##################################
# NO CUT ENZYMES ( 107 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AflIII AgeI AhdI Alw44I AocI
Aor51HI ApaLI AscI AseI AsnI
Asp700I Asp718I AspEI AspHI AspI
AviII AvrII BamHI BbrPI BbvCI
BfrI BglII BlnI BsaAI BsaBI
BsaWI BseAI BsiHKAI BsiWI Bsp1407I
BspEI BspHI BspLU11I BsrDI BsrGI
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstZ17I Bsu36I CpoI DraI
DrdI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoO65I EcoRV EcoT22I
FseI FspI HincII HindII HpaI
KpnI MamI MfeI MluI MroI
MunI NdeI NheI NotI NruI
NsiI NspV PacI PflFI PflMI
PinAI PmaCI PmeI PmlI PshBI
Psp1406I PvuI RcaI RsrII SacI
SalI SbfI ScaI SexAI SfiI
SfuI SnaBI SpeI Sse8387I SspBI
SspI StuI SwaI Tth111I Van91I
XbaI XmnI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
AccI 1929 GTAGAC GT^[AC][GT]AC
AcsI 1522 GAATTC [GA]^AATT[CT]
AfaI 1837 GTAC GT^AC
AlwNI 208 CAGTCGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 784 GGGCCC GGGCC^C
ApoI 1522 GAATTC [GA]^AATT[CT]
BclI 817 TGATCA T^GATCA
BsaI 153 GGTCTC GGTCTC(1/5)
BsgI 1529 CTGCAC GTGCAG(16/14)
BsmBI 16 GAGACG CGTCTC(1/5)
BsmI 1758 GCATTC GAATGC(1/-1)
BspDI 948 ATCGAT AT^CGAT
BspMI 144 GCAGGT ACCTGC(4/8)
BsrBI 1059 GAGCGG CCGCTC(-3/-3)
BssHII 347 GCGCGC G^CGCGC
BstYI 1955 AGATCC [GA]^GATC[CT]
ClaI 948 ATCGAT AT^CGAT
DraIII 1253 CACCCCGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoRI 1522 GAATTC G^AATTC
FbaI 817 TGATCA T^GATCA
HindIII 936 AAGCTT A^AGCTT
MaeII 1940 ACGT A^CGT
MflI 1955 AGATCC [GA]^GATC[CT]
NcoI 415 CCATGG C^CATGG
PaeR7I 331 CTCGAG C^TCGAG
PleI 1906 GACTC GAGTC(4/5)
PshAI 1714 GACAGAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RsaI 1837 GTAC GT^AC
SapI 809 GAAGAGC GCTCTTC(1/4)
SgrAI 180 CACCGGCG C[GA]^CCGG[CT]G
SmaI 98 CCCGGG CCC^GGG
SphI 1681 GCATGC GCATG^C
TfiI 1580 GATTC G^A[AT]TC
Tsp45I 1950 GTGAC ^GT[GC]AC
XhoI 331 CTCGAG C^TCGAG
XhoII 1955 AGATCC [GA]^GATC[CT]
XmaCI 98 CCCGGG C^CCGGG
XmaI 98 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AcyI 223 GGCGCC G[GA]^CG[CT]C
504 GGCGCC
BalI 418 TGGCCA TGG^CCA
1988 TGGCCA
BbeI 223 GGCGCC GGCGC^C
504 GGCGCC
BbsI 645 GTCTTC GAAGAC(2/6)
1799 GTCTTC
BcgI 614 CGAGGGCGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
59 GCAGCAAGGTCG
BciVI 1871 GGATAC GTATCC(6/5)
2031 GTATCC
BfaI 1197 CTAG C^TAG
2093 CTAG
BglI 305 GCCGCCTCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
411 GCCGCCATGGC
BpuAI 645 GTCTTC GAAGAC(2/6)
1799 GTCTTC
BsaHI 223 GGCGCC G[GA]^CG[CT]C
504 GGCGCC
BsiEI 11 CGGTCG CG[GA][CT]^CG
252 CGACCG
BstXI 196 CCACCAGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1979 CCAAAGTCATGG
EcoNI 686 CCTCATCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1304 CCTCCAGGAGG
EcoT14I 415 CCATGG C^C[AT][AT]GG
997 CCAAGG
Hin1I 223 GGCGCC G[GA]^CG[CT]C
504 GGCGCC
HinfI 1580 GATTC G^A[ACGT]TC
1906 GACTC
HphI 1781 TCACC GGTGA(8/7)
1997 TCACC
KasI 223 GGCGCC G^GCGCC
504 GGCGCC
KspI 129 CCGCGG CCGC^GG
889 CCGCGG
MaeI 1197 CTAG C^TAG
2093 CTAG
MluNI 418 TGGCCA TGG^CCA
1988 TGGCCA
MscI 418 TGGCCA TGG^CCA
1988 TGGCCA
MslI 1160 CACAGCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2059 CATACTTGTG
NaeI 451 GCCGGC GCC^GGC
595 GCCGGC
NarI 223 GGCGCC GG^CGCC
504 GGCGCC
NgoMIV 451 GCCGGC G^CCGGC
595 GCCGGC
NspI 706 GCATGT [GA]CATG^[CT]
1681 GCATGC
PstI 638 CTGCAG CTGCA^G
903 CTGCAG
SacII 129 CCGCGG CCGC^GG
889 CCGCGG
SfoI 223 GGCGCC GGC^GCC
504 GGCGCC
SmlI 331 CTCGAG C^T[CT][GA]AG
1113 CTCAAG
StyI 415 CCATGG C^C[AT][AT]GG
997 CCAAGG
#########################################
# 3 OR MORE CUT ENZYMES ( 81 enzymes) #
#########################################
AccII AciI AluI AlwI AvaI
AvaII BanI BanII BbvI BcnI
BlpI BmyI BpmI Bpu1102I BsaJI
BseRI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BsrFI BsrI BssKI
BstF5I BstNI BstUI Cac8I CelII
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI EarI EcoO109I
EcoRII FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI HinP1I
HpaII ItaI Ksp632I MaeIII MboI
MboII MnlI MseI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV PpuMI PvuII Sau3AI
Sau96I ScrFI SfaNI SfcI TaqI
Tru9I TseI Tsp509I TspRI TthHB8I
XcmI