##################################
# NO CUT ENZYMES ( 78 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AgeI AhdI Alw44I Aor51HI ApaLI
AscI Asp718I AspEI AvaI AviII
BbrPI BcgI BglI BsaBI BsaWI
BseAI BsiEI BsiWI BsmBI BsoBI
BspEI BsrBI BsrFI BssHII Bst1107I
BstBI BstUI BstZ17I Cfr10I CpoI
DrdI EagI Eam1105I EclXI Eco47III
Eco52I EcoRI FseI FspI HindIII
KpnI KspI MamI MluI MroI
MvnI NaeI NdeI NgoMIV NheI
NotI NruI NspV PaeR7I PinAI
PmaCI PmeI PmlI PvuI RsrII
SacII SalI SbfI SfiI SfuI
SgrAI SmaI SphI Sse8387I SwaI
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AclI 3584 AACGTT AA^CGTT
AcyI 1910 GGCGCC G[GA]^CG[CT]C
AflII 3937 CTTAAG C^TTAAG
ApaI 4647 GGGCCC GGGCC^C
AseI 3068 ATTAAT AT^TAAT
AsnI 3068 ATTAAT AT^TAAT
AvrII 4143 CCTAGG C^CTAGG
BamHI 3967 GGATCC G^GATCC
BbeI 1910 GGCGCC GGCGC^C
BclI 2740 TGATCA T^GATCA
BfrI 3937 CTTAAG C^TTAAG
BlnI 4143 CCTAGG C^CTAGG
BsaAI 2664 TACGTA [CT]AC^GT[GA]
BsaHI 1910 GGCGCC G[GA]^CG[CT]C
BspDI 2006 ATCGAT AT^CGAT
BspHI 351 TCATGA T^CATGA
BssSI 1455 CTCGTG CACGAG(-5/-1)
BstAPI 1286 GCAACCTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
ClaI 2006 ATCGAT AT^CGAT
EcoRV 1571 GATATC GAT^ATC
EcoT22I 4597 ATGCAT ATGCA^T
FauI 1466 CCCGC CCCGC(4/6)
FbaI 2740 TGATCA T^GATCA
HaeII 1910 GGCGCC [GA]GCGC^[CT]
HgaI 5161 GCGTC GACGC(5/10)
Hin1I 1910 GGCGCC G[GA]^CG[CT]C
HpaI 517 GTTAAC GTT^AAC
KasI 1910 GGCGCC G^GCGCC
MfeI 4121 CAATTG C^AATTG
MunI 4121 CAATTG C^AATTG
NarI 1910 GGCGCC GG^CGCC
NsiI 4597 ATGCAT ATGCA^T
PshAI 5485 GACAGTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 3068 ATTAAT AT^TAAT
Psp1406I 3584 AACGTT AA^CGTT
PstI 4725 CTGCAG CTGCA^G
RcaI 351 TCATGA T^CATGA
SacI 4800 GAGCTC GAGCT^C
SapI 3154 GAAGAGC GCTCTTC(1/4)
SexAI 2799 ACCAGGT A^CC[AT]GGT
SfoI 1910 GGCGCC GGC^GCC
SnaBI 2664 TACGTA TAC^GTA
StuI 5090 AGGCCT AGG^CCT
###################################
# TWO CUT ENZYMES ( 44 enzymes) #
###################################
AflIII 231 ACATGT A^C[GA][CT]GT
4733 ACATGT
AocI 1030 CCTCAGG CC^T[ACGT]AGG
2515 CCTGAGG
Asp700I 2341 GAAACTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4922 GAACTCCTTC
AspHI 4800 GAGCTC G[AT]GC[AT]^C
5139 GTGCTC
AspI 2954 GACTAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
3821 GACTGGGTC
BalI 1004 TGGCCA TGG^CCA
2143 TGGCCA
BbsI 5288 GAAGAC GAAGAC(2/6)
3238 GTCTTC
BbvCI 1322 CCTCAGC CCTCAGC(-5/-2)
