##################################
# NO CUT ENZYMES ( 84 enzymes) #
##################################
Acc65I AccIII AgeI AhdI Aor51HI
ApaI AscI Asp718I AspEI AvaI
AviII AvrII BbeI BbrPI BcgI
BcnI BglI BlnI BlpI Bpu1102I
BseAI BsiEI BsiWI BsmBI BsoBI
BspDI BspEI BsrBI BssHII BssSI
BstBI BstEII BstPI BstXI CelII
CfoI ClaI CpoI DrdI EagI
Eam1105I EclXI Eco47III Eco52I EcoNI
EcoO65I FseI FspI HaeII HhaI
HinP1I KasI KpnI MroI NaeI
NarI NciI NcoI NgoMIV NotI
NruI NspV PaeR7I PinAI PmaCI
PmeI PmlI PshAI PvuI RsrII
SalI SapI SbfI SexAI SfiI
SfoI SfuI SgrAI SmaI Sse8387I
XbaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
AatII 732 GACGTC GACGT^C
AccI 5350 GTATAC GT^[AC][GT]AC
AclI 4291 AACGTT AA^CGTT
AcyI 732 GACGTC G[GA]^CG[CT]C
Alw44I 4929 GTGCAC G^TGCAC
AocI 909 CCTGAGG CC^T[ACGT]AGG
ApaLI 4929 GTGCAC G^TGCAC
AspI 3839 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 5371 TGGCCA TGG^CCA
BbvCI 49 CCTCAGC CCTCAGC(-5/-2)
BglII 431 AGATCT A^GATCT
BsaAI 5221 TACGTA [CT]AC^GT[GA]
BsaHI 732 GACGTC G[GA]^CG[CT]C
BsaI 516 GGTCTC GGTCTC(1/5)
BsaWI 752 ACCGGA [AT]^CCGG[AT]
BsgI 4226 GTGCAG GTGCAG(16/14)
BspMI 2750 GCAGGT ACCTGC(4/8)
Bst1107I 5350 GTATAC GTA^TAC
BstAPI 1425 GCAAGGGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstZ17I 5350 GTATAC GTA^TAC
Bsu36I 909 CCTGAGG CC^T[ACGT]AGG
DraIII 76 CACATAGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 5371 TGGCCA [CT]^GGCC[GA]
EarI 969 GAAGAG CTCTTC(1/4)
Eco81I 909 CCTGAGG CC^T[ACGT]AGG
EcoRV 1282 GATATC GAT^ATC
HgaI 4743 GACGC GACGC(5/10)
Hin1I 732 GACGTC G[GA]^CG[CT]C
Ksp632I 969 GAAGAG CTCTTC(1/4)
MfeI 2584 CAATTG C^AATTG
MluI 6 ACGCGT A^CGCGT
MluNI 5371 TGGCCA TGG^CCA
MscI 5371 TGGCCA TGG^CCA
MunI 2584 CAATTG C^AATTG
NheI 6107 GCTAGC G^CTAGC
PacI 3034 TTAATTAA TTAAT^TAA
PflFI 3839 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 1499 AGGTCCT [GA]G^G[AT]CC[CT]
Psp1406I 4291 AACGTT AA^CGTT
PvuII 811 CAGCTG CAG^CTG
SacI 3989 GAGCTC GAGCT^C
ScaI 5966 AGTACT AGT^ACT
SnaBI 5221 TACGTA TAC^GTA
SpeI 5824 ACTAGT A^CTAGT
StuI 2397 AGGCCT AGG^CCT
Tth111I 3839 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 1340 CCATAACTATCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AlwNI 3404 CAGTTACTG CAG[ACGT][ACGT][ACGT]^CTG
4991 CAGTTACTG
BamHI 503 GGATCC G^GATCC
1953 GGATCC
BanI 1539 GGTGCC G^G[CT][GA]CC
3005 GGCACC
BanII 659 GGGCTC G[GA]GC[CT]^C
3989 GAGCTC
BciVI 2975 GGATAC GTATCC(6/5)
2039 GTATCC
BsaBI 900 GATCCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3013 GATGAAGATC
BsmAI 517 GTCTC GTCTC(1/5)
5227 GTCTC
DraII 1499 AGGTCCT [GA]G^G[ACGT]CC[CT]
5937 AGGCCCT
DsaI 1469 CCGCGG C^C[GA][CT]GG
1559 CCGCGG
EcoO109I 1499 AGGTCCT [GA]G^G[ACGT]CC[CT]
5937 AGGCCCT
HpaI 2212 GTTAAC GTT^AAC
4530 GTTAAC
KspI 1469 CCGCGG CCGC^GG
1559 CCGCGG
MamI 900 GATCCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3013 GATGAAGATC
NdeI 1230 CATATG CA^TATG
3100 CATATG
PleI 208 GAGTC GAGTC(4/5)
4657 GAGTC
SacII 1469 CCGCGG CCGC^GG
1559 CCGCGG
SphI 4858 GCATGC GCATG^C
5506 GCATGC
SwaI 1997 ATTTAAAT ATTT^AAAT
5863 ATTTAAAT
#########################################
# 3 OR MORE CUT ENZYMES ( 109 enzymes) #
#########################################
AccII AciI AcsI AfaI AflII
AflIII AluI AlwI ApoI AseI
AsnI Asp700I AspHI AvaII BbsI
BbvI BclI BfaI BfrI BmyI
BpmI BpuAI BsaJI BseRI BsiHKAI
BsiYI BslI BsmFI BsmI Bsp1286I
Bsp1407I BspHI BspLU11I BsrDI BsrFI
BsrGI BsrI BssKI BstF5I BstNI
BstUI BstYI Cac8I Cfr10I Cfr13I
DdeI DpnII DraI EcoRI EcoRII
EcoT14I EcoT22I FauI FbaI Fnu4HI
FokI HaeIII HapII HincII HindII
HindIII HinfI HpaII HphI ItaI
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MvnI MwoI NdeII
NlaIII NlaIV NsiI NspI PflMI
PshBI PstI RcaI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
SspBI SspI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XhoII XmnI