##################################
# NO CUT ENZYMES ( 70 enzymes) #
##################################
AatII Acc65I AccI AccII AcyI
AgeI Alw44I Aor51HI ApaLI AscI
Asp718I AviII AvrII BamHI BbeI
BcgI BciVI BglI BlnI BsaHI
BsiEI BsiWI BssHII BssSI Bst1107I
BstEII BstPI BstUI BstZ17I CpoI
DrdI EagI EclXI Eco47III Eco52I
EcoNI EcoO65I FseI FspI Hin1I
KasI KpnI KspI MluI MvnI
NaeI NarI NgoMIV NheI NotI
NruI PacI PinAI PmeI PvuI
RsrII SacII SalI SbfI ScaI
SfiI SfoI SgrAI SmaI SnaBI
SpeI Sse8387I SwaI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AccIII 660 TCCGGA T^CCGGA
AclI 3465 AACGTT AA^CGTT
AflII 1956 CTTAAG C^TTAAG
AhdI 3016 GACTCCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApaI 3676 GGGCCC GGGCC^C
AspEI 3016 GACTCCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 2257 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 3091 CTCGAG C^[CT]CG[GA]G
BbrPI 4672 CACGTG CAC^GTG
BbvCI 1480 CCTCAGC CCTCAGC(-5/-2)
BclI 5524 TGATCA T^GATCA
BfrI 1956 CTTAAG C^TTAAG
BlpI 1549 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1549 GCTGAGC GC^T[ACGT]AGC
BsaAI 4672 CACGTG [CT]AC^GT[GA]
BseAI 660 TCCGGA T^CCGGA
BsmBI 1469 CGTCTC CGTCTC(1/5)
BsoBI 3091 CTCGAG C^[CT]CG[GA]G
BspDI 1516 ATCGAT AT^CGAT
BspEI 660 TCCGGA T^CCGGA
BspHI 1766 TCATGA T^CATGA
BstAPI 3885 GCAGCATCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 3912 TTCGAA TT^CGAA
CelII 1549 GCTGAGC GC^T[ACGT]AGC
ClaI 1516 ATCGAT AT^CGAT
Eam1105I 3016 GACTCCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FbaI 5524 TGATCA T^GATCA
HaeII 5219 AGCGCC [GA]GCGC^[CT]
MroI 660 TCCGGA T^CCGGA
NspV 3912 TTCGAA TT^CGAA
PaeR7I 3091 CTCGAG C^TCGAG
PflFI 2257 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 4672 CACGTG CAC^GTG
PmlI 4672 CACGTG CAC^GTG
PshAI 4391 GACAAATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 3465 AACGTT AA^CGTT
RcaI 1766 TCATGA T^CATGA
SacI 2281 GAGCTC GAGCT^C
SfuI 3912 TTCGAA TT^CGAA
Tth111I 2257 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 4544 TCTAGA T^CTAGA
XcmI 773 CCAGTGGGGTGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 3091 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AocI 1358 CCTCAGG CC^T[ACGT]AGG
3938 CCTCAGG
AseI 2806 ATTAAT AT^TAAT
5227 ATTAAT
AsnI 2806 ATTAAT AT^TAAT
5227 ATTAAT
BcnI 2973 CCCGG CC^[GC]GG
3876 CCCGG
BsaBI 376 GATCTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2212 GATGCAGATC
BsaI 4934 GAGACC GGTCTC(1/5)
1762 GGTCTC
BspLU11I 4240 ACATGT A^CATGT
5924 ACATGT
BspMI 1182 ACCTGC ACCTGC(4/8)
4554 GCAGGT
BsrBI 1803 CCGCTC CCGCTC(-3/-3)
3387 CCGCTC
BsrFI 1126 GCCGGT [GA]^CCGG[CT]
1403 ACCGGC
BstXI 1655 CCACCCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
5223 CCATATTAATGG
Bsu36I 1358 CCTCAGG CC^T[ACGT]AGG
3938 CCTCAGG
Cfr10I 1126 GCCGGT [GA]^CCGG[CT]
1403 ACCGGC
Eco81I 1358 CCTCAGG CC^T[ACGT]AGG
3938 CCTCAGG
EcoRV 3163 GATATC GAT^ATC
4829 GATATC
EcoT22I 1202 ATGCAT ATGCA^T
5470 ATGCAT
HgaI 1878 GACGC GACGC(5/10)
1858 GCGTC
HpaI 2643 GTTAAC GTT^AAC
5559 GTTAAC
MamI 376 GATCTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2212 GATGCAGATC
MfeI 2897 CAATTG C^AATTG
4318 CAATTG
MunI 2897 CAATTG C^AATTG
4318 CAATTG
NciI 2973 CCCGG CC^[GC]GG
3876 CCCGG
NsiI 1202 ATGCAT ATGCA^T
5470 ATGCAT
PshBI 2806 ATTAAT AT^TAAT
5227 ATTAAT
PstI 1979 CTGCAG CTGCA^G
4214 CTGCAG
SapI 3329 GCTCTTC GCTCTTC(1/4)
3560 GCTCTTC
SexAI 452 ACCTGGT A^CC[AT]GGT
3512 ACCTGGT
SphI 412 GCATGC GCATG^C
1385 GCATGC
#########################################
# 3 OR MORE CUT ENZYMES ( 117 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI AlwNI ApoI Asp700I AspHI
AvaII BalI BanI BanII BbsI
BbvI BfaI BglII BmyI BpmI
BpuAI BsaJI BsaWI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI Bsp1286I Bsp1407I BsrDI BsrGI
BsrI BssKI BstF5I BstNI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraI DraII DraIII DsaI EaeI
EarI EcoO109I EcoRI EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HhaI HinP1I HincII HindII HindIII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MwoI
NcoI NdeI NdeII NlaIII NlaIV
NspI PflMI PleI PpuMI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SspBI SspI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XhoII XmnI