CGGACCG CG^G[AT]CCG SfaNI 1524 GATGC GCATC(5/9) SfcI 1676 CTACAG C^T[GA][CT]AG SfuI 1509 TTCGAA TT^CGAA SspBI 1741 TGTACA T^GTACA ################################### # TWO CUT ENZYMES ( 35 enzymes) # ################################### AcsI 842 GAATTC [GA]^AATT[CT] 1686 GAATTC Aor51HI 116 AGCGCT AGC^GCT 1266 AGCGCT ApoI 842 GAATTC [GA]^AATT[CT] 1686 GAATTC Asp700I 286 GAAGTGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1006 GAAAGGTTTC BanI 1284 GGCACC G^G[CT][GA]CC 1554 GGCACC BciVI 1132 GTATCC GTATCC(6/5) 1793 GTATCC BglI 487 GCCTCCGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 1367 GCCCAAGCGGC BsgI 825 GTGCAG GTGCAG(16/14) 1669 GTGCAG BspMI 742 ACCTGC ACCTGC(4/8) 984 ACCTGC BsrBI 147 CCGCTC CCGCTC(-3/-3) 621 GAGCGG BstAPI 979 GCAATACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 1065 GCAGCCGCTGC BstF5I 1158 CATCC GGATG(2/0) 1316 GGATG BstYI 1409 GGATCC [GA]^GATC[CT] 1731 GGATCT DraII 558 GGGCCCC [GA]G^G[ACGT]CC[CT] 684 GGGTCCC Eco47III 116 AGCGCT AGC^GCT 1266 AGCGCT EcoO109I 558 GGGCCCC [GA]G^G[ACGT]CC[CT] 684 GGGTCCC EcoRI 842 GAATTC G^AATTC 1686 GAATTC FokI 1158 CATCC GGATG(9/13) 1316 GGATG KspI 413 CCGCGG CCGC^GG 1138 CCGCGG MaeII 866 ACGT A^CGT 1601 ACGT MflI 1409 GGATCC [GA]^GATC[CT] 1731 GGATCT MseI 1212 TTAA T^TAA 1471 TTAA MslI 7 CACACACATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 918 CATGCAAGTG NspI 12 ACATGC [GA]CATG^[CT] 1379 GCATGT PflMI 667 CCACCCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 799 CCAGCTTATGG SacII 413 CCGCGG CCGC^GG 1138 CCGCGG SmaI 442 CCCGGG CCC^GGG 635 CCCGGG TfiI 376 GAATC G^A[AT]TC 1655 GAATC Tru9I 1212 TTAA T^TAA 1471 TTAA Tsp45I 203 GTCAC ^GT[GC]AC 868 GTGAC Van91I 667 CCACCCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 799 CCAGCTTATGG XhoII 1409 GGATCC [GA]^GATC[CT] 1731 GGATCT XmaCI 442 CCCGGG C^CCGGG 635 CCCGGG XmaI 442 CCCGGG C^CCGGG 635 CCCGGG XmnI 286 GAAGTGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1006 GAAAGGTTTC ######################################### # 3 OR MORE CUT ENZYMES ( 75 enzymes) # ######################################### AccII AccIII AciI AfaI AluI AlwI AspHI AvaI AvaII BanII BbvI BcgI BcnI BmyI BpmI BsaJI BsaWI BseAI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspEI BsrI BssHII BssKI BstNI BstUI Cac8I CfoI Cfr13I DdeI DpnII DsaI EaeI EcoRII FauI Fnu4HI HaeII HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI MaeIII MboI MboII MnlI MroI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV PleI RsaI Sau3AI Sau96I ScrFI TaqI TseI Tsp509I TspRI TthHB8I