##################################
# NO CUT ENZYMES ( 89 enzymes) #
##################################
AatII AccII AccIII AclI AcyI
AhdI AscI Asp700I AspEI AspI
AviII AvrII BamHI BbeI BbrPI
BcgI BglI BlnI BsaAI BsaHI
BsaI BseAI BsiEI BsiWI BsmBI
BspDI BspEI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstUI BstXI
BstZ17I ClaI CpoI DraIII DsaI
EagI Eam1105I EclXI Eco52I EcoNI
EcoO65I FseI FspI HgaI Hin1I
KasI KspI MaeII MluI MroI
MvnI NaeI NarI NcoI NgoMIV
NheI NotI NruI NspV PacI
PaeR7I PflFI PmaCI PmeI PmlI
PshAI Psp1406I PvuI RsrII SacII
SbfI SexAI SfiI SfoI SfuI
SgrAI SnaBI SpeI SphI Sse8387I
StuI Tth111I XhoI XmnI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
AccI 1921 GTCGAC GT^[AC][GT]AC
AflII 1826 CTTAAG C^TTAAG
AflIII 4154 ACATGT A^C[GA][CT]GT
AgeI 1100 ACCGGT A^CCGGT
Aor51HI 778 AGCGCT AGC^GCT
AvaI 4649 CCCGGG C^[CT]CG[GA]G
BalI 4118 TGGCCA TGG^CCA
BbsI 5048 GAAGAC GAAGAC(2/6)
BfrI 1826 CTTAAG C^TTAAG
BpuAI 5048 GAAGAC GAAGAC(2/6)
BsaWI 1100 ACCGGT [AT]^CCGG[AT]
BsgI 2380 GTGCAG GTGCAG(16/14)
BsmI 2674 GAATGC GAATGC(1/-1)
BsoBI 4649 CCCGGG C^[CT]CG[GA]G
BspLU11I 4154 ACATGT A^CATGT
BspMI 1756 ACCTGC ACCTGC(4/8)
BsrBI 1604 CCGCTC CCGCTC(-3/-3)
BsrDI 3016 CATTGC GCAATG(2/0)
BsrFI 1100 ACCGGT [GA]^CCGG[CT]
BstAPI 855 GCACAAGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Cfr10I 1100 ACCGGT [GA]^CCGG[CT]
DrdI 991 GACGAGCCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 778 AGCGCT AGC^GCT
EcoRV 1402 GATATC GAT^ATC
FauI 1448 GCGGG CCCGC(4/6)
HaeII 778 AGCGCT [GA]GCGC^[CT]
HpaI 3248 GTTAAC GTT^AAC
MfeI 955 CAATTG C^AATTG
MluNI 4118 TGGCCA TGG^CCA
MscI 4118 TGGCCA TGG^CCA
MunI 955 CAATTG C^AATTG
PinAI 1100 ACCGGT A^CCGGT
PpuMI 5836 AGGTCCC [GA]G^G[AT]CC[CT]
SalI 1921 GTCGAC G^TCGAC
ScaI 3953 AGTACT AGT^ACT
SmaI 4649 CCCGGG CCC^GGG
SwaI 1817 ATTTAAAT ATTT^AAAT
XbaI 2843 TCTAGA T^CTAGA
XcmI 1873 CCAAGCACTACTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 4649 CCCGGG C^CCGGG
XmaI 4649 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
Acc65I 3025 GGTACC G^GTACC
4548 GGTACC
Alw44I 4663 GTGCAC G^TGCAC
5893 GTGCAC
AlwNI 1228 CAGATTCTG CAG[ACGT][ACGT][ACGT]^CTG
2410 CAGAAACTG
ApaI 264 GGGCCC GGGCC^C
2565 GGGCCC
ApaLI 4663 GTGCAC G^TGCAC
5893 GTGCAC
Asp718I 3025 GGTACC G^GTACC
4548 GGTACC
AvaII 1601 GGACC G^G[AT]CC
5837 GGTCC
BanI 3025 GGTACC G^G[CT][GA]CC
4548 GGTACC
BbvCI 1980 CCTCAGC CCTCAGC(-5/-2)
4365 GCTGAGG
BciVI 2936 GGATAC GTATCC(6/5)
1775 GTATCC
BclI 2456 TGATCA T^GATCA
3401 TGATCA
BlpI 3135 GCTAAGC GC^T[ACGT]AGC
3903 GCTAAGC
BpmI 2570 CTCCAG CTGGAG(16/14)
5682 CTCCAG
Bpu1102I 3135 GCTAAGC GC^T[ACGT]AGC
3903 GCTAAGC
BstNI 4285 CCAGG CC^[AT]GG
5841 CCAGG
CelII 3135 GCTAAGC GC^T[ACGT]AGC
3903 GCTAAGC
CfoI 779 GCGC GCG^C
4628 GCGC
EaeI 4118 TGGCCA [CT]^GGCC[GA]
5412 CGGCCA
EcoRI 1011 GAATTC G^AATTC
1468 GAATTC
EcoRII 4285 CCAGG ^CC[AT]GG
5841 CCAGG
FbaI 2456 TGATCA T^GATCA
3401 TGATCA
HhaI 779 GCGC GCG^C
4628 GCGC
HinP1I 779 GCGC G^CGC
4628 GCGC
HindIII 113 AAGCTT A^AGCTT
2975 AAGCTT
KpnI 3025 GGTACC GGTAC^C
4548 GGTACC
MspA1I 4252 CAGCTG C[AC]G^C[GT]G
4914 CAGCTG
MvaI 4285 CCAGG CC^[AT]GG
5841 CCAGG
NdeI 1491 CATATG CA^TATG
4377 CATATG
PflMI 3030 CCATCCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4043 CCACTTGATGG
PvuII 4252 CAGCTG CAG^CTG
4914 CAGCTG
SacI 898 GAGCTC GAGCT^C
1125 GAGCTC
SmlI 1826 CTTAAG C^T[CT][GA]AG
2283 CTTGAG
Van91I 3030 CCATCCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4043 CCACTTGATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 95 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AocI ApoI AseI AsnI AspHI
BanII BbvI BcnI BfaI BglII
BmyI BsaBI BsaJI BseRI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
Bsp1407I BspHI BsrGI BsrI BssKI
BstF5I BstYI Bsu36I Cac8I Cfr13I
DdeI DpnII DraI DraII EarI
Eco81I EcoO109I EcoT14I EcoT22I Fnu4HI
FokI HaeIII HapII HincII HindII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeIII MamI MboI MboII
MflI MnlI MseI MslI MspI
MwoI NciI NdeII NlaIII NlaIV
NsiI NspI PleI PshBI PstI
RcaI RsaI SapI Sau3AI Sau96I
ScrFI SfaNI SfcI SspBI SspI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII