##################################
# NO CUT ENZYMES ( 67 enzymes) #
##################################
Acc65I AclI AflII AgeI Aor51HI
AscI AseI AsnI Asp718I AspI
AviII BciVI BfrI BsiWI BspDI
BspHI BsrDI BssHII Bst1107I BstBI
BstEII BstPI BstZ17I ClaI CpoI
Eco47III EcoO65I EcoRV EcoT22I FseI
FspI HincII HindII HpaI KpnI
MfeI MluI MunI NdeI NotI
NruI NsiI NspV PacI PflFI
PinAI PmeI PshAI PshBI Psp1406I
PvuI RcaI RsrII SalI SbfI
ScaI SexAI SfiI SfuI SgrAI
SnaBI SpeI Sse8387I SspI SwaI
Tth111I XbaI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
AatII 4886 GACGTC GACGT^C
AccIII 1070 TCCGGA T^CCGGA
AflIII 3227 ACATGT A^C[GA][CT]GT
AhdI 117 GACTTGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 117 GACTTGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI 2992 GGATCC G^GATCC
BbeI 72 GGCGCC GGCGC^C
BbrPI 2044 CACGTG CAC^GTG
BbvCI 2492 CCTCAGC CCTCAGC(-5/-2)
BcgI 3353 GCAGCTTCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 1816 TGATCA T^GATCA
BsaAI 2044 CACGTG [CT]AC^GT[GA]
BsaBI 2643 GATGCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 1070 TCCGGA T^CCGGA
Bsp1407I 551 TGTACA T^GTACA
BspEI 1070 TCCGGA T^CCGGA
BspLU11I 3227 ACATGT A^CATGT
BsrGI 551 TGTACA T^GTACA
BstAPI 2969 GCACCCACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
DraI 4380 TTTAAA TTT^AAA
Eam1105I 117 GACTTGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FbaI 1816 TGATCA T^GATCA
HaeII 72 GGCGCC [GA]GCGC^[CT]
HindIII 1457 AAGCTT A^AGCTT
KasI 72 GGCGCC G^GCGCC
KspI 2435 CCGCGG CCGC^GG
MamI 2643 GATGCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 1070 TCCGGA T^CCGGA
MseI 4381 TTAA T^TAA
NarI 72 GGCGCC GG^CGCC
NheI 53 GCTAGC G^CTAGC
PaeR7I 1051 CTCGAG C^TCGAG
PmaCI 2044 CACGTG CAC^GTG
PmlI 2044 CACGTG CAC^GTG
SacII 2435 CCGCGG CCGC^GG
SfoI 72 GGCGCC GGC^GCC
SphI 2825 GCATGC GCATG^C
SspBI 551 TGTACA T^GTACA
TfiI 313 GATTC G^A[AT]TC
Tru9I 4381 TTAA T^TAA
XhoI 1051 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AccI 125 GTCTAC GT^[AC][GT]AC
438 GTCTAC
Alw44I 3146 GTGCAC G^TGCAC
3391 GTGCAC
AlwNI 2653 CAGGGCCTG CAG[ACGT][ACGT][ACGT]^CTG
3969 CAGGGTCTG
ApaLI 3146 GTGCAC G^TGCAC
3391 GTGCAC
Asp700I 3000 GAACTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3567 GAACTGCTTC
AvrII 3812 CCTAGG C^CTAGG
4150 CCTAGG
BlnI 3812 CCTAGG C^CTAGG
4150 CCTAGG
BlpI 468 GCTGAGC GC^T[ACGT]AGC
2290 GCTGAGC
Bpu1102I 468 GCTGAGC GC^T[ACGT]AGC
2290 GCTGAGC
BsaWI 1070 TCCGGA [AT]^CCGG[AT]
3116 ACCGGA
BsmBI 4261 CGTCTC CGTCTC(1/5)
4888 CGTCTC
BsmI 1432 GAATGC GAATGC(1/-1)
3328 GAATGC
BssSI 3184 CACGAG CACGAG(-5/-1)
3400 CACGAG
CelII 468 GCTGAGC GC^T[ACGT]AGC
2290 GCTGAGC
DraIII 3149 CACAGTGTG CAC[ACGT][ACGT][ACGT]^GTG
3294 CACGCTGTG
DrdI 4010 GACCTAATTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4880 GACTCTGACGTC
EagI 2693 CGGCCG C^GGCCG
3447 CGGCCG
EclXI 2693 CGGCCG C^GGCCG
3447 CGGCCG
Eco52I 2693 CGGCCG C^GGCCG
3447 CGGCCG
EcoRI 4323 GAATTC G^AATTC
4682 GAATTC
NspI 2825 GCATGC [GA]CATG^[CT]
3227 ACATGT
XmnI 3000 GAACTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3567 GAACTGCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 128 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI AocI ApaI ApoI
AspHI AvaI AvaII BalI BanI
BanII BbsI BbvI BcnI BfaI
BglI BglII BmyI BpmI BpuAI
BsaHI BsaI BsaJI BseRI BsgI
BsiEI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrBI
BsrFI BsrI BssKI BstF5I BstNI
BstUI BstXI BstYI Bsu36I Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI EarI Eco81I
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MslI MspA1I MspI
MvaI MvnI MwoI NaeI NciI
NcoI NdeII NgoMIV NlaIII NlaIV
PflMI PleI PpuMI PstI PvuII
RsaI SacI SapI Sau3AI Sau96I
ScrFI SfaNI SfcI SmaI SmlI
StuI StyI TaqI TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XcmI
XhoII XmaCI XmaI