################################## # NO CUT ENZYMES ( 59 enzymes) # ################################## AatII Acc65I AclI AcyI AgeI Aor51HI AscI Asp700I Asp718I AspI AviII BbeI BsaHI BsiEI BsiWI BsmI BspDI BsrBI Bst1107I BstAPI BstZ17I ClaI CpoI DraI EagI EclXI Eco47III Eco52I EcoT22I FseI FspI Hin1I HpaI KasI KpnI KspI MluI NarI NotI NruI NsiI PacI PflFI PinAI PmeI Psp1406I PvuI RsrII SacII SalI SexAI SfoI SgrAI SnaBI SpeI SphI SwaI Tth111I XmnI ################################### # ONE CUT ENZYMES ( 53 enzymes) # ################################### AflIII 2254 ACATGT A^C[GA][CT]GT Alw44I 1400 GTGCAC G^TGCAC ApaI 5671 GGGCCC GGGCC^C ApaLI 1400 GTGCAC G^TGCAC AseI 3272 ATTAAT AT^TAAT AsnI 3272 ATTAAT AT^TAAT AvrII 3493 CCTAGG C^CTAGG BamHI 2105 GGATCC G^GATCC BbrPI 4558 CACGTG CAC^GTG BciVI 1855 GGATAC GTATCC(6/5) BclI 5041 TGATCA T^GATCA BlnI 3493 CCTAGG C^CTAGG BlpI 616 GCTCAGC GC^T[ACGT]AGC Bpu1102I 616 GCTCAGC GC^T[ACGT]AGC BsaAI 4558 CACGTG [CT]AC^GT[GA] Bsp1407I 2257 TGTACA T^GTACA BspHI 1690 TCATGA T^CATGA BspLU11I 2254 ACATGT A^CATGT BspMI 4897 GCAGGT ACCTGC(4/8) BsrGI 2257 TGTACA T^GTACA BstBI 598 TTCGAA TT^CGAA BstEII 3792 GGTCACC G^GT[ACGT]ACC BstPI 3792 GGTCACC G^GT[ACGT]ACC CelII 616 GCTCAGC GC^T[ACGT]AGC DrdI 5378 GACAAAGGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC DsaI 311 CCATGG C^C[GA][CT]GG EcoO65I 3792 GGTCACC G^GT[ACGT]ACC EcoRI 1021 GAATTC G^AATTC EcoRV 970 GATATC GAT^ATC FbaI 5041 TGATCA T^GATCA HincII 3916 GTTGAC GT[CT]^[GA]AC HindII 3916 GTTGAC GT[CT]^[GA]AC HindIII 4047 AAGCTT A^AGCTT NaeI 1572 GCCGGC GCC^GGC NcoI 311 CCATGG C^CATGG NdeI 1793 CATATG CA^TATG NgoMIV 1572 GCCGGC G^CCGGC NheI 4995 GCTAGC G^CTAGC NspV 598 TTCGAA TT^CGAA PaeR7I 2083 CTCGAG C^TCGAG PmaCI 4558 CACGTG CAC^GTG PmlI 4558 CACGTG CAC^GTG PshBI 3272 ATTAAT AT^TAAT RcaI 1690 TCATGA T^CATGA SapI 594 GCTCTTC GCTCTTC(1/4) SbfI 1162 CCTGCAGG CCTGCA^GG ScaI 3 AGTACT AGT^ACT SfiI 4930 GGCCAAAGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SfuI 598 TTCGAA TT^CGAA Sse8387I 1162 CCTGCAGG CCTGCA^GG SspBI 2257 TGTACA T^GTACA SspI 140 AATATT AAT^ATT XhoI 2083 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 31 enzymes) # ################################### AccI 364 GTAGAC GT^[AC][GT]AC 466 GTAGAC AccIII 200 TCCGGA T^CCGGA 5285 TCCGGA AflII 1121 CTTAAG C^TTAAG 2445 CTTAAG AhdI 3471 GACATGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 4654 GACCCTGTGTC AspEI 3471 GACATGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 4654 GACCCTGTGTC BbsI 660 GAAGAC GAAGAC(2/6) 4412 GTCTTC BcgI 225 CGAACTAAATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 1702 GCAGCTGCCTCG BfrI 1121 CTTAAG C^TTAAG 2445 CTTAAG BglII 1734 AGATCT A^GATCT 4964 AGATCT BpuAI 660 GAAGAC GAAGAC(2/6) 4412 GTCTTC BsaBI 966 GATTGATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 2211 GATGAGAATC BsaWI 200 TCCGGA [AT]^CCGG[AT] 5285 TCCGGA BseAI 200 TCCGGA T^CCGGA 5285 TCCGGA BspEI 200 TCCGGA T^CCGGA 5285 TCCGGA BsrDI 612 CATTGC GCAATG(2/0) 4733 CATTGC BsrFI 1572 GCCGGC [GA]^CCGG[CT] 4491 GCCGGT BssHII 5619 GCGCGC G^CGCGC 5621 GCGCGC BssSI 2851 CACGAG CACGAG(-5/-1) 5037 CTCGTG Cfr10I 1572 GCCGGC [GA]^CCGG[CT] 4491 GCCGGT DraIII 34 CACGCAGTG CAC[ACGT][ACGT][ACGT]^GTG 1282 CACCTTGTG Eam1105I 3471 GACATGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 4654 GACCCTGTGTC MamI 966 GATTGATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 2211 GATGAGAATC MfeI 405 CAATTG C^AATTG 4636 CAATTG MroI 200 TCCGGA T^CCGGA 5285 TCCGGA MunI 405 CAATTG C^AATTG 4636 CAATTG NspI 795 ACATGC [GA]CATG^[CT] 2254 ACATGT PshAI 2244 GACAGTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 3411 GACTACAGTC SmaI 674 CCCGGG CCC^GGG 5674 CCCGGG XbaI 1265 TCTAGA T^CTAGA 1919 TCTAGA XmaCI 674 CCCGGG C^CCGGG 5674 CCCGGG XmaI 674 CCCGGG C^CCGGG 5674 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 115 enzymes) # ######################################### AccII AciI AcsI AfaI AluI AlwI AlwNI AocI ApoI AspHI AvaI AvaII BalI BanI BanII BbvCI BbvI BcnI BfaI BglI BmyI BpmI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmBI BsmFI BsoBI Bsp1286I BsrI BssKI BstF5I BstNI BstUI BstXI BstYI Bsu36I Cac8I CfoI Cfr13I DdeI DpnII DraII EaeI EarI Eco81I EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV PflMI PleI PpuMI PstI PvuII RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI XhoII