##################################
# NO CUT ENZYMES ( 63 enzymes) #
##################################
Acc65I AclI AflII ApaI AscI
AseI AsnI Asp718I AspI AviII
AvrII BbrPI BciVI BfrI BlnI
BsiWI BssHII BssSI Bst1107I BstAPI
BstBI BstZ17I CpoI DraI DrdI
EcoRI EcoRV EcoT22I FseI FspI
HindIII HpaI KpnI MfeI MluI
MunI NdeI NheI NotI NruI
NsiI NspV PacI PflFI PmaCI
PmeI PmlI PshBI Psp1406I PvuI
RsrII SalI SbfI SfiI SfuI
SgrAI SnaBI SpeI Sse8387I SspI
SwaI Tth111I XbaI
###################################
# ONE CUT ENZYMES ( 30 enzymes) #
###################################
AgeI 2972 ACCGGT A^CCGGT
AocI 454 CCTGAGG CC^T[ACGT]AGG
BbsI 4152 GAAGAC GAAGAC(2/6)
BbvCI 716 CCTCAGC CCTCAGC(-5/-2)
BclI 1934 TGATCA T^GATCA
BlpI 37 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 37 GCTGAGC GC^T[ACGT]AGC
BpuAI 4152 GAAGAC GAAGAC(2/6)
BsaBI 815 GATTAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BspDI 3247 ATCGAT AT^CGAT
BspLU11I 1970 ACATGT A^CATGT
BsrDI 123 CATTGC GCAATG(2/0)
Bsu36I 454 CCTGAGG CC^T[ACGT]AGG
CelII 37 GCTGAGC GC^T[ACGT]AGC
ClaI 3247 ATCGAT AT^CGAT
EagI 533 CGGCCG C^GGCCG
EclXI 533 CGGCCG C^GGCCG
Eco52I 533 CGGCCG C^GGCCG
Eco81I 454 CCTGAGG CC^T[ACGT]AGG
EcoNI 2553 CCTGGGGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI 1934 TGATCA T^GATCA
KspI 4119 CCGCGG CCGC^GG
MamI 815 GATTAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NcoI 3839 CCATGG C^CATGG
PinAI 2972 ACCGGT A^CCGGT
PshAI 3987 GACTGGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 40 GAGCTC GAGCT^C
SacII 4119 CCGCGG CCGC^GG
ScaI 1421 AGTACT AGT^ACT
SexAI 2639 ACCTGGT A^CC[AT]GGT
###################################
# TWO CUT ENZYMES ( 37 enzymes) #
###################################
AatII 889 GACGTC GACGT^C
3181 GACGTC
AccIII 3158 TCCGGA T^CCGGA
3450 TCCGGA
AcsI 964 AAATTC [GA]^AATT[CT]
1585 GAATTT
AhdI 1090 GACCGCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2612 GACACTGGGTC
Aor51HI 2219 AGCGCT AGC^GCT
2681 AGCGCT
ApoI 964 AAATTC [GA]^AATT[CT]
1585 GAATTT
AspEI 1090 GACCGCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2612 GACACTGGGTC
BbeI 600 GGCGCC GGCGC^C
2956 GGCGCC
BfaI 418 CTAG C^TAG
2512 CTAG
BseAI 3158 TCCGGA T^CCGGA
3450 TCCGGA
BsgI 3289 CTGCAC GTGCAG(16/14)
3132 GTGCAG
BsmBI 3179 GAGACG CGTCTC(1/5)
3370 GAGACG
BsmI 1107 GAATGC GAATGC(1/-1)
2771 GCATTC
Bsp1407I 1973 TGTACA T^GTACA
4103 TGTACA
BspEI 3158 TCCGGA T^CCGGA
3450 TCCGGA
BspHI 329 TCATGA T^CATGA
392 TCATGA
BspMI 3257 ACCTGC ACCTGC(4/8)
3722 ACCTGC
BsrGI 1973 TGTACA T^GTACA
4103 TGTACA
Eam1105I 1090 GACCGCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2612 GACACTGGGTC
Eco47III 2219 AGCGCT AGC^GCT
2681 AGCGCT
HincII 310 GTCAAC GT[CT]^[GA]AC
2473 GTTGAC
HindII 310 GTCAAC GT[CT]^[GA]AC
2473 GTTGAC
KasI 600 GGCGCC G^GCGCC
2956 GGCGCC
MaeI 418 CTAG C^TAG
2512 CTAG
MroI 3158 TCCGGA T^CCGGA
3450 TCCGGA
NaeI 541 GCCGGC GCC^GGC
3793 GCCGGC
NarI 600 GGCGCC GG^CGCC
2956 GGCGCC
NgoMIV 541 GCCGGC G^CCGGC
3793 GCCGGC
PaeR7I 1498 CTCGAG C^TCGAG
3745 CTCGAG
PvuII 737 CAGCTG CAG^CTG
1552 CAGCTG
RcaI 329 TCATGA T^CATGA
392 TCATGA
SapI 1227 GAAGAGC GCTCTTC(1/4)
2904 GCTCTTC
SfoI 600 GGCGCC GGC^GCC
2956 GGCGCC
SphI 2816 GCATGC GCATG^C
2900 GCATGC
SspBI 1973 TGTACA T^GTACA
4103 TGTACA
Tsp509I 965 AATT ^AATT
1586 AATT
XhoI 1498 CTCGAG C^TCGAG
3745 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 128 enzymes) #
#########################################
AccI AccII AciI AcyI AfaI
AflIII AluI Alw44I AlwI AlwNI
ApaLI Asp700I AspHI AvaI AvaII
BalI BamHI BanI BanII BbvI
BcgI BcnI BglI BglII BmyI
BpmI BsaAI BsaHI BsaI BsaJI
BsaWI BseRI BsiEI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BsrBI BsrFI BsrI BssKI BstEII
BstF5I BstNI BstPI BstUI BstXI
BstYI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII DraIII DsaI
EaeI EarI EcoO109I EcoO65I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
NspI PflMI PleI PpuMI PstI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmaI SmlI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
TspRI TthHB8I Van91I XcmI XhoII
XmaCI XmaI XmnI