##################################
# NO CUT ENZYMES ( 76 enzymes) #
##################################
AatII Acc65I AccI AccIII AcyI
AgeI AhdI AocI Aor51HI AscI
Asp718I AspEI AvrII BbeI BbrPI
BcgI BlnI BsaHI BsaWI BseAI
BsiEI BsiWI BsmBI BspEI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstZ17I Bsu36I CpoI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoO65I
HaeII HgaI Hin1I HincII HindII
HindIII HpaI KasI KpnI KspI
MluI MroI NarI NotI NruI
NspV PacI PaeR7I PinAI PmaCI
PmlI PpuMI PshAI PvuI RsrII
SacII SalI SbfI ScaI SfoI
SfuI SgrAI SnaBI SpeI Sse8387I
XhoI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
AccII 257 CGCG CG^CG
AclI 2861 AACGTT AA^CGTT
AflII 5072 CTTAAG C^TTAAG
Alw44I 1794 GTGCAC G^TGCAC
ApaI 3881 GGGCCC GGGCC^C
ApaLI 1794 GTGCAC G^TGCAC
AseI 3909 ATTAAT AT^TAAT
AsnI 3909 ATTAAT AT^TAAT
AviII 3323 TGCGCA TGC^GCA
BamHI 4474 GGATCC G^GATCC
BfrI 5072 CTTAAG C^TTAAG
BlpI 202 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 202 GCTGAGC GC^T[ACGT]AGC
BsaAI 4166 TACGTG [CT]AC^GT[GA]
BsaBI 5248 GATCCCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BspDI 906 ATCGAT AT^CGAT
BspHI 4049 TCATGA T^CATGA
BsrBI 70 CCGCTC CCGCTC(-3/-3)
BstAPI 5731 GCAGAGCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 257 CGCG CG^CG
CelII 202 GCTGAGC GC^T[ACGT]AGC
ClaI 906 ATCGAT AT^CGAT
DraIII 1721 CACACTGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoRV 400 GATATC GAT^ATC
EcoT22I 2213 ATGCAT ATGCA^T
FseI 242 GGCCGGCC GGCCGG^CC
FspI 3323 TGCGCA TGC^GCA
MamI 5248 GATCCCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MvnI 257 CGCG CG^CG
NheI 4531 GCTAGC G^CTAGC
NsiI 2213 ATGCAT ATGCA^T
PmeI 3446 GTTTAAAC GTTT^AAAC
PshBI 3909 ATTAAT AT^TAAT
Psp1406I 2861 AACGTT AA^CGTT
PstI 4831 CTGCAG CTGCA^G
RcaI 4049 TCATGA T^CATGA
SacI 2244 GAGCTC GAGCT^C
SexAI 3228 ACCAGGT A^CC[AT]GGT
SfiI 237 GGCCAGGCCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 4846 CCCGGG CCC^GGG
SphI 4899 GCATGC GCATG^C
SwaI 3717 ATTTAAAT ATTT^AAAT
XcmI 78 CCATTCCTGAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 4846 CCCGGG C^CCGGG
XmaI 4846 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 37 enzymes) #
###################################
Asp700I 2667 GAAGTATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
5225 GAAAATTTTC
AspHI 1794 GTGCAC G[AT]GC[AT]^C
2244 GAGCTC
AspI 3227 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
5790 GACTCTGTC
AvaI 176 CTCGGG C^[CT]CG[GA]G
4846 CCCGGG
BalI 236 TGGCCA TGG^CCA
5645 TGGCCA
BbsI 593 GAAGAC GAAGAC(2/6)
4011 GTCTTC
BbvCI 4869 CCTCAGC CCTCAGC(-5/-2)
5581 CCTCAGC
BciVI 4261 GGATAC GTATCC(6/5)
423 GTATCC
BclI 3314 TGATCA T^GATCA
3411 TGATCA
BglI 193 GCCAGGCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
238 GCCAGGCCGGC
BpuAI 593 GAAGAC GAAGAC(2/6)
4011 GTCTTC
BsiHKAI 1794 GTGCAC G[AT]GC[AT]^C
2244 GAGCTC
BsoBI 176 CTCGGG C^[CT]CG[GA]G
4846 CCCGGG
BspMI 3182 ACCTGC ACCTGC(4/8)
5554 ACCTGC
BstXI 2349 CCAGTTTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
5016 CCAAAGTGCTGG
CfoI 3324 GCGC GCG^C
4903 GCGC
DrdI 2893 GACACTTTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4964 GACAGGCTGGTC
EaeI 236 TGGCCA [CT]^GGCC[GA]
5645 TGGCCA
EcoNI 452 CCTCAGAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
5388 CCTAAGCTAGG
EcoRI 2586 GAATTC G^AATTC
5067 GAATTC
FbaI 3314 TGATCA T^GATCA
3411 TGATCA
HhaI 3324 GCGC GCG^C
4903 GCGC
HinP1I 3324 GCGC G^CGC
4903 GCGC
MfeI 2518 CAATTG C^AATTG
4361 CAATTG
MluNI 236 TGGCCA TGG^CCA
5645 TGGCCA
MscI 236 TGGCCA TGG^CCA
5645 TGGCCA
MunI 2518 CAATTG C^AATTG
4361 CAATTG
NdeI 4673 CATATG CA^TATG
5680 CATATG
PflFI 3227 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
5790 GACTCTGTC
PflMI 5360 CCATGGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
5637 CCAAAAGATGG
StuI 2444 AGGCCT AGG^CCT
5578 AGGCCT
TaqI 431 TCGA T^CGA
907 TCGA
Tth111I 3227 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
5790 GACTCTGTC
TthHB8I 431 TCGA T^CGA
907 TCGA
Van91I 5360 CCATGGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
5637 CCAAAAGATGG
XbaI 2133 TCTAGA T^CTAGA
4731 TCTAGA
XmnI 2667 GAAGTATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
5225 GAAAATTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 100 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI AlwNI ApoI AvaII BanI
BanII BbvI BcnI BfaI BglII
BmyI BpmI BsaI BsaJI BseRI
BsgI BsiYI BslI BsmAI BsmFI
BsmI Bsp1286I Bsp1407I BspLU11I BsrDI
BsrFI BsrGI BsrI BssKI BstF5I
BstNI BstYI Cac8I Cfr10I Cfr13I
DdeI DpnII DraI DraII DsaI
EarI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MwoI NaeI NciI NcoI
NdeII NgoMIV NlaIII NlaIV NspI
PleI PvuII RsaI SapI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
SspBI SspI StyI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI XhoII