##################################
# NO CUT ENZYMES ( 81 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AgeI AhdI Alw44I Aor51HI ApaLI
AscI Asp718I AspEI AspI AviII
BamHI BbrPI BcgI BciVI BfrI
BglI BlpI Bpu1102I BsaAI BsaBI
BseAI BsiWI Bsp1407I BspDI BspEI
BspLU11I BsrGI BssHII BstEII BstPI
CelII ClaI CpoI DraIII DrdI
Eam1105I Eco47III EcoNI EcoO65I EcoRV
FspI HpaI KpnI MamI MluI
MroI NdeI NheI NotI NruI
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI Psp1406I PvuI
RsrII SalI SapI SbfI ScaI
SfiI SgrAI SnaBI SpeI Sse8387I
SspBI SwaI Tth111I Van91I XbaI
XhoI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
AflIII 5694 ACGTGT A^C[GA][CT]GT
ApaI 1104 GGGCCC GGGCC^C
AvrII 6101 CCTAGG C^CTAGG
BbeI 197 GGCGCC GGCGC^C
BlnI 6101 CCTAGG C^CTAGG
BsaI 1388 GGTCTC GGTCTC(1/5)
BsaWI 3353 ACCGGA [AT]^CCGG[AT]
BsiEI 153 CGGCCG CG[GA][CT]^CG
BspHI 2500 TCATGA T^CATGA
BsrBI 3100 GAGCGG CCGCTC(-3/-3)
BsrFI 151 GCCGGC [GA]^CCGG[CT]
BssSI 3118 CTCGTG CACGAG(-5/-1)
Bst1107I 1411 GTATAC GTA^TAC
BstAPI 2748 GCAAGACTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstZ17I 1411 GTATAC GTA^TAC
Cfr10I 151 GCCGGC [GA]^CCGG[CT]
EagI 153 CGGCCG C^GGCCG
EclXI 153 CGGCCG C^GGCCG
Eco52I 153 CGGCCG C^GGCCG
EcoT22I 5116 ATGCAT ATGCA^T
FseI 150 GGCCGGCC GGCCGG^CC
KasI 197 GGCGCC G^GCGCC
KspI 47 CCGCGG CCGC^GG
MfeI 5890 CAATTG C^AATTG
MunI 5890 CAATTG C^AATTG
NaeI 151 GCCGGC GCC^GGC
NarI 197 GGCGCC GG^CGCC
NgoMIV 151 GCCGGC G^CCGGC
NsiI 5116 ATGCAT ATGCA^T
PpuMI 4121 AGGACCC [GA]G^G[AT]CC[CT]
PshAI 3607 GACTTGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 2500 TCATGA T^CATGA
SacI 2687 GAGCTC GAGCT^C
SacII 47 CCGCGG CCGC^GG
SexAI 494 ACCAGGT A^CC[AT]GGT
SfoI 197 GGCGCC GGC^GCC
SphI 1235 GCATGC GCATG^C
StuI 4856 AGGCCT AGG^CCT
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AcyI 39 GGCGTC G[GA]^CG[CT]C
197 GGCGCC
BalI 2025 TGGCCA TGG^CCA
4964 TGGCCA
BanI 197 GGCGCC G^G[CT][GA]CC
1711 GGCACC
BanII 1104 GGGCCC G[GA]GC[CT]^C
2687 GAGCTC
BbvCI 3862 CCTCAGC CCTCAGC(-5/-2)
3996 CCTCAGC
BclI 1097 TGATCA T^GATCA
5953 TGATCA
BpmI 2316 CTCCAG CTGGAG(16/14)
5476 CTGGAG
BsaHI 39 GGCGTC G[GA]^CG[CT]C
197 GGCGCC
BsmBI 105 CGTCTC CGTCTC(1/5)
3932 GAGACG
BstBI 588 TTCGAA TT^CGAA
5387 TTCGAA
BstXI 3872 CCAGGTAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4006 CCAACGTGCTGG
EcoRI 751 GAATTC G^AATTC
5394 GAATTC
FbaI 1097 TGATCA T^GATCA
5953 TGATCA
Hin1I 39 GGCGTC G[GA]^CG[CT]C
197 GGCGCC
HincII 4979 GTTGAC GT[CT]^[GA]AC
5356 GTTGAC
HindII 4979 GTTGAC GT[CT]^[GA]AC
5356 GTTGAC
MluNI 2025 TGGCCA TGG^CCA
4964 TGGCCA
MscI 2025 TGGCCA TGG^CCA
4964 TGGCCA
NspV 588 TTCGAA TT^CGAA
5387 TTCGAA
SfuI 588 TTCGAA TT^CGAA
5387 TTCGAA
SmaI 1129 CCCGGG CCC^GGG
5608 CCCGGG
XcmI 340 CCAACTGGACGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3037 CCATGGTTCTGATGG
XmaCI 1129 CCCGGG C^CCGGG
5608 CCCGGG
XmaI 1129 CCCGGG C^CCGGG
5608 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 115 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AluI AlwI AlwNI AocI ApoI
AseI AsnI Asp700I AspHI AvaI
AvaII BbsI BbvI BcnI BfaI
BglII BmyI BpuAI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI BsoBI Bsp1286I BspMI
BsrDI BsrI BssKI BstF5I BstNI
BstUI BstYI Bsu36I Cac8I CfoI
Cfr13I DdeI DpnII DraI DraII
DsaI EaeI EarI Eco81I EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
HinP1I HindIII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NcoI NdeII NlaIII
NlaIV NspI PleI PshBI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI SspI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII XmnI