##################################
# NO CUT ENZYMES ( 92 enzymes) #
##################################
AatII AccII AccIII AcyI AgeI
AocI Aor51HI AscI AspI AviII
BamHI BbeI BbrPI BbvCI BcnI
BglI BsaAI BsaHI BsaWI BseAI
BsiEI BsiWI BsmBI BspDI BspEI
BspMI BsrBI BssHII BssSI BstAPI
BstBI BstEII BstPI BstUI BstXI
Bsu36I ClaI CpoI DraII DrdI
EagI EclXI Eco47III Eco52I Eco81I
EcoO109I EcoO65I EcoRV FauI FseI
FspI Hin1I KasI KspI MluI
MroI MvnI NaeI NarI NciI
NcoI NgoMIV NotI NruI NspV
PacI PflFI PinAI PmaCI PmeI
PmlI PpuMI PshAI PvuI RsrII
SacII SalI SapI SbfI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SphI Sse8387I Tth111I XbaI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 36 enzymes) #
###################################
Acc65I 678 GGTACC G^GTACC
AhdI 2811 GACATTTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApaI 947 GGGCCC GGGCC^C
Asp718I 678 GGTACC G^GTACC
AspEI 2811 GACATTTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BanI 678 GGTACC G^G[CT][GA]CC
BcgI 2103 GCATTGATGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 1918 AGATCT A^GATCT
BlpI 2487 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 2487 GCTAAGC GC^T[ACGT]AGC
BsaI 392 GAGACC GGTCTC(1/5)
BsgI 509 CTGCAC GTGCAG(16/14)
BsrFI 163 GCCGGT [GA]^CCGG[CT]
Bst1107I 4838 GTATAC GTA^TAC
BstYI 1918 AGATCT [GA]^GATC[CT]
BstZ17I 4838 GTATAC GTA^TAC
CelII 2487 GCTAAGC GC^T[ACGT]AGC
Cfr10I 163 GCCGGT [GA]^CCGG[CT]
DraIII 512 CACTCAGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 328 CCACGG C^C[GA][CT]GG
Eam1105I 2811 GACATTTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 2622 CCTAGTGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HapII 164 CCGG C^CGG
HgaI 87 GACGC GACGC(5/10)
HindIII 1155 AAGCTT A^AGCTT
HpaII 164 CCGG C^CGG
KpnI 678 GGTACC GGTAC^C
MflI 1918 AGATCT [GA]^GATC[CT]
MspI 164 CCGG C^CGG
NheI 2302 GCTAGC G^CTAGC
PvuII 207 CAGCTG CAG^CTG
SacI 13 GAGCTC GAGCT^C
SpeI 3807 ACTAGT A^CTAGT
StuI 2630 AGGCCT AGG^CCT
SwaI 4670 ATTTAAAT ATTT^AAAT
XhoII 1918 AGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 37 enzymes) #
###################################
AflII 3252 CTTAAG C^TTAAG
3430 CTTAAG
AflIII 2516 ACATGT A^C[GA][CT]GT
4696 ACATGT
Alw44I 1067 GTGCAC G^TGCAC
3841 GTGCAC
AlwI 1606 GGATC GGATC(4/5)
1858 GGATC
AlwNI 204 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
1217 CAGTTCCTG
ApaLI 1067 GTGCAC G^TGCAC
3841 GTGCAC
Asp700I 1625 GAAAATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4029 GAAGTCATTC
AvaI 1072 CTCGAG C^[CT]CG[GA]G
1446 CTCGAG
AvrII 3943 CCTAGG C^CTAGG
5626 CCTAGG
BclI 771 TGATCA T^GATCA
3926 TGATCA
BfrI 3252 CTTAAG C^TTAAG
3430 CTTAAG
BlnI 3943 CCTAGG C^CTAGG
5626 CCTAGG
BsaBI 640 GATTGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1601 GATGTGGATC
BseRI 1800 CTCCTC GAGGAG(10/8)
930 GAGGAG
BsmFI 85 GGGAC GGGAC(10/14)
1716 GTCCC
BsoBI 1072 CTCGAG C^[CT]CG[GA]G
1446 CTCGAG
BspHI 1206 TCATGA T^CATGA
2670 TCATGA
BspLU11I 2516 ACATGT A^CATGT
4696 ACATGT
CfoI 2233 GCGC GCG^C
5706 GCGC
EarI 1742 CTCTTC CTCTTC(1/4)
63 GAAGAG
FbaI 771 TGATCA T^GATCA
3926 TGATCA
HaeII 2232 GGCGCT [GA]GCGC^[CT]
5705 AGCGCC
HhaI 2233 GCGC GCG^C
5706 GCGC
HinP1I 2233 GCGC G^CGC
5706 GCGC
HpaI 4385 GTTAAC GTT^AAC
5071 GTTAAC
Ksp632I 1742 CTCTTC CTCTTC(1/4)
63 GAAGAG
MamI 640 GATTGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1601 GATGTGGATC
MspA1I 2 CAGCGG C[AC]G^C[GT]G
207 CAGCTG
PaeR7I 1072 CTCGAG C^TCGAG
1446 CTCGAG
PflMI 838 CCAATCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1915 CCAAGATCTGG
PstI 74 CTGCAG CTGCA^G
360 CTGCAG
RcaI 1206 TCATGA T^CATGA
2670 TCATGA
ScaI 3294 AGTACT AGT^ACT
5611 AGTACT
Van91I 838 CCAATCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1915 CCAAGATCTGG
XcmI 1531 CCATAGCAAGAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3909 CCAGAAGAATATTGG
XhoI 1072 CTCGAG C^TCGAG
1446 CTCGAG
XmnI 1625 GAAAATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4029 GAAGTCATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 93 enzymes) #
#########################################
AccI AciI AclI AcsI AfaI
AluI ApoI AseI AsnI AspHI
AvaII BalI BanII BbsI BbvI
BciVI BfaI BmyI BpmI BpuAI
BsaJI BsiHKAI BsiYI BslI BsmAI
BsmI Bsp1286I Bsp1407I BsrDI BsrGI
BsrI BssKI BstF5I BstNI Cac8I
Cfr13I DdeI DpnII DraI EaeI
EcoRI EcoRII EcoT14I EcoT22I Fnu4HI
FokI HaeIII HincII HindII HinfI
HphI ItaI MaeI MaeII MaeIII
MboI MboII MfeI MluNI MnlI
MscI MseI MslI MunI MvaI
MwoI NdeI NdeII NlaIII NlaIV
NsiI NspI PleI PshBI Psp1406I
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SspBI SspI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I