################################## # NO CUT ENZYMES ( 86 enzymes) # ################################## Acc65I AccI AccIII AclI AflII Alw44I Aor51HI ApaLI AscI Asp718I AspI AviII BalI BamHI BbrPI BbvCI BfrI BglI BlpI Bpu1102I BsaAI BseAI BsiEI BsiWI BspEI BsrBI BssHII Bst1107I BstBI BstEII BstPI BstXI BstZ17I CelII CpoI EagI EclXI Eco47III Eco52I EcoO65I FseI FspI HincII HindII HpaI KpnI MfeI MluI MluNI MroI MscI MunI NaeI NcoI NgoMIV NotI NruI NspV PacI PflFI PflMI PmaCI PmeI PmlI PshAI Psp1406I PvuI RsrII SacI SalI SbfI ScaI SexAI SfiI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SwaI Tth111I Van91I XmaCI XmaI ################################### # ONE CUT ENZYMES ( 40 enzymes) # ################################### AatII 3939 GACGTC GACGT^C AhdI 5355 GACAATCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AocI 2358 CCTGAGG CC^T[ACGT]AGG ApaI 162 GGGCCC GGGCC^C AspEI 5355 GACAATCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspHI 3775 GTGCTC G[AT]GC[AT]^C AvrII 2481 CCTAGG C^CTAGG BbeI 384 GGCGCC GGCGC^C BcgI 3944 CGATACAGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BlnI 2481 CCTAGG C^CTAGG BsaBI 2390 GATCAGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaI 4207 GAGACC GGTCTC(1/5) BsiHKAI 3775 GTGCTC G[AT]GC[AT]^C Bsp1407I 4307 TGTACA T^GTACA BspDI 3838 ATCGAT AT^CGAT BspHI 3557 TCATGA T^CATGA BsrGI 4307 TGTACA T^GTACA BssSI 243 CTCGTG CACGAG(-5/-1) Bsu36I 2358 CCTGAGG CC^T[ACGT]AGG ClaI 3838 ATCGAT AT^CGAT DraIII 2293 CACACTGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 2957 GACTATTGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC DsaI 264 CCGCGG C^C[GA][CT]GG EaeI 340 CGGCCA [CT]^GGCC[GA] Eam1105I 5355 GACAATCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco81I 2358 CCTGAGG CC^T[ACGT]AGG HindIII 3822 AAGCTT A^AGCTT KasI 384 GGCGCC G^GCGCC KspI 264 CCGCGG CCGC^GG MamI 2390 GATCAGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC NarI 384 GGCGCC GG^CGCC NheI 1049 GCTAGC G^CTAGC PaeR7I 3778 CTCGAG C^TCGAG RcaI 3557 TCATGA T^CATGA SacII 264 CCGCGG CCGC^GG SapI 349 GCTCTTC GCTCTTC(1/4) SfoI 384 GGCGCC GGC^GCC SspBI 4307 TGTACA T^GTACA XcmI 1130 CCAAGACACATCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoI 3778 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### AflIII 3354 ACATGT A^C[GA][CT]GT 4123 ACATGT AgeI 585 ACCGGT A^CCGGT 655 ACCGGT AseI 2764 ATTAAT AT^TAAT 2832 ATTAAT AsnI 2764 ATTAAT AT^TAAT 2832 ATTAAT Asp700I 4434 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 5741 GAAATCCTTC BciVI 4533 GGATAC GTATCC(6/5) 2065 GTATCC BclI 1713 TGATCA T^GATCA 5250 TGATCA BsgI 2091 CTGCAC GTGCAG(16/14) 5176 CTGCAC BsmBI 116 GAGACG CGTCTC(1/5) 4769 GAGACG BsmI 1385 GAATGC GAATGC(1/-1) 2527 GAATGC BspLU11I 3354 ACATGT A^CATGT 4123 ACATGT BsrDI 523 CATTGC GCAATG(2/0) 3579 CATTGC BsrFI 585 ACCGGT [GA]^CCGG[CT] 655 ACCGGT BstAPI 823 GCAGAGGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 2208 GCATCAGTTGC Cfr10I 585 ACCGGT [GA]^CCGG[CT] 655 ACCGGT EcoNI 203 CCTCGGTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 5175 CCTGCACAAGG EcoRI 2434 GAATTC G^AATTC 3853 GAATTC EcoRV 186 GATATC GAT^ATC 3599 GATATC EcoT22I 2447 ATGCAT ATGCA^T 5512 ATGCAT FbaI 1713 TGATCA T^GATCA 5250 TGATCA NdeI 1751 CATATG CA^TATG 4611 CATATG NsiI 2447 ATGCAT ATGCA^T 5512 ATGCAT PinAI 585 ACCGGT A^CCGGT 655 ACCGGT PshBI 2764 ATTAAT AT^TAAT 2832 ATTAAT PstI 1448 CTGCAG CTGCA^G 4048 CTGCAG XmnI 4434 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 5741 GAAATCCTTC ######################################### # 3 OR MORE CUT ENZYMES ( 106 enzymes) # ######################################### AccII AciI AcsI AcyI AfaI AluI AlwI AlwNI ApoI AvaI AvaII BanI BanII BbsI BbvI BcnI BfaI BglII BmyI BpmI BpuAI BsaHI BsaJI BsaWI BseRI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrI BssKI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraI DraII EarI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NspI PleI PpuMI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SspI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XbaI XhoII