##################################
# NO CUT ENZYMES ( 57 enzymes) #
##################################
AatII Acc65I AflII AgeI AhdI
AscI Asp718I AspEI AspI AvrII
BfrI BlnI BspDI BsrFI BssHII
Cfr10I ClaI CpoI DraI Eam1105I
EcoRI FseI HpaI KpnI KspI
MluI NaeI NdeI NgoMIV NheI
NotI NruI PacI PflFI PflMI
PinAI PmeI PshAI PvuI RsrII
SacII SalI SbfI ScaI SexAI
SfiI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SwaI Tth111I Van91I
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 35 enzymes) #
###################################
AccIII 5294 TCCGGA T^CCGGA
AocI 3179 CCTGAGG CC^T[ACGT]AGG
ApaI 3807 GGGCCC GGGCC^C
AseI 3641 ATTAAT AT^TAAT
AsnI 3641 ATTAAT AT^TAAT
BbeI 5687 GGCGCC GGCGC^C
BbrPI 1178 CACGTG CAC^GTG
BseAI 5294 TCCGGA T^CCGGA
BsiWI 2648 CGTACG C^GTACG
BspEI 5294 TCCGGA T^CCGGA
BspHI 4806 TCATGA T^CATGA
BssSI 1574 CTCGTG CACGAG(-5/-1)
BstBI 3486 TTCGAA TT^CGAA
BstEII 3563 GGTTACC G^GT[ACGT]ACC
BstPI 3563 GGTTACC G^GT[ACGT]ACC
Bsu36I 3179 CCTGAGG CC^T[ACGT]AGG
DrdI 15 GACAGCGCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 3179 CCTGAGG CC^T[ACGT]AGG
EcoO65I 3563 GGTTACC G^GT[ACGT]ACC
EcoRV 3833 GATATC GAT^ATC
HindIII 5819 AAGCTT A^AGCTT
KasI 5687 GGCGCC G^GCGCC
MroI 5294 TCCGGA T^CCGGA
NarI 5687 GGCGCC GG^CGCC
NspV 3486 TTCGAA TT^CGAA
PaeR7I 2791 CTCGAG C^TCGAG
PmaCI 1178 CACGTG CAC^GTG
PmlI 1178 CACGTG CAC^GTG
PshBI 3641 ATTAAT AT^TAAT
RcaI 4806 TCATGA T^CATGA
SfoI 5687 GGCGCC GGC^GCC
SfuI 3486 TTCGAA TT^CGAA
SmlI 2791 CTCGAG C^T[CT][GA]AG
StuI 2817 AGGCCT AGG^CCT
XhoI 2791 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 37 enzymes) #
###################################
AccI 2963 GTATAC GT^[AC][GT]AC
4420 GTATAC
AclI 915 AACGTT AA^CGTT
2678 AACGTT
Alw44I 545 GTGCAC G^TGCAC
2786 GTGCAC
ApaLI 545 GTGCAC G^TGCAC
2786 GTGCAC
AviII 118 TGCGCA TGC^GCA
3555 TGCGCA
BamHI 2700 GGATCC G^GATCC
4567 GGATCC
BbvCI 1993 CCTCAGC CCTCAGC(-5/-2)
2627 CCTCAGC
BglI 283 GCCTCTGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2507 GCCCCCATGGC
BglII 1708 AGATCT A^GATCT
3663 AGATCT
BsaAI 1178 CACGTG [CT]AC^GT[GA]
5219 TACGTG
BsaBI 1258 GATACAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3664 GATCTCAATC
BsiEI 438 CGGCCG CG[GA][CT]^CG
2861 CGGCCG
BsmBI 2826 CGTCTC CGTCTC(1/5)
395 GAGACG
BsmI 1010 GAATGC GAATGC(1/-1)
1918 GAATGC
Bsp1407I 2838 TGTACA T^GTACA
3303 TGTACA
BsrBI 1167 CCGCTC CCGCTC(-3/-3)
4592 GAGCGG
BsrGI 2838 TGTACA T^GTACA
3303 TGTACA
Bst1107I 2963 GTATAC GTA^TAC
4420 GTATAC
BstZ17I 2963 GTATAC GTA^TAC
4420 GTATAC
DraIII 5633 CACTTTGTG CAC[ACGT][ACGT][ACGT]^GTG
5651 CACCCAGTG
EagI 438 CGGCCG C^GGCCG
2861 CGGCCG
EclXI 438 CGGCCG C^GGCCG
2861 CGGCCG
Eco52I 438 CGGCCG C^GGCCG
2861 CGGCCG
EcoNI 684 CCTGCTATAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
4262 CCTACCAAAGG
EcoT22I 2115 ATGCAT ATGCA^T
3850 ATGCAT
FspI 118 TGCGCA TGC^GCA
3555 TGCGCA
HincII 1548 GTCAAC GT[CT]^[GA]AC
3707 GTTGAC
HindII 1548 GTCAAC GT[CT]^[GA]AC
3707 GTTGAC
MamI 1258 GATACAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3664 GATCTCAATC
MfeI 5535 CAATTG C^AATTG
5927 CAATTG
MunI 5535 CAATTG C^AATTG
5927 CAATTG
NsiI 2115 ATGCAT ATGCA^T
3850 ATGCAT
Psp1406I 915 AACGTT AA^CGTT
2678 AACGTT
SapI 222 GAAGAGC GCTCTTC(1/4)
1469 GAAGAGC
SspBI 2838 TGTACA T^GTACA
3303 TGTACA
SspI 2135 AATATT AAT^ATT
5243 AATATT
XbaI 2450 TCTAGA T^CTAGA
5227 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 129 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflIII AluI AlwI AlwNI Aor51HI
ApoI Asp700I AspHI AvaI AvaII
BalI BanI BanII BbsI BbvI
BcgI BciVI BclI BcnI BfaI
BlpI BmyI BpmI Bpu1102I BpuAI
BsaHI BsaI BsaJI BsaWI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspLU11I BspMI
BsrDI BsrI BssKI BstAPI BstF5I
BstNI BstUI BstXI BstYI Cac8I
CelII CfoI Cfr13I DdeI DpnII
DraII DsaI EaeI EarI Eco47III
EcoO109I EcoRII EcoT14I FauI FbaI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NcoI NdeII NlaIII NlaIV
NspI PleI PpuMI PstI PvuII
RsaI SacI Sau3AI Sau96I ScrFI
SfaNI SfcI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XcmI XhoII XmnI