##################################
# NO CUT ENZYMES ( 86 enzymes) #
##################################
AatII Acc65I AclI AhdI Alw44I
AocI ApaLI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AviII
BamHI BcgI BclI BglII BsiWI
BsmBI Bsp1407I BspLU11I BsrGI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstZ17I Bsu36I CpoI DraI DraIII
DrdI EagI Eam1105I EclXI Eco52I
Eco81I EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI FspI HindIII KpnI
KspI MluI NaeI NdeI NgoMIV
NotI NruI NsiI NspV PacI
PflFI PmeI PshBI Psp1406I PvuI
RsrII SacII SalI SbfI ScaI
SexAI SfiI SfuI SgrAI SmaI
SpeI SphI Sse8387I SspBI SspI
SwaI Tth111I XbaI XmaCI XmaI
XmnI
###################################
# ONE CUT ENZYMES ( 37 enzymes) #
###################################
AccIII 2653 TCCGGA T^CCGGA
AflIII 4171 ACACGT A^C[GA][CT]GT
AgeI 307 ACCGGT A^CCGGT
ApaI 584 GGGCCC GGGCC^C
BalI 4242 TGGCCA TGG^CCA
BbeI 1018 GGCGCC GGCGC^C
BbsI 1861 GTCTTC GAAGAC(2/6)
BglI 2173 GCCAATCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 4054 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 4054 GCTAAGC GC^T[ACGT]AGC
BpuAI 1861 GTCTTC GAAGAC(2/6)
BseAI 2653 TCCGGA T^CCGGA
BsiEI 732 CGACCG CG[GA][CT]^CG
BsmI 1233 GAATGC GAATGC(1/-1)
BspDI 2943 ATCGAT AT^CGAT
BspEI 2653 TCCGGA T^CCGGA
BsrFI 307 ACCGGT [GA]^CCGG[CT]
CelII 4054 GCTAAGC GC^T[ACGT]AGC
Cfr10I 307 ACCGGT [GA]^CCGG[CT]
ClaI 2943 ATCGAT AT^CGAT
EcoNI 4151 CCTATCCTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
KasI 1018 GGCGCC G^GCGCC
MfeI 4730 CAATTG C^AATTG
MluNI 4242 TGGCCA TGG^CCA
MroI 2653 TCCGGA T^CCGGA
MscI 4242 TGGCCA TGG^CCA
MunI 4730 CAATTG C^AATTG
NarI 1018 GGCGCC GG^CGCC
NheI 27 GCTAGC G^CTAGC
PaeR7I 1103 CTCGAG C^TCGAG
PinAI 307 ACCGGT A^CCGGT
PshAI 795 GACAGCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 1295 GAGCTC GAGCT^C
SfoI 1018 GGCGCC GGC^GCC
SnaBI 4428 TACGTA TAC^GTA
StuI 1694 AGGCCT AGG^CCT
XhoI 1103 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AccI 715 GTCTAC GT^[AC][GT]AC
4011 GTAGAC
AccII 321 CGCG CG^CG
4920 CGCG
AflII 3644 CTTAAG C^TTAAG
4344 CTTAAG
Aor51HI 18 AGCGCT AGC^GCT
2674 AGCGCT
BanI 331 GGTGCC G^G[CT][GA]CC
1018 GGCGCC
BbrPI 1378 CACGTG CAC^GTG
1624 CACGTG
BbvCI 2685 CCTCAGC CCTCAGC(-5/-2)
1180 GCTGAGG
BciVI 765 GGATAC GTATCC(6/5)
829 GGATAC
BfrI 3644 CTTAAG C^TTAAG
4344 CTTAAG
BsaBI 1281 GATCGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1818 GATCTCCATC
BspHI 1826 TCATGA T^CATGA
4942 TCATGA
BsrBI 868 GAGCGG CCGCTC(-3/-3)
5192 GAGCGG
BstAPI 1302 GCAGCAGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1609 GCAGAGATTGC
BstUI 321 CGCG CG^CG
4920 CGCG
Eco47III 18 AGCGCT AGC^GCT
2674 AGCGCT
FauI 2858 CCCGC CCCGC(4/6)
2867 GCGGG
HpaI 84 GTTAAC GTT^AAC
5318 GTTAAC
MamI 1281 GATCGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1818 GATCTCCATC
MvnI 321 CGCG CG^CG
4920 CGCG
PflMI 3568 CCACTCCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4663 CCATATTCTGG
PmaCI 1378 CACGTG CAC^GTG
1624 CACGTG
PmlI 1378 CACGTG CAC^GTG
1624 CACGTG
RcaI 1826 TCATGA T^CATGA
4942 TCATGA
SapI 1730 GAAGAGC GCTCTTC(1/4)
3781 GAAGAGC
Van91I 3568 CCACTCCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4663 CCATATTCTGG
XcmI 1258 CCAGCTGTTCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3580 CCAAACCTCTGATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 109 enzymes) #
#########################################
AciI AcsI AcyI AfaI AluI
AlwI AlwNI ApoI AspHI AvaI
AvaII AvrII BanII BbvI BcnI
BfaI BlnI BmyI BpmI BsaAI
BsaHI BsaI BsaJI BsaWI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrDI
BsrI BssKI BstF5I BstNI BstXI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
EcoO109I EcoRII EcoT14I Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HincII HindII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MwoI NciI NcoI NdeII
NlaIII NlaIV NspI PleI PpuMI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII