##################################
# NO CUT ENZYMES ( 76 enzymes) #
##################################
Acc65I AccII AccIII AclI AgeI
AscI Asp718I AviII AvrII BamHI
BcnI BlnI BlpI Bpu1102I BsaWI
BseAI BsiWI BspDI BspEI BsrBI
BsrFI BssHII Bst1107I BstBI BstUI
BstZ17I CelII Cfr10I ClaI CpoI
EagI EclXI Eco52I EcoNI EcoRV
FseI FspI HapII HpaII KpnI
KspI MfeI MluI MroI MspI
MunI MvnI NaeI NciI NdeI
NgoMIV NheI NotI NruI NspV
PacI PinAI PmeI PshAI Psp1406I
PvuI RsrII SacII SalI SbfI
SexAI SfiI SfuI SgrAI SmaI
SnaBI SphI Sse8387I StuI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 54 enzymes) #
###################################
AatII 3012 GACGTC GACGT^C
AccI 1639 GTAGAC GT^[AC][GT]AC
AflII 2143 CTTAAG C^TTAAG
AhdI 4717 GACTGTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 24 GTGCAC G^TGCAC
AocI 2101 CCTCAGG CC^T[ACGT]AGG
Aor51HI 3936 AGCGCT AGC^GCT
ApaI 3850 GGGCCC GGGCC^C
ApaLI 24 GTGCAC G^TGCAC
Asp700I 4393 GAAGGGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 4717 GACTGTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 844 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 951 CTCGAG C^[CT]CG[GA]G
BbeI 971 GGCGCC GGCGC^C
BbsI 3219 GAAGAC GAAGAC(2/6)
BbvCI 3330 CCTCAGC CCTCAGC(-5/-2)
BcgI 931 CGATTGTTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 3595 GGATAC GTATCC(6/5)
BclI 2673 TGATCA T^GATCA
BfrI 2143 CTTAAG C^TTAAG
BglI 4612 GCCTTAGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1327 AGATCT A^GATCT
BpuAI 3219 GAAGAC GAAGAC(2/6)
BsaBI 1558 GATTGGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI 3977 CGACCG CG[GA][CT]^CG
BsmBI 842 GAGACG CGTCTC(1/5)
BsoBI 951 CTCGAG C^[CT]CG[GA]G
Bsp1407I 1489 TGTACA T^GTACA
BspHI 1622 TCATGA T^CATGA
BsrGI 1489 TGTACA T^GTACA
BstEII 4313 GGTGACC G^GT[ACGT]ACC
BstPI 4313 GGTGACC G^GT[ACGT]ACC
Bsu36I 2101 CCTCAGG CC^T[ACGT]AGG
Eam1105I 4717 GACTGTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 3936 AGCGCT AGC^GCT
Eco81I 2101 CCTCAGG CC^T[ACGT]AGG
EcoO65I 4313 GGTGACC G^GT[ACGT]ACC
FauI 71 GCGGG CCCGC(4/6)
FbaI 2673 TGATCA T^GATCA
KasI 971 GGCGCC G^GCGCC
MamI 1558 GATTGGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NarI 971 GGCGCC GG^CGCC
PaeR7I 951 CTCGAG C^TCGAG
PflFI 844 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 3539 CCAAGATATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RcaI 1622 TCATGA T^CATGA
SapI 664 GAAGAGC GCTCTTC(1/4)
SfoI 971 GGCGCC GGC^GCC
SspBI 1489 TGTACA T^GTACA
SwaI 1130 ATTTAAAT ATTT^AAAT
Tth111I 844 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 3539 CCAAGATATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 951 CTCGAG C^TCGAG
XmnI 4393 GAAGGGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 16 enzymes) #
###################################
BalI 1021 TGGCCA TGG^CCA
1112 TGGCCA
BsmI 2319 GAATGC GAATGC(1/-1)
3198 GAATGC
BssSI 3791 CACGAG CACGAG(-5/-1)
3814 CACGAG
DraIII 63 CACGTGGTG CAC[ACGT][ACGT][ACGT]^GTG
1846 CACAAGGTG
DrdI 1238 GACTCAGCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1816 GACCAAGGAGTC
EaeI 1021 TGGCCA [CT]^GGCC[GA]
1112 TGGCCA
EarI 664 GAAGAG CTCTTC(1/4)
5023 GAAGAG
EcoRI 3325 GAATTC G^AATTC
4846 GAATTC
HgaI 2581 GACGC GACGC(5/10)
3733 GCGTC
Ksp632I 664 GAAGAG CTCTTC(1/4)
5023 GAAGAG
MluNI 1021 TGGCCA TGG^CCA
1112 TGGCCA
MscI 1021 TGGCCA TGG^CCA
1112 TGGCCA
PstI 875 CTGCAG CTGCA^G
1973 CTGCAG
ScaI 1462 AGTACT AGT^ACT
2861 AGTACT
SpeI 434 ACTAGT A^CTAGT
4521 ACTAGT
XbaI 1859 TCTAGA T^CTAGA
2619 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 112 enzymes) #
#########################################
AciI AcsI AcyI AfaI AflIII
AluI AlwI AlwNI ApoI AseI
AsnI AspHI AvaII BanI BanII
BbrPI BbvI BfaI BmyI BpmI
BsaAI BsaHI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BspLU11I BspMI BsrDI
BsrI BssKI BstAPI BstF5I BstNI
BstXI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraI DraII DsaI
EcoO109I EcoRII EcoT14I EcoT22I Fnu4HI
FokI HaeII HaeIII HhaI Hin1I
HinP1I HincII HindII HindIII HinfI
HpaI HphI ItaI MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MvaI MwoI
NcoI NdeII NlaIII NlaIV NsiI
NspI PleI PmaCI PmlI PpuMI
PshBI PvuII RsaI SacI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
SspI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XcmI XhoII