##################################
# NO CUT ENZYMES ( 56 enzymes) #
##################################
AclI AflII AflIII AgeI Alw44I
ApaLI AseI AsnI AspI AviII
BbeI BciVI BfrI BsiWI BspLU11I
Bst1107I BstBI BstZ17I CpoI DraIII
EcoRI EcoT22I FseI FspI KasI
MfeI MluI MunI NarI NheI
NotI NruI NsiI NspV PacI
PflFI PinAI PmeI PshBI Psp1406I
PvuI RsrII SacI SalI SapI
SexAI SfiI SfoI SfuI SgrAI
SnaBI SpeI SspI SwaI Tth111I
XbaI
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
AatII 1370 GACGTC GACGT^C
Acc65I 2560 GGTACC G^GTACC
AccI 3039 GTCTAC GT^[AC][GT]AC
AccIII 2038 TCCGGA T^CCGGA
AscI 112 GGCGCGCC GG^CGCGCC
Asp718I 2560 GGTACC G^GTACC
AvrII 442 CCTAGG C^CTAGG
BcgI 1620 CGAGTGGCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 2131 TGATCA T^GATCA
BglII 65 AGATCT A^GATCT
BlnI 442 CCTAGG C^CTAGG
BseAI 2038 TCCGGA T^CCGGA
BsiEI 455 CGGCCG CG[GA][CT]^CG
BspDI 4977 ATCGAT AT^CGAT
BspEI 2038 TCCGGA T^CCGGA
BsrBI 4558 GAGCGG CCGCTC(-3/-3)
BsrDI 5619 GCAATG GCAATG(2/0)
ClaI 4977 ATCGAT AT^CGAT
EagI 455 CGGCCG C^GGCCG
EclXI 455 CGGCCG C^GGCCG
Eco52I 455 CGGCCG C^GGCCG
EcoRV 5692 GATATC GAT^ATC
FbaI 2131 TGATCA T^GATCA
HpaI 4608 GTTAAC GTT^AAC
KpnI 2560 GGTACC GGTAC^C
KspI 222 CCGCGG CCGC^GG
MroI 2038 TCCGGA T^CCGGA
NaeI 453 GCCGGC GCC^GGC
NcoI 872 CCATGG C^CATGG
NdeI 5674 CATATG CA^TATG
NgoMIV 453 GCCGGC G^CCGGC
PaeR7I 1618 CTCGAG C^TCGAG
PshAI 2217 GACAACAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacII 222 CCGCGG CCGC^GG
SmaI 4490 CCCGGG CCC^GGG
SphI 4635 GCATGC GCATG^C
XhoI 1618 CTCGAG C^TCGAG
XmaCI 4490 CCCGGG C^CCGGG
XmaI 4490 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 36 enzymes) #
###################################
AcyI 389 GGCGTC G[GA]^CG[CT]C
1370 GACGTC
Aor51HI 795 AGCGCT AGC^GCT
4962 AGCGCT
ApaI 2293 GGGCCC GGGCC^C
2713 GGGCCC
Asp700I 1175 GAACCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2205 GAATCATTTC
BamHI 1462 GGATCC G^GATCC
5441 GGATCC
BbrPI 405 CACGTG CAC^GTG
5095 CACGTG
BlpI 1569 GCTCAGC GC^T[ACGT]AGC
2510 GCTCAGC
Bpu1102I 1569 GCTCAGC GC^T[ACGT]AGC
2510 GCTCAGC
BsaAI 405 CACGTG [CT]AC^GT[GA]
5095 CACGTG
BsaBI 2200 GATTTGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
5704 GATGTCTATC
BsaHI 389 GGCGTC G[GA]^CG[CT]C
1370 GACGTC
BsaWI 2038 TCCGGA [AT]^CCGG[AT]
3542 TCCGGT
BsmBI 1372 CGTCTC CGTCTC(1/5)
3738 GAGACG
BspHI 4448 TCATGA T^CATGA
5416 TCATGA
BssHII 113 GCGCGC G^CGCGC
410 GCGCGC
BstEII 686 GGTAACC G^GT[ACGT]ACC
1781 GGTGACC
BstPI 686 GGTAACC G^GT[ACGT]ACC
1781 GGTGACC
CelII 1569 GCTCAGC GC^T[ACGT]AGC
2510 GCTCAGC
DraI 5541 TTTAAA TTT^AAA
5735 TTTAAA
DrdI 1364 GACATGGACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2726 GACCGTGGTGTC
EarI 5103 CTCTTC CTCTTC(1/4)
1764 GAAGAG
Eco47III 795 AGCGCT AGC^GCT
4962 AGCGCT
EcoO65I 686 GGTAACC G^GT[ACGT]ACC
1781 GGTGACC
Hin1I 389 GGCGTC G[GA]^CG[CT]C
1370 GACGTC
HindIII 1489 AAGCTT A^AGCTT
5686 AAGCTT
Ksp632I 5103 CTCTTC CTCTTC(1/4)
1764 GAAGAG
MamI 2200 GATTTGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
5704 GATGTCTATC
NspI 2901 GCATGT [GA]CATG^[CT]
4635 GCATGC
PmaCI 405 CACGTG CAC^GTG
5095 CACGTG
PmlI 405 CACGTG CAC^GTG
5095 CACGTG
RcaI 4448 TCATGA T^CATGA
5416 TCATGA
SbfI 1019 CCTGCAGG CCTGCA^GG
1112 CCTGCAGG
ScaI 896 AGTACT AGT^ACT
1933 AGTACT
SmlI 1618 CTCGAG C^T[CT][GA]AG
4739 CTCAAG
Sse8387I 1019 CCTGCAGG CCTGCA^GG
1112 CCTGCAGG
XmnI 1175 GAACCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2205 GAATCATTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 127 enzymes) #
#########################################
AccII AciI AcsI AfaI AhdI
AluI AlwI AlwNI AocI ApoI
AspEI AspHI AvaI AvaII BalI
BanI BanII BbsI BbvCI BbvI
BcnI BfaI BglI BmyI BpmI
BpuAI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI BsoBI Bsp1286I Bsp1407I BspMI
BsrFI BsrGI BsrI BssKI BssSI
BstAPI BstF5I BstNI BstUI BstXI
BstYI Bsu36I Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI Eam1105I Eco81I EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
HinP1I HincII HindII HinfI HpaII
HphI ItaI MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV PflMI PleI PpuMI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SspBI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XcmI XhoII