##################################
# NO CUT ENZYMES ( 59 enzymes) #
##################################
AatII Acc65I AclI AcyI AgeI
Aor51HI AscI Asp700I Asp718I AspI
AviII BbeI BsaHI BsiEI BsiWI
BsmI BspDI BsrBI Bst1107I BstAPI
BstZ17I ClaI CpoI DraI EagI
EclXI Eco47III Eco52I EcoT22I FseI
FspI Hin1I HpaI KasI KpnI
KspI MluI NarI NotI NruI
NsiI PacI PflFI PinAI PmeI
Psp1406I PvuI RsrII SacII SalI
SexAI SfoI SgrAI SnaBI SpeI
SphI SwaI Tth111I XmnI
###################################
# ONE CUT ENZYMES ( 53 enzymes) #
###################################
AflIII 2254 ACATGT A^C[GA][CT]GT
Alw44I 1400 GTGCAC G^TGCAC
ApaI 5671 GGGCCC GGGCC^C
ApaLI 1400 GTGCAC G^TGCAC
AseI 3272 ATTAAT AT^TAAT
AsnI 3272 ATTAAT AT^TAAT
AvrII 3493 CCTAGG C^CTAGG
BamHI 2105 GGATCC G^GATCC
BbrPI 4558 CACGTG CAC^GTG
BciVI 1855 GGATAC GTATCC(6/5)
BclI 5041 TGATCA T^GATCA
BlnI 3493 CCTAGG C^CTAGG
BlpI 616 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 616 GCTCAGC GC^T[ACGT]AGC
BsaAI 4558 CACGTG [CT]AC^GT[GA]
Bsp1407I 2257 TGTACA T^GTACA
BspHI 1690 TCATGA T^CATGA
BspLU11I 2254 ACATGT A^CATGT
BspMI 4897 GCAGGT ACCTGC(4/8)
BsrGI 2257 TGTACA T^GTACA
BstBI 598 TTCGAA TT^CGAA
BstEII 3792 GGTCACC G^GT[ACGT]ACC
BstPI 3792 GGTCACC G^GT[ACGT]ACC
CelII 616 GCTCAGC GC^T[ACGT]AGC
DrdI 5378 GACAAAGGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 311 CCATGG C^C[GA][CT]GG
EcoO65I 3792 GGTCACC G^GT[ACGT]ACC
EcoRI 1021 GAATTC G^AATTC
EcoRV 970 GATATC GAT^ATC
FbaI 5041 TGATCA T^GATCA
HincII 3916 GTTGAC GT[CT]^[GA]AC
HindII 3916 GTTGAC GT[CT]^[GA]AC
HindIII 4047 AAGCTT A^AGCTT
NaeI 1572 GCCGGC GCC^GGC
NcoI 311 CCATGG C^CATGG
NdeI 1793 CATATG CA^TATG
NgoMIV 1572 GCCGGC G^CCGGC
NheI 4995 GCTAGC G^CTAGC
NspV 598 TTCGAA TT^CGAA
PaeR7I 2083 CTCGAG C^TCGAG
PmaCI 4558 CACGTG CAC^GTG
PmlI 4558 CACGTG CAC^GTG
PshBI 3272 ATTAAT AT^TAAT
RcaI 1690 TCATGA T^CATGA
SapI 594 GCTCTTC GCTCTTC(1/4)
SbfI 1162 CCTGCAGG CCTGCA^GG
ScaI 3 AGTACT AGT^ACT
SfiI 4930 GGCCAAAGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfuI 598 TTCGAA TT^CGAA
Sse8387I 1162 CCTGCAGG CCTGCA^GG
SspBI 2257 TGTACA T^GTACA
SspI 140 AATATT AAT^ATT
XhoI 2083 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AccI 364 GTAGAC GT^[AC][GT]AC
466 GTAGAC
AccIII 200 TCCGGA T^CCGGA
5285 TCCGGA
AflII 1121 CTTAAG C^TTAAG
2445 CTTAAG
AhdI 3471 GACATGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4654 GACCCTGTGTC
AspEI 3471 GACATGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4654 GACCCTGTGTC
BbsI 660 GAAGAC GAAGAC(2/6)
4412 GTCTTC
BcgI 225 CGAACTAAATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1702 GCAGCTGCCTCG
BfrI 1121 CTTAAG C^TTAAG
2445 CTTAAG
BglII 1734 AGATCT A^GATCT
4964 AGATCT
BpuAI 660 GAAGAC GAAGAC(2/6)
4412 GTCTTC
BsaBI 966 GATTGATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2211 GATGAGAATC
BsaWI 200 TCCGGA [AT]^CCGG[AT]
5285 TCCGGA
BseAI 200 TCCGGA T^CCGGA
5285 TCCGGA
BspEI 200 TCCGGA T^CCGGA
5285 TCCGGA
BsrDI 612 CATTGC GCAATG(2/0)
4733 CATTGC
BsrFI 1572 GCCGGC [GA]^CCGG[CT]
4491 GCCGGT
BssHII 5619 GCGCGC G^CGCGC
5621 GCGCGC
BssSI 2851 CACGAG CACGAG(-5/-1)
5037 CTCGTG
Cfr10I 1572 GCCGGC [GA]^CCGG[CT]
4491 GCCGGT
DraIII 34 CACGCAGTG CAC[ACGT][ACGT][ACGT]^GTG
1282 CACCTTGTG
Eam1105I 3471 GACATGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4654 GACCCTGTGTC
MamI 966 GATTGATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2211 GATGAGAATC
MfeI 405 CAATTG C^AATTG
4636 CAATTG
MroI 200 TCCGGA T^CCGGA
5285 TCCGGA
MunI 405 CAATTG C^AATTG
4636 CAATTG
NspI 795 ACATGC [GA]CATG^[CT]
2254 ACATGT
PshAI 2244 GACAGTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
3411 GACTACAGTC
SmaI 674 CCCGGG CCC^GGG
5674 CCCGGG
XbaI 1265 TCTAGA T^CTAGA
1919 TCTAGA
XmaCI 674 CCCGGG C^CCGGG
5674 CCCGGG
XmaI 674 CCCGGG C^CCGGG
5674 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 115 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI AlwNI AocI ApoI AspHI
AvaI AvaII BalI BanI BanII
BbvCI BbvI BcnI BfaI BglI
BmyI BpmI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmBI BsmFI BsoBI Bsp1286I BsrI
BssKI BstF5I BstNI BstUI BstXI
BstYI Bsu36I Cac8I CfoI Cfr13I
DdeI DpnII DraII EaeI EarI
Eco81I EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV PflMI
PleI PpuMI PstI PvuII RsaI
SacI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XcmI XhoII