3757 CCTCAGC
BcnI 1093 CCCGG CC^[GC]GG
4805 CCGGG
BglII 266 AGATCT A^GATCT
3746 AGATCT
BlpI 2134 GCTAAGC GC^T[ACGT]AGC
3680 GCTAAGC
Bpu1102I 2134 GCTAAGC GC^T[ACGT]AGC
3680 GCTAAGC
BpuAI 5288 GAAGAC GAAGAC(2/6)
3238 GTCTTC
BsiHKAI 4800 GAGCTC G[AT]GC[AT]^C
5139 GTGCTC
Bsp1407I 1728 TGTACA T^GTACA
2243 TGTACA
BspLU11I 231 ACATGT A^CATGT
4733 ACATGT
BspMI 1043 ACCTGC ACCTGC(4/8)
1354 ACCTGC
BsrGI 1728 TGTACA T^GTACA
2243 TGTACA
BstEII 4253 GGTAACC G^GT[ACGT]ACC
4453 GGTTACC
BstPI 4253 GGTAACC G^GT[ACGT]ACC
4453 GGTTACC
Bsu36I 1030 CCTCAGG CC^T[ACGT]AGG
2515 CCTGAGG
CelII 2134 GCTAAGC GC^T[ACGT]AGC
3680 GCTAAGC
CfoI 1512 GCGC GCG^C
1911 GCGC
DraIII 2549 CACAAGGTG CAC[ACGT][ACGT][ACGT]^GTG
2798 CACCAGGTG
EaeI 1004 TGGCCA [CT]^GGCC[GA]
2143 TGGCCA
Eco81I 1030 CCTCAGG CC^T[ACGT]AGG
2515 CCTGAGG
EcoNI 5355 CCTGAGCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
5447 CCTCGACAAGG
EcoO65I 4253 GGTAACC G^GT[ACGT]ACC
4453 GGTTACC
HapII 1094 CCGG C^CGG
4805 CCGG
HhaI 1512 GCGC GCG^C
1911 GCGC
HinP1I 1512 GCGC G^CGC
1911 GCGC
HpaII 1094 CCGG C^CGG
4805 CCGG
MaeII 2665 ACGT A^CGT
3585 ACGT
MluNI 1004 TGGCCA TGG^CCA
2143 TGGCCA
MscI 1004 TGGCCA TGG^CCA
2143 TGGCCA
MspI 1094 CCGG C^CGG
4805 CCGG
NciI 1093 CCCGG CC^[GC]GG
4805 CCGGG
PacI 385 TTAATTAA TTAAT^TAA
3363 TTAATTAA
PflFI 2954 GACTAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
3821 GACTGGGTC
PpuMI 1552 GGGACCC [GA]G^G[AT]CC[CT]
5039 AGGTCCT
SspBI 1728 TGTACA T^GTACA
2243 TGTACA
Tth111I 2954 GACTAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
3821 GACTGGGTC
XcmI 416 CCATGTTGTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
4879 CCAAGGATATGTTGG
XmnI 2341 GAAACTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4922 GAACTCCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 93 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI AvaII BanI BanII
BbvI BciVI BfaI BmyI BpmI
BsaI BsaJI BseRI BsgI BsiYI
BslI BsmAI BsmFI BsmI Bsp1286I
BsrDI BsrI BssKI BstF5I BstNI
BstXI BstYI Cac8I Cfr13I DdeI
DpnII DraI DraII DsaI EarI
EcoO109I EcoRII EcoT14I Fnu4HI FokI
HaeIII HincII HindII HinfI HphI
ItaI Ksp632I MaeI MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MvaI MwoI NcoI NdeII
NlaIII NlaIV NspI PflMI PleI
PvuII RsaI Sau3AI Sau96I ScaI
ScrFI SfaNI SfcI SmlI SpeI
SspI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XbaI XhoII