##################################
# NO CUT ENZYMES ( 74 enzymes) #
##################################
AatII Acc65I AclI AflII AgeI
AhdI Aor51HI AscI AseI AsnI
Asp700I Asp718I AspEI AviII BbeI
BciVI BfrI BsaBI BsiWI BspDI
BspHI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstZ17I ClaI CpoI
EagI Eam1105I EclXI Eco47III Eco52I
EcoO65I EcoRV FseI FspI HaeII
HincII HindII HindIII HpaI KasI
KpnI KspI MamI MfeI MluI
MunI NarI NheI NotI NruI
NspV PacI PinAI PmeI PshBI
Psp1406I PvuI RcaI RsrII SacII
SalI SexAI SfoI SfuI SgrAI
SnaBI SwaI XbaI XmnI
###################################
# ONE CUT ENZYMES ( 31 enzymes) #
###################################
AccIII 1337 TCCGGA T^CCGGA
AvrII 1156 CCTAGG C^CTAGG
BcgI 2258 GCAGGAGAATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 1696 TGATCA T^GATCA
BlnI 1156 CCTAGG C^CTAGG
BlpI 903 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 903 GCTAAGC GC^T[ACGT]AGC
BsaWI 1337 TCCGGA [AT]^CCGG[AT]
BseAI 1337 TCCGGA T^CCGGA
BsiEI 1366 CGGTCG CG[GA][CT]^CG
BspEI 1337 TCCGGA T^CCGGA
BsrBI 3371 GAGCGG CCGCTC(-3/-3)
BstAPI 1569 GCAACCTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CelII 903 GCTAAGC GC^T[ACGT]AGC
DrdI 4628 GACTAACTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 2411 GAATTC G^AATTC
EcoT22I 686 ATGCAT ATGCA^T
FbaI 1696 TGATCA T^GATCA
MroI 1337 TCCGGA T^CCGGA
NaeI 517 GCCGGC GCC^GGC
NdeI 3762 CATATG CA^TATG
NgoMIV 517 GCCGGC G^CCGGC
NsiI 686 ATGCAT ATGCA^T
PaeR7I 5189 CTCGAG C^TCGAG
PshAI 4823 GACACATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 895 GAGCTC GAGCT^C
ScaI 4127 AGTACT AGT^ACT
SpeI 482 ACTAGT A^CTAGT
SphI 5194 GCATGC GCATG^C
SspI 679 AATATT AAT^ATT
XhoI 5189 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AccI 4419 GTCTAC GT^[AC][GT]AC
5286 GTCTAC
AccII 2771 CGCG CG^CG
2842 CGCG
AflIII 4298 ACATGT A^C[GA][CT]GT
4826 ACATGT
Alw44I 1635 GTGCAC G^TGCAC
3913 GTGCAC
ApaLI 1635 GTGCAC G^TGCAC
3913 GTGCAC
AspI 1403 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1512 GACGGAGTC
BamHI 3830 GGATCC G^GATCC
3902 GGATCC
BglII 848 AGATCT A^GATCT
1481 AGATCT
BsmI 3304 GAATGC GAATGC(1/-1)
3936 GCATTC
Bsp1407I 2548 TGTACA T^GTACA
4271 TGTACA
BspLU11I 4298 ACATGT A^CATGT
4826 ACATGT
BsrFI 517 GCCGGC [GA]^CCGG[CT]
5214 ACCGGC
BsrGI 2548 TGTACA T^GTACA
4271 TGTACA
BstUI 2771 CGCG CG^CG
2842 CGCG
Cfr10I 517 GCCGGC [GA]^CCGG[CT]
5214 ACCGGC
MvnI 2771 CGCG CG^CG
2842 CGCG
NcoI 3543 CCATGG C^CATGG
5639 CCATGG
PflFI 1403 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1512 GACGGAGTC
PvuII 3505 CAGCTG CAG^CTG
3820 CAGCTG
SapI 863 GCTCTTC GCTCTTC(1/4)
5541 GCTCTTC
SbfI 278 CCTGCAGG CCTGCA^GG
4891 CCTGCAGG
SfiI 2531 GGCCTTGAAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
4652 GGCCCAGAGGGCC
Sse8387I 278 CCTGCAGG CCTGCA^GG
4891 CCTGCAGG
SspBI 2548 TGTACA T^GTACA
4271 TGTACA
StuI 2530 AGGCCT AGG^CCT
3270 AGGCCT
Tth111I 1403 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1512 GACGGAGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 127 enzymes) #
#########################################
AciI AcsI AcyI AfaI AluI
AlwI AlwNI AocI ApaI ApoI
AspHI AvaI AvaII BalI BanI
BanII BbrPI BbsI BbvCI BbvI
BcnI BfaI BglI BmyI BpmI
BpuAI BsaAI BsaHI BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmBI BsmFI BsoBI Bsp1286I
BspMI BsrDI BsrI BssKI BstF5I
BstNI BstXI BstYI Bsu36I Cac8I
CfoI Cfr13I DdeI DpnII DraI
DraII DraIII DsaI EaeI EarI
Eco81I EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV NspI PflMI
PleI PmaCI PmlI PpuMI PstI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmaI SmlI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XcmI XhoII
XmaCI XmaI