##################################
# NO CUT ENZYMES ( 69 enzymes) #
##################################
AatII Acc65I AclI AgeI Aor51HI
AscI Asp718I AviII AvrII BbrPI
BbvCI BglI BlnI BsiEI BsiWI
BsmBI Bsp1407I BspDI BsrFI BsrGI
BssHII BssSI Bst1107I BstZ17I Cfr10I
ClaI CpoI EagI EclXI Eco47III
Eco52I EcoT22I FseI FspI HindIII
HpaI KpnI KspI MluI MspA1I
NaeI NgoMIV NotI NruI NsiI
PacI PaeR7I PflMI PinAI PmaCI
PmeI PmlI Psp1406I PvuI PvuII
RsrII SacII SalI SbfI SexAI
SfiI SgrAI SpeI SphI Sse8387I
SspBI SwaI Van91I XhoI
###################################
# ONE CUT ENZYMES ( 54 enzymes) #
###################################
AccI 1568 GTCTAC GT^[AC][GT]AC
AcyI 2664 GGCGCC G[GA]^CG[CT]C
AflII 2195 CTTAAG C^TTAAG
AflIII 1303 ACATGT A^C[GA][CT]GT
Alw44I 3350 GTGCAC G^TGCAC
AocI 4048 CCTAAGG CC^T[ACGT]AGG
ApaLI 3350 GTGCAC G^TGCAC
AseI 5198 ATTAAT AT^TAAT
AsnI 5198 ATTAAT AT^TAAT
Asp700I 4520 GAAGTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 1065 GACTAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BanI 2664 GGCGCC G^G[CT][GA]CC
BbeI 2664 GGCGCC GGCGC^C
BcgI 3342 CGATGGTTGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 2195 CTTAAG C^TTAAG
BglII 742 AGATCT A^GATCT
BlpI 4267 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 4267 GCTAAGC GC^T[ACGT]AGC
BsaHI 2664 GGCGCC G[GA]^CG[CT]C
BspLU11I 1303 ACATGT A^CATGT
BspMI 2080 ACCTGC ACCTGC(4/8)
BstBI 3183 TTCGAA TT^CGAA
BstEII 702 GGTCACC G^GT[ACGT]ACC
BstPI 702 GGTCACC G^GT[ACGT]ACC
Bsu36I 4048 CCTAAGG CC^T[ACGT]AGG
CelII 4267 GCTAAGC GC^T[ACGT]AGC
DraIII 840 CACTGGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1436 GACATACAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 4048 CCTAAGG CC^T[ACGT]AGG
EcoNI 3107 CCTAGCAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 702 GGTCACC G^GT[ACGT]ACC
HgaI 3646 GCGTC GACGC(5/10)
Hin1I 2664 GGCGCC G[GA]^CG[CT]C
KasI 2664 GGCGCC G^GCGCC
NarI 2664 GGCGCC GG^CGCC
NheI 2693 GCTAGC G^CTAGC
NspV 3183 TTCGAA TT^CGAA
PflFI 1065 GACTAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 2500 AGGTCCT [GA]G^G[AT]CC[CT]
PshAI 28 GACAGGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 5198 ATTAAT AT^TAAT
SacI 3632 GAGCTC GAGCT^C
SapI 1244 GAAGAGC GCTCTTC(1/4)
ScaI 3999 AGTACT AGT^ACT
SfoI 2664 GGCGCC GGC^GCC
SfuI 3183 TTCGAA TT^CGAA
SmaI 2883 CCCGGG CCC^GGG
SspI 3728 AATATT AAT^ATT
StuI 1693 AGGCCT AGG^CCT
Tth111I 1065 GACTAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 2594 TCTAGA T^CTAGA
XmaCI 2883 CCCGGG C^CCGGG
XmaI 2883 CCCGGG C^CCGGG
XmnI 4520 GAAGTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AccIII 385 TCCGGA T^CCGGA
4145 TCCGGA
ApaI 319 GGGCCC GGGCC^C
3854 GGGCCC
AvaI 1180 CCCGAG C^[CT]CG[GA]G
2883 CCCGGG
BciVI 3818 GGATAC GTATCC(6/5)
254 GTATCC
BpmI 894 CTCCAG CTGGAG(16/14)
4251 CTGGAG
BsaAI 2010 TACGTA [CT]AC^GT[GA]
3303 TACGTA
BsaBI 2447 GATCTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4370 GATCAATATC
BsaWI 385 TCCGGA [AT]^CCGG[AT]
4145 TCCGGA
BseAI 385 TCCGGA T^CCGGA
4145 TCCGGA
BseRI 486 GAGGAG GAGGAG(10/8)
1592 GAGGAG
BsmI 2932 GAATGC GAATGC(1/-1)
4787 GAATGC
BsoBI 1180 CCCGAG C^[CT]CG[GA]G
2883 CCCGGG
BspEI 385 TCCGGA T^CCGGA
4145 TCCGGA
BspHI 652 TCATGA T^CATGA
3054 TCATGA
BsrBI 573 CCGCTC CCGCTC(-3/-3)
2265 CCGCTC
BstAPI 1988 GCAAAGACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
4129 GCAAGGAGTGC
BstXI 309 CCATTGACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1215 CCACCATTTTGG
EcoRV 2203 GATATC GAT^ATC
4310 GATATC
HaeII 1165 AGCGCC [GA]GCGC^[CT]
2664 GGCGCC
MamI 2447 GATCTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4370 GATCAATATC
MfeI 1410 CAATTG C^AATTG
2787 CAATTG
MroI 385 TCCGGA T^CCGGA
4145 TCCGGA
MunI 1410 CAATTG C^AATTG
2787 CAATTG
NdeI 2871 CATATG CA^TATG
3738 CATATG
RcaI 652 TCATGA T^CATGA
3054 TCATGA
SnaBI 2010 TACGTA TAC^GTA
3303 TACGTA
XcmI 392 CCAGAAATCACTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1554 CCAACTATGAACTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 108 enzymes) #
#########################################
AccII AciI AcsI AfaI AhdI
AluI AlwI AlwNI ApoI AspEI
AspHI AvaII BalI BamHI BanII
BbsI BbvI BclI BcnI BfaI
BmyI BpuAI BsaI BsaJI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I BsrDI BsrI BssKI BstF5I
BstNI BstUI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraI DraII
DsaI EaeI Eam1105I EarI EcoO109I
EcoRI EcoRII EcoT14I FauI FbaI
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HincII HindII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspI
MvaI MvnI MwoI NciI NcoI
NdeII NlaIII NlaIV NspI PleI
PstI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII