################################## # NO CUT ENZYMES ( 84 enzymes) # ################################## Acc65I AccIII AgeI AhdI Aor51HI ApaI AscI Asp718I AspEI AvaI AviII AvrII BbeI BbrPI BcgI BcnI BglI BlnI BlpI Bpu1102I BseAI BsiEI BsiWI BsmBI BsoBI BspDI BspEI BsrBI BssHII BssSI BstBI BstEII BstPI BstXI CelII CfoI ClaI CpoI DrdI EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoO65I FseI FspI HaeII HhaI HinP1I KasI KpnI MroI NaeI NarI NciI NcoI NgoMIV NotI NruI NspV PaeR7I PinAI PmaCI PmeI PmlI PshAI PvuI RsrII SalI SapI SbfI SexAI SfiI SfoI SfuI SgrAI SmaI Sse8387I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 47 enzymes) # ################################### AatII 732 GACGTC GACGT^C AccI 5350 GTATAC GT^[AC][GT]AC AclI 4291 AACGTT AA^CGTT AcyI 732 GACGTC G[GA]^CG[CT]C Alw44I 4929 GTGCAC G^TGCAC AocI 909 CCTGAGG CC^T[ACGT]AGG ApaLI 4929 GTGCAC G^TGCAC AspI 3839 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC BalI 5371 TGGCCA TGG^CCA BbvCI 49 CCTCAGC CCTCAGC(-5/-2) BglII 431 AGATCT A^GATCT BsaAI 5221 TACGTA [CT]AC^GT[GA] BsaHI 732 GACGTC G[GA]^CG[CT]C BsaI 516 GGTCTC GGTCTC(1/5) BsaWI 752 ACCGGA [AT]^CCGG[AT] BsgI 4226 GTGCAG GTGCAG(16/14) BspMI 2750 GCAGGT ACCTGC(4/8) Bst1107I 5350 GTATAC GTA^TAC BstAPI 1425 GCAAGGGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstZ17I 5350 GTATAC GTA^TAC Bsu36I 909 CCTGAGG CC^T[ACGT]AGG DraIII 76 CACATAGTG CAC[ACGT][ACGT][ACGT]^GTG EaeI 5371 TGGCCA [CT]^GGCC[GA] EarI 969 GAAGAG CTCTTC(1/4) Eco81I 909 CCTGAGG CC^T[ACGT]AGG EcoRV 1282 GATATC GAT^ATC HgaI 4743 GACGC GACGC(5/10) Hin1I 732 GACGTC G[GA]^CG[CT]C Ksp632I 969 GAAGAG CTCTTC(1/4) MfeI 2584 CAATTG C^AATTG MluI 6 ACGCGT A^CGCGT MluNI 5371 TGGCCA TGG^CCA MscI 5371 TGGCCA TGG^CCA MunI 2584 CAATTG C^AATTG NheI 6107 GCTAGC G^CTAGC PacI 3034 TTAATTAA TTAAT^TAA PflFI 3839 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC PpuMI 1499 AGGTCCT [GA]G^G[AT]CC[CT] Psp1406I 4291 AACGTT AA^CGTT PvuII 811 CAGCTG CAG^CTG SacI 3989 GAGCTC GAGCT^C ScaI 5966 AGTACT AGT^ACT SnaBI 5221 TACGTA TAC^GTA SpeI 5824 ACTAGT A^CTAGT StuI 2397 AGGCCT AGG^CCT Tth111I 3839 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC XcmI 1340 CCATAACTATCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 18 enzymes) # ################################### AlwNI 3404 CAGTTACTG CAG[ACGT][ACGT][ACGT]^CTG 4991 CAGTTACTG BamHI 503 GGATCC G^GATCC 1953 GGATCC BanI 1539 GGTGCC G^G[CT][GA]CC 3005 GGCACC BanII 659 GGGCTC G[GA]GC[CT]^C 3989 GAGCTC BciVI 2975 GGATAC GTATCC(6/5) 2039 GTATCC BsaBI 900 GATCCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 3013 GATGAAGATC BsmAI 517 GTCTC GTCTC(1/5) 5227 GTCTC DraII 1499 AGGTCCT [GA]G^G[ACGT]CC[CT] 5937 AGGCCCT DsaI 1469 CCGCGG C^C[GA][CT]GG 1559 CCGCGG EcoO109I 1499 AGGTCCT [GA]G^G[ACGT]CC[CT] 5937 AGGCCCT HpaI 2212 GTTAAC GTT^AAC 4530 GTTAAC KspI 1469 CCGCGG CCGC^GG 1559 CCGCGG MamI 900 GATCCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 3013 GATGAAGATC NdeI 1230 CATATG CA^TATG 3100 CATATG PleI 208 GAGTC GAGTC(4/5) 4657 GAGTC SacII 1469 CCGCGG CCGC^GG 1559 CCGCGG SphI 4858 GCATGC GCATG^C 5506 GCATGC SwaI 1997 ATTTAAAT ATTT^AAAT 5863 ATTTAAAT ######################################### # 3 OR MORE CUT ENZYMES ( 109 enzymes) # ######################################### AccII AciI AcsI AfaI AflII AflIII AluI AlwI ApoI AseI AsnI Asp700I AspHI AvaII BbsI BbvI BclI BfaI BfrI BmyI BpmI BpuAI BsaJI BseRI BsiHKAI BsiYI BslI BsmFI BsmI Bsp1286I Bsp1407I BspHI BspLU11I BsrDI BsrFI BsrGI BsrI BssKI BstF5I BstNI BstUI BstYI Cac8I Cfr10I Cfr13I DdeI DpnII DraI EcoRI EcoRII EcoT14I EcoT22I FauI FbaI Fnu4HI FokI HaeIII HapII HincII HindII HindIII HinfI HpaII HphI ItaI MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NdeII NlaIII NlaIV NsiI NspI PflMI PshBI PstI RcaI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SspBI SspI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XhoII XmnI