##################################
# NO CUT ENZYMES ( 69 enzymes) #
##################################
AatII AccI AccIII AclI AgeI
AhdI AscI AspEI AviII AvrII
BamHI BbeI BciVI BlnI BsaBI
BseAI BsiWI Bsp1407I BspDI BspEI
BspHI BsrGI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstZ17I ClaI
CpoI Eam1105I EcoO65I EcoRV FseI
FspI KasI KspI MamI MfeI
MluI MroI MunI NarI NotI
NruI NspV PaeR7I PinAI PmeI
PshAI Psp1406I PvuI RcaI RsrII
SacII SalI SbfI ScaI SfiI
SfoI SfuI SgrAI SpeI SphI
Sse8387I SspBI SwaI XhoI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
Acc65I 5374 GGTACC G^GTACC
AccII 742 CGCG CG^CG
AcyI 18 GACGCC G[GA]^CG[CT]C
AflII 2254 CTTAAG C^TTAAG
Alw44I 1557 GTGCAC G^TGCAC
Aor51HI 2899 AGCGCT AGC^GCT
ApaI 609 GGGCCC GGGCC^C
ApaLI 1557 GTGCAC G^TGCAC
Asp700I 2316 GAAGTGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 5374 GGTACC G^GTACC
BclI 1502 TGATCA T^GATCA
BfrI 2254 CTTAAG C^TTAAG
BsaHI 18 GACGCC G[GA]^CG[CT]C
BsaWI 74 ACCGGA [AT]^CCGG[AT]
BsiEI 3366 CGGCCG CG[GA][CT]^CG
BsmBI 1368 GAGACG CGTCTC(1/5)
BsrBI 359 GAGCGG CCGCTC(-3/-3)
BstUI 742 CGCG CG^CG
DrdI 4459 GACTGGGGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 3366 CGGCCG C^GGCCG
EclXI 3366 CGGCCG C^GGCCG
Eco47III 2899 AGCGCT AGC^GCT
Eco52I 3366 CGGCCG C^GGCCG
EcoNI 1603 CCTGCAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I 2736 ATGCAT ATGCA^T
FbaI 1502 TGATCA T^GATCA
HaeII 2899 AGCGCT [GA]GCGC^[CT]
Hin1I 18 GACGCC G[GA]^CG[CT]C
HindIII 2001 AAGCTT A^AGCTT
KpnI 5374 GGTACC GGTAC^C
MvnI 742 CGCG CG^CG
NaeI 3633 GCCGGC GCC^GGC
NcoI 5145 CCATGG C^CATGG
NdeI 3962 CATATG CA^TATG
NgoMIV 3633 GCCGGC G^CCGGC
NheI 2902 GCTAGC G^CTAGC
NsiI 2736 ATGCAT ATGCA^T
PacI 3496 TTAATTAA TTAAT^TAA
PflMI 5645 CCAAGACATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SacI 4293 GAGCTC GAGCT^C
SexAI 2120 ACCAGGT A^CC[AT]GGT
SnaBI 312 TACGTA TAC^GTA
StuI 4665 AGGCCT AGG^CCT
Van91I 5645 CCAAGACATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 2316 GAAGTGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AocI 265 CCTCAGG CC^T[ACGT]AGG
1414 CCTCAGG
AseI 2433 ATTAAT AT^TAAT
3499 ATTAAT
AsnI 2433 ATTAAT AT^TAAT
3499 ATTAAT
AspI 1203 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
3749 GACAGAGTC
BcgI 778 CGATCCTCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
179 GCACATGTTTCG
BglII 3877 AGATCT A^GATCT
5019 AGATCT
BsmI 4265 GAATGC GAATGC(1/-1)
3557 GCATTC
BspLU11I 181 ACATGT A^CATGT
3378 ACATGT
BstAPI 1260 GCAACCTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3401 GCAGGACATGC
Bsu36I 265 CCTCAGG CC^T[ACGT]AGG
1414 CCTCAGG
DraIII 199 CACTGTGTG CAC[ACGT][ACGT][ACGT]^GTG
5056 CACCACGTG
Eco81I 265 CCTCAGG CC^T[ACGT]AGG
1414 CCTCAGG
EcoRI 3334 GAATTC G^AATTC
5119 GAATTC
HpaI 3289 GTTAAC GTT^AAC
5132 GTTAAC
PflFI 1203 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
3749 GACAGAGTC
PpuMI 2039 AGGACCC [GA]G^G[AT]CC[CT]
5164 AGGTCCT
PshBI 2433 ATTAAT AT^TAAT
3499 ATTAAT
SmaI 612 CCCGGG CCC^GGG
1273 CCCGGG
TaqI 1402 TCGA T^CGA
3894 TCGA
Tth111I 1203 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
3749 GACAGAGTC
TthHB8I 1402 TCGA T^CGA
3894 TCGA
XmaCI 612 CCCGGG C^CCGGG
1273 CCCGGG
XmaI 612 CCCGGG C^CCGGG
1273 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 121 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI AlwNI ApoI AspHI AvaI
AvaII BalI BanI BanII BbrPI
BbsI BbvCI BbvI BcnI BfaI
BglI BlpI BmyI BpmI Bpu1102I
BpuAI BsaAI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrDI
BsrFI BsrI BssKI BstF5I BstNI
BstXI BstYI Cac8I CelII CfoI
Cfr10I Cfr13I DdeI DpnII DraI
DraII DsaI EaeI EarI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HgaI HhaI HinP1I
HincII HindII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV NspI PleI PmaCI PmlI
PstI PvuII RsaI SapI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
SspI StyI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI XbaI XcmI
XhoII