##################################
# NO CUT ENZYMES ( 75 enzymes) #
##################################
AatII AclI AcyI AflII AgeI
Aor51HI AscI AseI AsnI Asp700I
AvrII BamHI BbeI BciVI BclI
BfrI BglII BlnI BsaBI BsaHI
BsiWI BsmBI BspDI BssHII Bst1107I
BstBI BstZ17I ClaI CpoI Eco47III
EcoRI FbaI FseI Hin1I HincII
HindII HindIII HpaI KasI KspI
MamI MfeI MluI MunI NarI
NdeI NheI NotI NruI NspV
PacI PaeR7I PinAI PmeI PshAI
PshBI Psp1406I PvuI RsrII SacI
SacII SalI SapI SbfI ScaI
SfoI SfuI SgrAI SnaBI SpeI
Sse8387I SwaI XbaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
Acc65I 2546 GGTACC G^GTACC
AhdI 3313 GACAGCTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 617 GTGCAC G^TGCAC
ApaLI 617 GTGCAC G^TGCAC
Asp718I 2546 GGTACC G^GTACC
AspEI 3313 GACAGCTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 2316 GACCCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbrPI 622 CACGTG CAC^GTG
BcgI 446 CGAAAGCGGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BsaAI 622 CACGTG [CT]AC^GT[GA]
BsiEI 1496 CGGCCG CG[GA][CT]^CG
Bsp1407I 3581 TGTACA T^GTACA
BspHI 748 TCATGA T^CATGA
BsrDI 4513 CATTGC GCAATG(2/0)
BsrGI 3581 TGTACA T^GTACA
DraI 3916 TTTAAA TTT^AAA
DrdI 333 GACCGCATCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 1496 CGGCCG C^GGCCG
Eam1105I 3313 GACAGCTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 1496 CGGCCG C^GGCCG
Eco52I 1496 CGGCCG C^GGCCG
EcoNI 3867 CCTTTCTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 2251 GATATC GAT^ATC
EcoT22I 4152 ATGCAT ATGCA^T
KpnI 2546 GGTACC GGTAC^C
NaeI 3770 GCCGGC GCC^GGC
NgoMIV 3770 GCCGGC G^CCGGC
NsiI 4152 ATGCAT ATGCA^T
PflFI 2316 GACCCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 622 CACGTG CAC^GTG
PmlI 622 CACGTG CAC^GTG
RcaI 748 TCATGA T^CATGA
SexAI 4164 ACCTGGT A^CC[AT]GGT
SfiI 271 GGCCAGGGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 1606 CCCGGG CCC^GGG
SphI 3810 GCATGC GCATG^C
SspBI 3581 TGTACA T^GTACA
SspI 2645 AATATT AAT^ATT
Tth111I 2316 GACCCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 1606 CCCGGG C^CCGGG
XmaI 1606 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AccI 1037 GTCTAC GT^[AC][GT]AC
4385 GTCTAC
AccIII 3751 TCCGGA T^CCGGA
3833 TCCGGA
AcsI 212 AAATTT [GA]^AATT[CT]
2200 GAATTT
AlwNI 585 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG
3043 CAGTGTCTG
ApaI 992 GGGCCC GGGCC^C
1501 GGGCCC
ApoI 212 AAATTT [GA]^AATT[CT]
2200 GAATTT
AviII 982 TGCGCA TGC^GCA
1484 TGCGCA
BbvCI 4221 CCTCAGC CCTCAGC(-5/-2)
4452 GCTGAGG
BseAI 3751 TCCGGA T^CCGGA
3833 TCCGGA
BsmI 1921 GAATGC GAATGC(1/-1)
4517 GCATTC
BspEI 3751 TCCGGA T^CCGGA
3833 TCCGGA
BspLU11I 1887 ACATGT A^CATGT
4003 ACATGT
BssSI 1098 CACGAG CACGAG(-5/-1)
2008 CACGAG
BstYI 2676 GGATCT [GA]^GATC[CT]
3289 GGATCT
FspI 982 TGCGCA TGC^GCA
1484 TGCGCA
HgaI 562 GCGTC GACGC(5/10)
678 GCGTC
MflI 2676 GGATCT [GA]^GATC[CT]
3289 GGATCT
MroI 3751 TCCGGA T^CCGGA
3833 TCCGGA
NcoI 156 CCATGG C^CATGG
697 CCATGG
PflMI 3137 CCAAAGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3419 CCAGCATTTGG
Van91I 3137 CCAAAGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3419 CCAGCATTTGG
XcmI 364 CCACCTACTTCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2834 CCACCTGTGAAATGG
XhoII 2676 GGATCT [GA]^GATC[CT]
3289 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 119 enzymes) #
#########################################
AccII AciI AfaI AflIII AluI
AlwI AocI AspHI AvaI AvaII
BalI BanI BanII BbsI BbvI
BcnI BfaI BglI BlpI BmyI
BpmI Bpu1102I BpuAI BsaI BsaJI
BsaWI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrBI BsrFI BsrI BssKI
BstAPI BstEII BstF5I BstNI BstPI
BstUI BstXI Bsu36I Cac8I CelII
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DraIII DsaI EaeI EarI
Eco81I EcoO109I EcoO65I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV NspI PleI PpuMI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I