##################################
# NO CUT ENZYMES ( 53 enzymes) #
##################################
AatII AclI AgeI AhdI Alw44I
Aor51HI ApaLI AspEI AspI AviII
BcgI BsiWI BspDI Bst1107I BstBI
BstZ17I ClaI CpoI DraIII Eam1105I
Eco47III EcoRI EcoRV EcoT22I FseI
FspI HindIII HpaI MfeI MluI
MunI NheI NotI NruI NsiI
NspV PacI PflFI PinAI PmeI
Psp1406I PvuI RsrII SexAI SfiI
SfuI SgrAI SnaBI SpeI SspI
SwaI Tth111I XbaI
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
Acc65I 2408 GGTACC G^GTACC
AccIII 875 TCCGGA T^CCGGA
AscI 448 GGCGCGCC GG^CGCGCC
AseI 4433 ATTAAT AT^TAAT
AsnI 4433 ATTAAT AT^TAAT
Asp718I 2408 GGTACC G^GTACC
AvrII 3006 CCTAGG C^CTAGG
BamHI 4546 GGATCC G^GATCC
BbrPI 585 CACGTG CAC^GTG
BclI 4022 TGATCA T^GATCA
BglI 1606 GCCTTTGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 3006 CCTAGG C^CTAGG
BseAI 875 TCCGGA T^CCGGA
Bsp1407I 1067 TGTACA T^GTACA
BspEI 875 TCCGGA T^CCGGA
BsrGI 1067 TGTACA T^GTACA
BstEII 4057 GGTGACC G^GT[ACGT]ACC
BstPI 4057 GGTGACC G^GT[ACGT]ACC
DraI 4715 TTTAAA TTT^AAA
DrdI 2278 GACAACCTCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 4057 GGTGACC G^GT[ACGT]ACC
FbaI 4022 TGATCA T^GATCA
KpnI 2408 GGTACC GGTAC^C
KspI 3651 CCGCGG CCGC^GG
MroI 875 TCCGGA T^CCGGA
NdeI 2507 CATATG CA^TATG
PaeR7I 490 CTCGAG C^TCGAG
PmaCI 585 CACGTG CAC^GTG
PmlI 585 CACGTG CAC^GTG
PshBI 4433 ATTAAT AT^TAAT
SacI 3259 GAGCTC GAGCT^C
SacII 3651 CCGCGG CCGC^GG
SalI 70 GTCGAC G^TCGAC
SapI 3084 GCTCTTC GCTCTTC(1/4)
SbfI 278 CCTGCAGG CCTGCA^GG
SphI 1602 GCATGC GCATG^C
Sse8387I 278 CCTGCAGG CCTGCA^GG
SspBI 1067 TGTACA T^GTACA
XcmI 4116 CCAGCTGTTTCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 490 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AccI 70 GTCGAC GT^[AC][GT]AC
3277 GTCTAC
AflII 1768 CTTAAG C^TTAAG
5447 CTTAAG
Asp700I 549 GAAGACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2049 GAAGTGGTTC
BalI 3823 TGGCCA TGG^CCA
5421 TGGCCA
BfrI 1768 CTTAAG C^TTAAG
5447 CTTAAG
BsaWI 875 TCCGGA [AT]^CCGG[AT]
2131 ACCGGA
BsmBI 4925 CGTCTC CGTCTC(1/5)
571 GAGACG
BspHI 506 TCATGA T^CATGA
3566 TCATGA
BstAPI 4302 GCAGCAGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
4405 GCAGTGAATGC
EagI 703 CGGCCG C^GGCCG
1132 CGGCCG
EclXI 703 CGGCCG C^GGCCG
1132 CGGCCG
Eco52I 703 CGGCCG C^GGCCG
1132 CGGCCG
MluNI 3823 TGGCCA TGG^CCA
5421 TGGCCA
MscI 3823 TGGCCA TGG^CCA
5421 TGGCCA
NaeI 3039 GCCGGC GCC^GGC
3284 GCCGGC
NgoMIV 3039 GCCGGC G^CCGGC
3284 GCCGGC
RcaI 506 TCATGA T^CATGA
3566 TCATGA
ScaI 1111 AGTACT AGT^ACT
2117 AGTACT
SmaI 359 CCCGGG CCC^GGG
4136 CCCGGG
StuI 3620 AGGCCT AGG^CCT
3948 AGGCCT
XmaCI 359 CCCGGG C^CCGGG
4136 CCCGGG
XmaI 359 CCCGGG C^CCGGG
4136 CCCGGG
XmnI 549 GAAGACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2049 GAAGTGGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 142 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflIII AluI AlwI AlwNI AocI
ApaI ApoI AspHI AvaI AvaII
BanI BanII BbeI BbsI BbvCI
BbvI BciVI BcnI BfaI BglII
BlpI BmyI BpmI Bpu1102I BpuAI
BsaAI BsaBI BsaHI BsaI BsaJI
BseRI BsgI BsiEI BsiHKAI BsiYI
BslI BsmAI BsmFI BsmI BsoBI
Bsp1286I BspLU11I BspMI BsrBI BsrDI
BsrFI BsrI BssHII BssKI BssSI
BstF5I BstNI BstUI BstXI BstYI
Bsu36I Cac8I CelII CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI Eco81I EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HincII HindII HinfI
HpaII HphI ItaI KasI Ksp632I
MaeI MaeII MaeIII MamI MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NarI NciI NcoI NdeII NlaIII
NlaIV NspI PflMI PleI PpuMI
PshAI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SfoI
SmlI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XhoII