##################################
# NO CUT ENZYMES ( 48 enzymes) #
##################################
AatII AgeI AhdI Alw44I ApaLI
AscI AseI AsnI AspEI AviII
AvrII BclI BlnI BsiWI BspDI
BspHI Bst1107I BstZ17I ClaI CpoI
DrdI Eam1105I EcoNI EcoRI FbaI
FseI FspI HpaI MluI NdeI
NotI NruI PacI PinAI PmeI
PshBI RcaI RsrII SacI SalI
SbfI SgrAI SnaBI SpeI SphI
Sse8387I SspI SwaI
###################################
# ONE CUT ENZYMES ( 34 enzymes) #
###################################
AccI 3508 GTCTAC GT^[AC][GT]AC
AccIII 1709 TCCGGA T^CCGGA
AclI 3514 AACGTT AA^CGTT
AflII 3046 CTTAAG C^TTAAG
ApaI 2763 GGGCCC GGGCC^C
BciVI 2562 GGATAC GTATCC(6/5)
BfrI 3046 CTTAAG C^TTAAG
BglII 4901 AGATCT A^GATCT
BseAI 1709 TCCGGA T^CCGGA
BsmBI 3051 GAGACG CGTCTC(1/5)
Bsp1407I 4978 TGTACA T^GTACA
BspEI 1709 TCCGGA T^CCGGA
BsrDI 5133 GCAATG GCAATG(2/0)
BsrGI 4978 TGTACA T^GTACA
BssHII 709 GCGCGC G^CGCGC
BstAPI 1071 GCACTATGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 5292 TTCGAA TT^CGAA
DraI 5300 TTTAAA TTT^AAA
EagI 1560 CGGCCG C^GGCCG
EclXI 1560 CGGCCG C^GGCCG
Eco52I 1560 CGGCCG C^GGCCG
HindIII 1570 AAGCTT A^AGCTT
MfeI 5334 CAATTG C^AATTG
MroI 1709 TCCGGA T^CCGGA
MunI 5334 CAATTG C^AATTG
NheI 3984 GCTAGC G^CTAGC
NspV 5292 TTCGAA TT^CGAA
Psp1406I 3514 AACGTT AA^CGTT
PvuI 2622 CGATCG CGAT^CG
ScaI 2231 AGTACT AGT^ACT
SfiI 3473 GGCCTGGTCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfuI 5292 TTCGAA TT^CGAA
SspBI 4978 TGTACA T^GTACA
XbaI 5270 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AocI 762 CCTGAGG CC^T[ACGT]AGG
2598 CCTGAGG
AspI 565 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1019 GACCAGGTC
BalI 536 TGGCCA TGG^CCA
3702 TGGCCA
BbrPI 1507 CACGTG CAC^GTG
3225 CACGTG
BglI 773 GCCTCTATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
3474 GCCTGGTCGGC
BsaBI 481 GATCCGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1182 GATGTTCATC
BsmI 2238 GAATGC GAATGC(1/-1)
1786 GCATTC
BspLU11I 1891 ACATGT A^CATGT
1922 ACATGT
Bsu36I 762 CCTGAGG CC^T[ACGT]AGG
2598 CCTGAGG
Eco81I 762 CCTGAGG CC^T[ACGT]AGG
2598 CCTGAGG
EcoRV 2128 GATATC GAT^ATC
3644 GATATC
EcoT22I 286 ATGCAT ATGCA^T
2240 ATGCAT
KspI 317 CCGCGG CCGC^GG
1242 CCGCGG
MamI 481 GATCCGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1182 GATGTTCATC
MluNI 536 TGGCCA TGG^CCA
3702 TGGCCA
MscI 536 TGGCCA TGG^CCA
3702 TGGCCA
NaeI 1853 GCCGGC GCC^GGC
3368 GCCGGC
NgoMIV 1853 GCCGGC G^CCGGC
3368 GCCGGC
NsiI 286 ATGCAT ATGCA^T
2240 ATGCAT
PflFI 565 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1019 GACCAGGTC
PmaCI 1507 CACGTG CAC^GTG
3225 CACGTG
PmlI 1507 CACGTG CAC^GTG
3225 CACGTG
PshAI 3852 GACAGGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
4711 GACATAGGTC
SacII 317 CCGCGG CCGC^GG
1242 CCGCGG
SexAI 1020 ACCAGGT A^CC[AT]GGT
2909 ACCTGGT
Tth111I 565 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1019 GACCAGGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 150 enzymes) #
#########################################
Acc65I AccII AciI AcsI AcyI
AfaI AflIII AluI AlwI AlwNI
Aor51HI ApoI Asp700I Asp718I AspHI
AvaI AvaII BamHI BanI BanII
BbeI BbsI BbvCI BbvI BcgI
BcnI BfaI BlpI BmyI BpmI
Bpu1102I BpuAI BsaAI BsaHI BsaI
BsaJI BsaWI BseRI BsgI BsiEI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrBI BsrFI
BsrI BssKI BssSI BstEII BstF5I
BstNI BstPI BstUI BstXI BstYI
Cac8I CelII CfoI Cfr10I Cfr13I
DdeI DpnII DraII DraIII DsaI
EaeI EarI Eco47III EcoO109I EcoO65I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HincII HindII HinfI
HpaII HphI ItaI KasI KpnI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NarI NciI NcoI NdeII NlaIII
NlaIV NspI PaeR7I PflMI PleI
PpuMI PstI PvuII RsaI SapI
Sau3AI Sau96I ScrFI SfaNI SfcI
SfoI SmaI SmlI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XcmI
XhoI XhoII XmaCI XmaI XmnI