##################################
# NO CUT ENZYMES ( 65 enzymes) #
##################################
AccI AccIII AgeI AhdI Aor51HI
AscI AseI AsnI AspEI BbeI
BbvCI BsaBI BseAI BsiWI BsmBI
BspDI BspEI BspHI BsrBI BssHII
Bst1107I BstBI BstXI BstZ17I ClaI
CpoI EagI Eam1105I EclXI Eco47III
Eco52I FseI HaeII HgaI HpaI
KasI KspI MamI MluI MroI
NaeI NarI NgoMIV NheI NotI
NruI NspV PacI PflMI PinAI
PmeI PshBI PvuI RcaI RsrII
SacII SalI ScaI SfoI SfuI
SgrAI SnaBI StuI SwaI Van91I
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AatII 1819 GACGTC GACGT^C
AccII 1318 CGCG CG^CG
AclI 5226 AACGTT AA^CGTT
AcyI 1819 GACGTC G[GA]^CG[CT]C
AflII 4772 CTTAAG C^TTAAG
Alw44I 1207 GTGCAC G^TGCAC
ApaLI 1207 GTGCAC G^TGCAC
Asp700I 4987 GAAATTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 2344 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 3982 TGCGCA TGC^GCA
AvrII 2930 CCTAGG C^CTAGG
BcgI 1147 CGAGGGGAATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 4772 CTTAAG C^TTAAG
BlnI 2930 CCTAGG C^CTAGG
BlpI 1468 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1468 GCTCAGC GC^T[ACGT]AGC
BsaHI 1819 GACGTC G[GA]^CG[CT]C
BsaWI 4459 TCCGGT [AT]^CCGG[AT]
BsiEI 3100 CGACCG CG[GA][CT]^CG
Bsp1407I 5536 TGTACA T^GTACA
BsrDI 3295 GCAATG GCAATG(2/0)
BsrFI 533 ACCGGC [GA]^CCGG[CT]
BsrGI 5536 TGTACA T^GTACA
BstUI 1318 CGCG CG^CG
CelII 1468 GCTCAGC GC^T[ACGT]AGC
Cfr10I 533 ACCGGC [GA]^CCGG[CT]
DsaI 1129 CCATGG C^C[GA][CT]GG
FspI 3982 TGCGCA TGC^GCA
Hin1I 1819 GACGTC G[GA]^CG[CT]C
HincII 640 GTCAAC GT[CT]^[GA]AC
HindII 640 GTCAAC GT[CT]^[GA]AC
HindIII 4035 AAGCTT A^AGCTT
MfeI 3721 CAATTG C^AATTG
MunI 3721 CAATTG C^AATTG
MvnI 1318 CGCG CG^CG
NcoI 1129 CCATGG C^CATGG
NdeI 5499 CATATG CA^TATG
PflFI 2344 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
Psp1406I 5226 AACGTT AA^CGTT
SapI 49 GAAGAGC GCTCTTC(1/4)
SbfI 3360 CCTGCAGG CCTGCA^GG
SpeI 5413 ACTAGT A^CTAGT
Sse8387I 3360 CCTGCAGG CCTGCA^GG
SspBI 5536 TGTACA T^GTACA
Tth111I 2344 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmnI 4987 GAAATTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
Acc65I 1449 GGTACC G^GTACC
3303 GGTACC
Asp718I 1449 GGTACC G^GTACC
3303 GGTACC
BalI 269 TGGCCA TGG^CCA
3815 TGGCCA
BamHI 1811 GGATCC G^GATCC
2781 GGATCC
BbrPI 895 CACGTG CAC^GTG
4019 CACGTG
BciVI 1252 GGATAC GTATCC(6/5)
4365 GGATAC
BsgI 257 CTGCAC GTGCAG(16/14)
832 GTGCAG
BstAPI 19 GCAGAGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2031 GCAGGAAATGC
DraIII 1183 CACAGGGTG CAC[ACGT][ACGT][ACGT]^GTG
1624 CACCCGGTG
EarI 5526 CTCTTC CTCTTC(1/4)
49 GAAGAG
EcoNI 2193 CCTGGAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
4080 CCTAACCAAGG
EcoRI 1522 GAATTC G^AATTC
4599 GAATTC
EcoT22I 5162 ATGCAT ATGCA^T
6128 ATGCAT
KpnI 1449 GGTACC GGTAC^C
3303 GGTACC
Ksp632I 5526 CTCTTC CTCTTC(1/4)
49 GAAGAG
MluNI 269 TGGCCA TGG^CCA
3815 TGGCCA
MscI 269 TGGCCA TGG^CCA
3815 TGGCCA
MslI 2985 CATGGGCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
3852 CACTATGATG
NsiI 5162 ATGCAT ATGCA^T
6128 ATGCAT
PaeR7I 2596 CTCGAG C^TCGAG
2641 CTCGAG
PmaCI 895 CACGTG CAC^GTG
4019 CACGTG
PmlI 895 CACGTG CAC^GTG
4019 CACGTG
PshAI 667 GACATCCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
3549 GACAGAGGTC
PstI 3361 CTGCAG CTGCA^G
5060 CTGCAG
XbaI 5578 TCTAGA T^CTAGA
5698 TCTAGA
XhoI 2596 CTCGAG C^TCGAG
2641 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 121 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI AlwNI AocI ApaI ApoI
AspHI AvaI AvaII BanI BanII
BbsI BbvI BclI BcnI BfaI
BglI BglII BmyI BpmI BpuAI
BsaAI BsaI BsaJI BseRI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
BsoBI Bsp1286I BspLU11I BspMI BsrI
BssKI BssSI BstEII BstF5I BstNI
BstPI BstYI Bsu36I Cac8I CfoI
Cfr13I DdeI DpnII DraI DraII
DrdI EaeI Eco81I EcoO109I EcoO65I
EcoRII EcoRV EcoT14I FauI FbaI
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV NspI PleI PpuMI PvuII
RsaI SacI Sau3AI Sau96I ScrFI
SexAI SfaNI SfcI SfiI SmaI
SmlI SphI SspI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XcmI XhoII XmaCI
XmaI