##################################
# NO CUT ENZYMES ( 53 enzymes) #
##################################
AatII Acc65I AccI AclI AflII
AhdI Asp700I Asp718I AspEI BfrI
BlpI Bpu1102I BsiWI Bsp1407I BspDI
BspLU11I BsrGI Bst1107I BstBI BstZ17I
CelII ClaI DrdI Eam1105I EcoRV
FseI HincII HindII HpaI KpnI
MfeI MluI MunI NheI NotI
NruI NspV PacI PmeI PshAI
Psp1406I PvuI SalI ScaI SfiI
SfuI SgrAI SnaBI SpeI SspBI
SspI SwaI XmnI
###################################
# ONE CUT ENZYMES ( 35 enzymes) #
###################################
AgeI 4154 ACCGGT A^CCGGT
Alw44I 5116 GTGCAC G^TGCAC
ApaLI 5116 GTGCAC G^TGCAC
AscI 3207 GGCGCGCC GG^CGCGCC
AseI 448 ATTAAT AT^TAAT
AsnI 448 ATTAAT AT^TAAT
AvrII 696 CCTAGG C^CTAGG
BclI 5301 TGATCA T^GATCA
BglII 2346 AGATCT A^GATCT
BlnI 696 CCTAGG C^CTAGG
BsaBI 3580 GATCAGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsrDI 4899 CATTGC GCAATG(2/0)
BstAPI 3699 GCAGTCCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CpoI 2719 CGGTCCG CG^G[AT]CCG
EagI 2806 CGGCCG C^GGCCG
EclXI 2806 CGGCCG C^GGCCG
Eco52I 2806 CGGCCG C^GGCCG
EcoRI 2153 GAATTC G^AATTC
FbaI 5301 TGATCA T^GATCA
KspI 89 CCGCGG CCGC^GG
MamI 3580 GATCAGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NaeI 2712 GCCGGC GCC^GGC
NcoI 62 CCATGG C^CATGG
NdeI 3257 CATATG CA^TATG
NgoMIV 2712 GCCGGC G^CCGGC
PaeR7I 2642 CTCGAG C^TCGAG
PinAI 4154 ACCGGT A^CCGGT
PshBI 448 ATTAAT AT^TAAT
RsrII 2719 CGGTCCG CG^G[AT]CCG
SacII 89 CCGCGG CCGC^GG
SapI 2284 GCTCTTC GCTCTTC(1/4)
SbfI 1216 CCTGCAGG CCTGCA^GG
Sse8387I 1216 CCTGCAGG CCTGCA^GG
XbaI 2069 TCTAGA T^CTAGA
XhoI 2642 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AccIII 333 TCCGGA T^CCGGA
4096 TCCGGA
AfaI 2969 GTAC GT^AC
5179 GTAC
Aor51HI 2124 AGCGCT AGC^GCT
3335 AGCGCT
AspI 1052 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
5686 GACTCCGTC
AviII 3277 TGCGCA TGC^GCA
3851 TGCGCA
BamHI 1092 GGATCC G^GATCC
1143 GGATCC
BbsI 129 GAAGAC GAAGAC(2/6)
2910 GTCTTC
BbvCI 769 CCTCAGC CCTCAGC(-5/-2)
5595 GCTGAGG
BciVI 330 GTATCC GTATCC(6/5)
1576 GTATCC
BpuAI 129 GAAGAC GAAGAC(2/6)
2910 GTCTTC
BseAI 333 TCCGGA T^CCGGA
4096 TCCGGA
BsiEI 2458 CGGTCG CG[GA][CT]^CG
2806 CGGCCG
BsmBI 2996 CGTCTC CGTCTC(1/5)
5524 CGTCTC
BspEI 333 TCCGGA T^CCGGA
4096 TCCGGA
BspHI 47 TCATGA T^CATGA
5489 TCATGA
BssHII 3208 GCGCGC G^CGCGC
3385 GCGCGC
DraIII 4431 CACTGCGTG CAC[ACGT][ACGT][ACGT]^GTG
5294 CACCTAGTG
Eco47III 2124 AGCGCT AGC^GCT
3335 AGCGCT
EcoNI 2217 CCTCCTTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3705 CCTGCGCGAGG
FspI 3277 TGCGCA TGC^GCA
3851 TGCGCA
HindIII 482 AAGCTT A^AGCTT
1789 AAGCTT
MroI 333 TCCGGA T^CCGGA
4096 TCCGGA
PflFI 1052 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
5686 GACTCCGTC
RcaI 47 TCATGA T^CATGA
5489 TCATGA
RsaI 2969 GTAC GT^AC
5179 GTAC
SacI 4199 GAGCTC GAGCT^C
5289 GAGCTC
SexAI 4510 ACCAGGT A^CC[AT]GGT
4572 ACCTGGT
SphI 1257 GCATGC GCATG^C
3301 GCATGC
StuI 4028 AGGCCT AGG^CCT
4646 AGGCCT
Tth111I 1052 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
5686 GACTCCGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 140 enzymes) #
#########################################
AccII AciI AcsI AcyI AflIII
AluI AlwI AlwNI AocI ApaI
ApoI AspHI AvaI AvaII BalI
BanI BanII BbeI BbrPI BbvI
BcgI BcnI BfaI BglI BmyI
BpmI BsaAI BsaHI BsaI BsaJI
BsaWI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsmI BsoBI
Bsp1286I BspMI BsrBI BsrFI BsrI
BssKI BssSI BstEII BstF5I BstNI
BstPI BstUI BstXI BstYI Bsu36I
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraI DraII DsaI EaeI
EarI Eco81I EcoO109I EcoO65I EcoRII
EcoT14I EcoT22I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI KasI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NarI
NciI NdeII NlaIII NlaIV NsiI
NspI PflMI PleI PmaCI PmlI
PpuMI PstI PvuII Sau3AI Sau96I
ScrFI SfaNI SfcI SfoI SmaI
SmlI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XcmI XhoII XmaCI XmaI