##################################
# NO CUT ENZYMES ( 50 enzymes) #
##################################
AatII AgeI AscI Asp700I BbeI
BglI BsiWI BspDI BsrBI BssHII
BssSI BstBI BstEII BstPI ClaI
CpoI DraI DrdI EcoO65I EcoRI
EcoRV EcoT22I FseI KasI MfeI
MluI MunI NarI NheI NotI
NruI NsiI NspV PacI PinAI
PmeI PvuI RsrII SalI SexAI
SfiI SfoI SfuI SgrAI SnaBI
SpeI SphI SwaI XbaI XmnI
###################################
# ONE CUT ENZYMES ( 53 enzymes) #
###################################
Acc65I 1840 GGTACC G^GTACC
AccIII 3772 TCCGGA T^CCGGA
AclI 1945 AACGTT AA^CGTT
AflII 199 CTTAAG C^TTAAG
Alw44I 5558 GTGCAC G^TGCAC
ApaLI 5558 GTGCAC G^TGCAC
AseI 1011 ATTAAT AT^TAAT
AsnI 1011 ATTAAT AT^TAAT
Asp718I 1840 GGTACC G^GTACC
AviII 4087 TGCGCA TGC^GCA
BamHI 4468 GGATCC G^GATCC
BbrPI 2418 CACGTG CAC^GTG
BcgI 2719 GCAGACCCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 635 GGATAC GTATCC(6/5)
BclI 1905 TGATCA T^GATCA
BfrI 199 CTTAAG C^TTAAG
BsaBI 4688 GATGACTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 3772 TCCGGA [AT]^CCGG[AT]
BseAI 3772 TCCGGA T^CCGGA
BsiEI 4701 CGGCCG CG[GA][CT]^CG
BsmBI 2227 CGTCTC CGTCTC(1/5)
BsmI 5733 GCATTC GAATGC(1/-1)
BspEI 3772 TCCGGA T^CCGGA
BspHI 1557 TCATGA T^CATGA
BsrDI 5014 CATTGC GCAATG(2/0)
Bst1107I 3017 GTATAC GTA^TAC
BstZ17I 3017 GTATAC GTA^TAC
EagI 4701 CGGCCG C^GGCCG
EclXI 4701 CGGCCG C^GGCCG
Eco52I 4701 CGGCCG C^GGCCG
FbaI 1905 TGATCA T^GATCA
FspI 4087 TGCGCA TGC^GCA
HindIII 5456 AAGCTT A^AGCTT
HpaI 1415 GTTAAC GTT^AAC
KpnI 1840 GGTACC GGTAC^C
KspI 4450 CCGCGG CCGC^GG
MamI 4688 GATGACTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 3772 TCCGGA T^CCGGA
PaeR7I 4029 CTCGAG C^TCGAG
PmaCI 2418 CACGTG CAC^GTG
PmlI 2418 CACGTG CAC^GTG
PshBI 1011 ATTAAT AT^TAAT
Psp1406I 1945 AACGTT AA^CGTT
RcaI 1557 TCATGA T^CATGA
SacII 4450 CCGCGG CCGC^GG
SapI 1316 GCTCTTC GCTCTTC(1/4)
SbfI 170 CCTGCAGG CCTGCA^GG
SmaI 4160 CCCGGG CCC^GGG
Sse8387I 170 CCTGCAGG CCTGCA^GG
SspI 1014 AATATT AAT^ATT
XhoI 4029 CTCGAG C^TCGAG
XmaCI 4160 CCCGGG C^CCGGG
XmaI 4160 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AccII 4451 CGCG CG^CG
4542 CGCG
AcyI 1114 GGCGTC G[GA]^CG[CT]C
4080 GACGCC
Aor51HI 4303 AGCGCT AGC^GCT
4339 AGCGCT
BbvCI 2131 GCTGAGG CCTCAGC(-5/-2)
4139 GCTGAGG
BglII 1669 AGATCT A^GATCT
1855 AGATCT
BsaAI 483 TACGTG [CT]AC^GT[GA]
2418 CACGTG
BsaHI 1114 GGCGTC G[GA]^CG[CT]C
4080 GACGCC
Bsp1407I 2065 TGTACA T^GTACA
2778 TGTACA
BspLU11I 780 ACATGT A^CATGT
2460 ACATGT
BsrFI 4699 GCCGGC [GA]^CCGG[CT]
5572 GCCGGC
BsrGI 2065 TGTACA T^GTACA
2778 TGTACA
BstUI 4451 CGCG CG^CG
4542 CGCG
Cfr10I 4699 GCCGGC [GA]^CCGG[CT]
5572 GCCGGC
Eco47III 4303 AGCGCT AGC^GCT
4339 AGCGCT
Hin1I 1114 GGCGTC G[GA]^CG[CT]C
4080 GACGCC
HincII 858 GTTGAC GT[CT]^[GA]AC
1415 GTTAAC
HindII 858 GTTGAC GT[CT]^[GA]AC
1415 GTTAAC
MvnI 4451 CGCG CG^CG
4542 CGCG
NaeI 4699 GCCGGC GCC^GGC
5572 GCCGGC
NdeI 3136 CATATG CA^TATG
3515 CATATG
NgoMIV 4699 GCCGGC G^CCGGC
5572 GCCGGC
PshAI 3706 GACTCATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
3821 GACCTGCGTC
ScaI 195 AGTACT AGT^ACT
593 AGTACT
SspBI 2065 TGTACA T^GTACA
2778 TGTACA
#########################################
# 3 OR MORE CUT ENZYMES ( 131 enzymes) #
#########################################
AccI AciI AcsI AfaI AflIII
AhdI AluI AlwI AlwNI AocI
ApaI ApoI AspEI AspHI AspI
AvaI AvaII AvrII BalI BanI
BanII BbsI BbvI BcnI BfaI
BlnI BlpI BmyI BpmI Bpu1102I
BpuAI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrI BssKI
BstAPI BstF5I BstNI BstXI BstYI
Bsu36I Cac8I CelII CfoI Cfr13I
DdeI DpnII DraII DraIII DsaI
EaeI Eam1105I EarI Eco81I EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MwoI NciI NcoI NdeII
NlaIII NlaIV NspI PflFI PflMI
PleI PpuMI PstI PvuII RsaI
SacI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI Tth111I TthHB8I Van91I XcmI
XhoII