##################################
# NO CUT ENZYMES ( 86 enzymes) #
##################################
Acc65I AccI AccIII AclI AflII
Alw44I Aor51HI ApaLI AscI Asp718I
AspI AviII BalI BamHI BbrPI
BbvCI BfrI BglI BlpI Bpu1102I
BsaAI BseAI BsiEI BsiWI BspEI
BsrBI BssHII Bst1107I BstBI BstEII
BstPI BstXI BstZ17I CelII CpoI
EagI EclXI Eco47III Eco52I EcoO65I
FseI FspI HincII HindII HpaI
KpnI MfeI MluI MluNI MroI
MscI MunI NaeI NcoI NgoMIV
NotI NruI NspV PacI PflFI
PflMI PmaCI PmeI PmlI PshAI
Psp1406I PvuI RsrII SacI SalI
SbfI ScaI SexAI SfiI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SwaI Tth111I Van91I XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
AatII 3939 GACGTC GACGT^C
AhdI 5355 GACAATCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 2358 CCTGAGG CC^T[ACGT]AGG
ApaI 162 GGGCCC GGGCC^C
AspEI 5355 GACAATCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 3775 GTGCTC G[AT]GC[AT]^C
AvrII 2481 CCTAGG C^CTAGG
BbeI 384 GGCGCC GGCGC^C
BcgI 3944 CGATACAGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlnI 2481 CCTAGG C^CTAGG
BsaBI 2390 GATCAGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 4207 GAGACC GGTCTC(1/5)
BsiHKAI 3775 GTGCTC G[AT]GC[AT]^C
Bsp1407I 4307 TGTACA T^GTACA
BspDI 3838 ATCGAT AT^CGAT
BspHI 3557 TCATGA T^CATGA
BsrGI 4307 TGTACA T^GTACA
BssSI 243 CTCGTG CACGAG(-5/-1)
Bsu36I 2358 CCTGAGG CC^T[ACGT]AGG
ClaI 3838 ATCGAT AT^CGAT
DraIII 2293 CACACTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 2957 GACTATTGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 264 CCGCGG C^C[GA][CT]GG
EaeI 340 CGGCCA [CT]^GGCC[GA]
Eam1105I 5355 GACAATCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 2358 CCTGAGG CC^T[ACGT]AGG
HindIII 3822 AAGCTT A^AGCTT
KasI 384 GGCGCC G^GCGCC
KspI 264 CCGCGG CCGC^GG
MamI 2390 GATCAGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NarI 384 GGCGCC GG^CGCC
NheI 1049 GCTAGC G^CTAGC
PaeR7I 3778 CTCGAG C^TCGAG
RcaI 3557 TCATGA T^CATGA
SacII 264 CCGCGG CCGC^GG
SapI 349 GCTCTTC GCTCTTC(1/4)
SfoI 384 GGCGCC GGC^GCC
SspBI 4307 TGTACA T^GTACA
XcmI 1130 CCAAGACACATCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 3778 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AflIII 3354 ACATGT A^C[GA][CT]GT
4123 ACATGT
AgeI 585 ACCGGT A^CCGGT
655 ACCGGT
AseI 2764 ATTAAT AT^TAAT
2832 ATTAAT
AsnI 2764 ATTAAT AT^TAAT
2832 ATTAAT
Asp700I 4434 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
5741 GAAATCCTTC
BciVI 4533 GGATAC GTATCC(6/5)
2065 GTATCC
BclI 1713 TGATCA T^GATCA
5250 TGATCA
BsgI 2091 CTGCAC GTGCAG(16/14)
5176 CTGCAC
BsmBI 116 GAGACG CGTCTC(1/5)
4769 GAGACG
BsmI 1385 GAATGC GAATGC(1/-1)
2527 GAATGC
BspLU11I 3354 ACATGT A^CATGT
4123 ACATGT
BsrDI 523 CATTGC GCAATG(2/0)
3579 CATTGC
BsrFI 585 ACCGGT [GA]^CCGG[CT]
655 ACCGGT
BstAPI 823 GCAGAGGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2208 GCATCAGTTGC
Cfr10I 585 ACCGGT [GA]^CCGG[CT]
655 ACCGGT
EcoNI 203 CCTCGGTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
5175 CCTGCACAAGG
EcoRI 2434 GAATTC G^AATTC
3853 GAATTC
EcoRV 186 GATATC GAT^ATC
3599 GATATC
EcoT22I 2447 ATGCAT ATGCA^T
5512 ATGCAT
FbaI 1713 TGATCA T^GATCA
5250 TGATCA
NdeI 1751 CATATG CA^TATG
4611 CATATG
NsiI 2447 ATGCAT ATGCA^T
5512 ATGCAT
PinAI 585 ACCGGT A^CCGGT
655 ACCGGT
PshBI 2764 ATTAAT AT^TAAT
2832 ATTAAT
PstI 1448 CTGCAG CTGCA^G
4048 CTGCAG
XmnI 4434 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
5741 GAAATCCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 106 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI AlwNI ApoI AvaI
AvaII BanI BanII BbsI BbvI
BcnI BfaI BglII BmyI BpmI
BpuAI BsaHI BsaJI BsaWI BseRI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrI BssKI BstF5I
BstNI BstUI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraI DraII
EarI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV NspI PleI PpuMI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI SspI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XbaI
XhoII