##################################
# NO CUT ENZYMES ( 129 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AgeI AhdI Aor51HI ApaI AscI
Asp718I AspEI AspI AvaI AvaII
AviII AvrII BalI BanI BbeI
BbrPI BbvCI BcgI BciVI BfaI
BglI BlnI BlpI BpmI Bpu1102I
BsaBI BsaWI BseAI BseRI BsiWI
BsmBI BsmI BsoBI Bsp1407I BspDI
BspEI BspHI BsrDI BsrFI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstZ17I CelII Cfr10I ClaI CpoI
DraII DraIII DrdI Eam1105I Eco47III
EcoNI EcoO109I EcoRI EcoRV EcoT14I
EcoT22I FseI FspI HaeII HpaI
KasI KpnI MaeI MamI MfeI
MluI MluNI MroI MscI MunI
NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PpuMI Psp1406I PvuI
PvuII RcaI RsrII SacI SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SpeI
SphI Sse8387I SspBI StuI StyI
SwaI TaqI Tth111I TthHB8I XbaI
XcmI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 50 enzymes) #
###################################
AflII 759 CTTAAG C^TTAAG
AflIII 318 ACATGT A^C[GA][CT]GT
Alw44I 522 GTGCAC G^TGCAC
AlwNI 610 CAGACTCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 599 CCTCAGG CC^T[ACGT]AGG
ApaLI 522 GTGCAC G^TGCAC
AseI 1268 ATTAAT AT^TAAT
AsnI 1268 ATTAAT AT^TAAT
Asp700I 31 GAACGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 522 GTGCAC G[AT]GC[AT]^C
BanII 969 GAGCCC G[GA]GC[CT]^C
BbsI 962 GTCTTC GAAGAC(2/6)
BfrI 759 CTTAAG C^TTAAG
BglII 1013 AGATCT A^GATCT
BpuAI 962 GTCTTC GAAGAC(2/6)
BsaAI 1356 TACGTA [CT]AC^GT[GA]
BsaI 566 GGTCTC GGTCTC(1/5)
BsgI 662 CTGCAC GTGCAG(16/14)
BsiEI 43 CGGCCG CG[GA][CT]^CG
BsiHKAI 522 GTGCAC G[AT]GC[AT]^C
BspLU11I 318 ACATGT A^CATGT
BspMI 158 ACCTGC ACCTGC(4/8)
BsrBI 98 GAGCGG CCGCTC(-3/-3)
BstEII 720 GGTGACC G^GT[ACGT]ACC
BstPI 720 GGTGACC G^GT[ACGT]ACC
Bsu36I 599 CCTCAGG CC^T[ACGT]AGG
DraI 1249 TTTAAA TTT^AAA
EaeI 43 CGGCCG [CT]^GGCC[GA]
EagI 43 CGGCCG C^GGCCG
EarI 908 CTCTTC CTCTTC(1/4)
EclXI 43 CGGCCG C^GGCCG
Eco52I 43 CGGCCG C^GGCCG
Eco81I 599 CCTCAGG CC^T[ACGT]AGG
EcoO65I 720 GGTGACC G^GT[ACGT]ACC
HincII 733 GTTGAC GT[CT]^[GA]AC
HindII 733 GTTGAC GT[CT]^[GA]AC
HindIII 264 AAGCTT A^AGCTT
Ksp632I 908 CTCTTC CTCTTC(1/4)
KspI 40 CCGCGG CCGC^GG
MaeII 1357 ACGT A^CGT
MspA1I 40 CCGCGG C[AC]G^C[GT]G
NspI 318 ACATGT [GA]CATG^[CT]
PflMI 502 CCAACTGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 955 GACTTCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 1268 ATTAAT AT^TAAT
SacII 40 CCGCGG CCGC^GG
SnaBI 1356 TACGTA TAC^GTA
SspI 225 AATATT AAT^ATT
Van91I 502 CCAACTGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 31 GAACGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 14 enzymes) #
###################################
AcyI 117 GACGCC G[GA]^CG[CT]C
125 GACGCC
AfaI 156 GTAC GT^AC
769 GTAC
BamHI 231 GGATCC G^GATCC
498 GGATCC
BclI 606 TGATCA T^GATCA
1444 TGATCA
BmyI 522 GTGCAC G[AGT]GC[ACT]^C
969 GAGCCC
BsaHI 117 GACGCC G[GA]^CG[CT]C
125 GACGCC
Bsp1286I 522 GTGCAC G[AGT]GC[ACT]^C
969 GAGCCC
BstXI 573 CCACATCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
974 CCACTCAGGTGG
DsaI 40 CCGCGG C^C[GA][CT]GG
129 CCACGG
FbaI 606 TGATCA T^GATCA
1444 TGATCA
HgaI 117 GACGC GACGC(5/10)
125 GACGC
Hin1I 117 GACGCC G[GA]^CG[CT]C
125 GACGCC
PstI 352 CTGCAG CTGCA^G
377 CTGCAG
RsaI 156 GTAC GT^AC
769 GTAC
#########################################
# 3 OR MORE CUT ENZYMES ( 65 enzymes) #
#########################################
AccII AciI AcsI AluI AlwI
ApoI BbvI BcnI BsaJI BsiYI
BslI BsmAI BsmFI BsrI BssKI
BstF5I BstNI BstUI BstYI Cac8I
CfoI Cfr13I DdeI DpnII EcoRII
FauI Fnu4HI FokI HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI MaeIII MboI MboII MflI
MnlI MseI MslI MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV PleI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI XhoII