##################################
# NO CUT ENZYMES ( 73 enzymes) #
##################################
AatII AclI AcyI AflII AgeI
Alw44I Aor51HI ApaLI AscI AspI
AviII BbeI BcgI BfrI BglI
BsaHI BsaI BsiWI BsmBI Bsp1407I
BspHI BsrGI Bst1107I BstBI BstZ17I
DsaI Eco47III EcoNI FseI FspI
HaeII Hin1I KasI KspI MluI
NarI NcoI NotI NruI NspV
PacI PaeR7I PflFI PflMI PinAI
PmeI PpuMI PshAI Psp1406I PvuI
RcaI SacI SacII SalI SbfI
SexAI SfiI SfoI SfuI SgrAI
SmaI SpeI SphI Sse8387I SspBI
StuI SwaI Tth111I Van91I XbaI
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
AccI 1349 GTCTAC GT^[AC][GT]AC
AccIII 4784 TCCGGA T^CCGGA
AhdI 1972 GACTTCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 3481 CAGAAACTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 178 GGGCCC GGGCC^C
AseI 3468 ATTAAT AT^TAAT
AsnI 3468 ATTAAT AT^TAAT
AspEI 1972 GACTTCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 3903 GAGCAC G[AT]GC[AT]^C
AvrII 3192 CCTAGG C^CTAGG
BalI 3917 TGGCCA TGG^CCA
BbrPI 1588 CACGTG CAC^GTG
BbvCI 139 GCTGAGG CCTCAGC(-5/-2)
BlnI 3192 CCTAGG C^CTAGG
BlpI 5179 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 5179 GCTGAGC GC^T[ACGT]AGC
BsaBI 4623 GATGGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 4784 TCCGGA T^CCGGA
BsiEI 368 CGGCCG CG[GA][CT]^CG
BsiHKAI 3903 GAGCAC G[AT]GC[AT]^C
BspEI 4784 TCCGGA T^CCGGA
BsrBI 148 GAGCGG CCGCTC(-3/-3)
BsrDI 1212 GCAATG GCAATG(2/0)
BssSI 1895 CACGAG CACGAG(-5/-1)
BstAPI 501 GCATTTGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CelII 5179 GCTGAGC GC^T[ACGT]AGC
CpoI 22 CGGTCCG CG^G[AT]CCG
DraIII 3036 CACTTGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 4387 GACCTCATTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 368 CGGCCG C^GGCCG
Eam1105I 1972 GACTTCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 368 CGGCCG C^GGCCG
Eco52I 368 CGGCCG C^GGCCG
EcoRI 4746 GAATTC G^AATTC
MamI 4623 GATGGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 3917 TGGCCA TGG^CCA
MroI 4784 TCCGGA T^CCGGA
MscI 3917 TGGCCA TGG^CCA
NdeI 5027 CATATG CA^TATG
NheI 3574 GCTAGC G^CTAGC
PmaCI 1588 CACGTG CAC^GTG
PmlI 1588 CACGTG CAC^GTG
PshBI 3468 ATTAAT AT^TAAT
PvuII 5399 CAGCTG CAG^CTG
RsrII 22 CGGTCCG CG^G[AT]CCG
SapI 4180 GAAGAGC GCTCTTC(1/4)
SnaBI 3628 TACGTA TAC^GTA
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
Acc65I 3207 GGTACC G^GTACC
3745 GGTACC
AocI 172 CCTCAGG CC^T[ACGT]AGG
556 CCTCAGG
Asp718I 3207 GGTACC G^GTACC
3745 GGTACC
AvaI 61 CTCGGG C^[CT]CG[GA]G
3764 CCCGAG
AvaII 23 GGTCC G^G[AT]CC
3257 GGTCC
BciVI 489 GGATAC GTATCC(6/5)
3241 GGATAC
BglII 1676 AGATCT A^GATCT
3313 AGATCT
BsmI 703 GAATGC GAATGC(1/-1)
1526 GAATGC
BsoBI 61 CTCGGG C^[CT]CG[GA]G
3764 CCCGAG
BspDI 4291 ATCGAT AT^CGAT
4495 ATCGAT
BspMI 1475 GCAGGT ACCTGC(4/8)
2262 GCAGGT
BstEII 1707 GGTTACC G^GT[ACGT]ACC
5163 GGTAACC
BstPI 1707 GGTTACC G^GT[ACGT]ACC
5163 GGTAACC
Bsu36I 172 CCTCAGG CC^T[ACGT]AGG
556 CCTCAGG
ClaI 4291 ATCGAT AT^CGAT
4495 ATCGAT
DraII 177 GGGGCCC [GA]G^G[ACGT]CC[CT]
4238 AGGGCCT
Eco81I 172 CCTCAGG CC^T[ACGT]AGG
556 CCTCAGG
EcoO109I 177 GGGGCCC [GA]G^G[ACGT]CC[CT]
4238 AGGGCCT
EcoO65I 1707 GGTTACC G^GT[ACGT]ACC
5163 GGTAACC
EcoRV 5101 GATATC GAT^ATC
5362 GATATC
HgaI 99 GACGC GACGC(5/10)
295 GACGC
HindIII 2533 AAGCTT A^AGCTT
3998 AAGCTT
HpaI 3585 GTTAAC GTT^AAC
4153 GTTAAC
KpnI 3207 GGTACC GGTAC^C
3745 GGTACC
MslI 1466 CATCAAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
4309 CATAAAGGTG
MspA1I 183 CAGCGG C[AC]G^C[GT]G
5399 CAGCTG
NaeI 4 GCCGGC GCC^GGC
47 GCCGGC
NgoMIV 4 GCCGGC G^CCGGC
47 GCCGGC
PstI 3322 CTGCAG CTGCA^G
4985 CTGCAG
ScaI 3606 AGTACT AGT^ACT
5201 AGTACT
SmlI 1246 CTCAAG C^T[CT][GA]AG
4278 CTTGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 107 enzymes) #
#########################################
AccII AciI AcsI AfaI AflIII
AluI AlwI ApoI Asp700I BamHI
BanI BanII BbsI BbvI BclI
BcnI BfaI BmyI BpmI BpuAI
BsaAI BsaJI BsaWI BseRI BsgI
BsiYI BslI BsmAI BsmFI Bsp1286I
BspLU11I BsrFI BsrI BssHII BssKI
BstF5I BstNI BstUI BstXI BstYI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraI EaeI EarI EcoRII
EcoT14I EcoT22I FauI FbaI Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HincII HindII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MfeI MflI MnlI
MseI MspI MunI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
NsiI NspI PleI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SspI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XcmI
XhoII XmnI