################################## # NO CUT ENZYMES ( 70 enzymes) # ################################## AatII Acc65I AccI AccII AcyI AgeI Alw44I Aor51HI ApaLI AscI Asp718I AviII AvrII BamHI BbeI BcgI BciVI BglI BlnI BsaHI BsiEI BsiWI BssHII BssSI Bst1107I BstEII BstPI BstUI BstZ17I CpoI DrdI EagI EclXI Eco47III Eco52I EcoNI EcoO65I FseI FspI Hin1I KasI KpnI KspI MluI MvnI NaeI NarI NgoMIV NheI NotI NruI PacI PinAI PmeI PvuI RsrII SacII SalI SbfI ScaI SfiI SfoI SgrAI SmaI SnaBI SpeI Sse8387I SwaI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 43 enzymes) # ################################### AccIII 660 TCCGGA T^CCGGA AclI 3465 AACGTT AA^CGTT AflII 1956 CTTAAG C^TTAAG AhdI 3016 GACTCCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC ApaI 3676 GGGCCC GGGCC^C AspEI 3016 GACTCCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 2257 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 3091 CTCGAG C^[CT]CG[GA]G BbrPI 4672 CACGTG CAC^GTG BbvCI 1480 CCTCAGC CCTCAGC(-5/-2) BclI 5524 TGATCA T^GATCA BfrI 1956 CTTAAG C^TTAAG BlpI 1549 GCTGAGC GC^T[ACGT]AGC Bpu1102I 1549 GCTGAGC GC^T[ACGT]AGC BsaAI 4672 CACGTG [CT]AC^GT[GA] BseAI 660 TCCGGA T^CCGGA BsmBI 1469 CGTCTC CGTCTC(1/5) BsoBI 3091 CTCGAG C^[CT]CG[GA]G BspDI 1516 ATCGAT AT^CGAT BspEI 660 TCCGGA T^CCGGA BspHI 1766 TCATGA T^CATGA BstAPI 3885 GCAGCATCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstBI 3912 TTCGAA TT^CGAA CelII 1549 GCTGAGC GC^T[ACGT]AGC ClaI 1516 ATCGAT AT^CGAT Eam1105I 3016 GACTCCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC FbaI 5524 TGATCA T^GATCA HaeII 5219 AGCGCC [GA]GCGC^[CT] MroI 660 TCCGGA T^CCGGA NspV 3912 TTCGAA TT^CGAA PaeR7I 3091 CTCGAG C^TCGAG PflFI 2257 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC PmaCI 4672 CACGTG CAC^GTG PmlI 4672 CACGTG CAC^GTG PshAI 4391 GACAAATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC Psp1406I 3465 AACGTT AA^CGTT RcaI 1766 TCATGA T^CATGA SacI 2281 GAGCTC GAGCT^C SfuI 3912 TTCGAA TT^CGAA Tth111I 2257 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC XbaI 4544 TCTAGA T^CTAGA XcmI 773 CCAGTGGGGTGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoI 3091 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 28 enzymes) # ################################### AocI 1358 CCTCAGG CC^T[ACGT]AGG 3938 CCTCAGG AseI 2806 ATTAAT AT^TAAT 5227 ATTAAT AsnI 2806 ATTAAT AT^TAAT 5227 ATTAAT BcnI 2973 CCCGG CC^[GC]GG 3876 CCCGG BsaBI 376 GATCTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 2212 GATGCAGATC BsaI 4934 GAGACC GGTCTC(1/5) 1762 GGTCTC BspLU11I 4240 ACATGT A^CATGT 5924 ACATGT BspMI 1182 ACCTGC ACCTGC(4/8) 4554 GCAGGT BsrBI 1803 CCGCTC CCGCTC(-3/-3) 3387 CCGCTC BsrFI 1126 GCCGGT [GA]^CCGG[CT] 1403 ACCGGC BstXI 1655 CCACCCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 5223 CCATATTAATGG Bsu36I 1358 CCTCAGG CC^T[ACGT]AGG 3938 CCTCAGG Cfr10I 1126 GCCGGT [GA]^CCGG[CT] 1403 ACCGGC Eco81I 1358 CCTCAGG CC^T[ACGT]AGG 3938 CCTCAGG EcoRV 3163 GATATC GAT^ATC 4829 GATATC EcoT22I 1202 ATGCAT ATGCA^T 5470 ATGCAT HgaI 1878 GACGC GACGC(5/10) 1858 GCGTC HpaI 2643 GTTAAC GTT^AAC 5559 GTTAAC MamI 376 GATCTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 2212 GATGCAGATC MfeI 2897 CAATTG C^AATTG 4318 CAATTG MunI 2897 CAATTG C^AATTG 4318 CAATTG NciI 2973 CCCGG CC^[GC]GG 3876 CCCGG NsiI 1202 ATGCAT ATGCA^T 5470 ATGCAT PshBI 2806 ATTAAT AT^TAAT 5227 ATTAAT PstI 1979 CTGCAG CTGCA^G 4214 CTGCAG SapI 3329 GCTCTTC GCTCTTC(1/4) 3560 GCTCTTC SexAI 452 ACCTGGT A^CC[AT]GGT 3512 ACCTGGT SphI 412 GCATGC GCATG^C 1385 GCATGC ######################################### # 3 OR MORE CUT ENZYMES ( 117 enzymes) # ######################################### AciI AcsI AfaI AflIII AluI AlwI AlwNI ApoI Asp700I AspHI AvaII BalI BanI BanII BbsI BbvI BfaI BglII BmyI BpmI BpuAI BsaJI BsaWI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI Bsp1286I Bsp1407I BsrDI BsrGI BsrI BssKI BstF5I BstNI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraI DraII DraIII DsaI EaeI EarI EcoO109I EcoRI EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HincII HindII HindIII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MwoI NcoI NdeI NdeII NlaIII NlaIV NspI PflMI PleI PpuMI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SspBI SspI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XhoII XmnI