##################################
# NO CUT ENZYMES (   70 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AcyI        
AgeI        Alw44I      Aor51HI     ApaLI       AscI        
Asp718I     AviII       AvrII       BamHI       BbeI        
BcgI        BciVI       BglI        BlnI        BsaHI       
BsiEI       BsiWI       BssHII      BssSI       Bst1107I    
BstEII      BstPI       BstUI       BstZ17I     CpoI        
DrdI        EagI        EclXI       Eco47III    Eco52I      
EcoNI       EcoO65I     FseI        FspI        Hin1I       
KasI        KpnI        KspI        MluI        MvnI        
NaeI        NarI        NgoMIV      NheI        NotI        
NruI        PacI        PinAI       PmeI        PvuI        
RsrII       SacII       SalI        SbfI        ScaI        
SfiI        SfoI        SgrAI       SmaI        SnaBI       
SpeI        Sse8387I    SwaI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   43 enzymes) #
###################################

AccIII        660  TCCGGA               T^CCGGA
AclI         3465  AACGTT               AA^CGTT
AflII        1956  CTTAAG               C^TTAAG
AhdI         3016  GACTCCAAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApaI         3676  GGGCCC               GGGCC^C
AspEI        3016  GACTCCAAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI         2257  GACTCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvaI         3091  CTCGAG               C^[CT]CG[GA]G
BbrPI        4672  CACGTG               CAC^GTG
BbvCI        1480  CCTCAGC              CCTCAGC(-5/-2)
BclI         5524  TGATCA               T^GATCA
BfrI         1956  CTTAAG               C^TTAAG
BlpI         1549  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I     1549  GCTGAGC              GC^T[ACGT]AGC
BsaAI        4672  CACGTG               [CT]AC^GT[GA]
BseAI         660  TCCGGA               T^CCGGA
BsmBI        1469  CGTCTC               CGTCTC(1/5)
BsoBI        3091  CTCGAG               C^[CT]CG[GA]G
BspDI        1516  ATCGAT               AT^CGAT
BspEI         660  TCCGGA               T^CCGGA
BspHI        1766  TCATGA               T^CATGA
BstAPI       3885  GCAGCATCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI        3912  TTCGAA               TT^CGAA
CelII        1549  GCTGAGC              GC^T[ACGT]AGC
ClaI         1516  ATCGAT               AT^CGAT
Eam1105I     3016  GACTCCAAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FbaI         5524  TGATCA               T^GATCA
HaeII        5219  AGCGCC               [GA]GCGC^[CT]
MroI          660  TCCGGA               T^CCGGA
NspV         3912  TTCGAA               TT^CGAA
PaeR7I       3091  CTCGAG               C^TCGAG
PflFI        2257  GACTCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI        4672  CACGTG               CAC^GTG
PmlI         4672  CACGTG               CAC^GTG
PshAI        4391  GACAAATGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I     3465  AACGTT               AA^CGTT
RcaI         1766  TCATGA               T^CATGA
SacI         2281  GAGCTC               GAGCT^C
SfuI         3912  TTCGAA               TT^CGAA
Tth111I      2257  GACTCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XbaI         4544  TCTAGA               T^CTAGA
XcmI          773  CCAGTGGGGTGATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI         3091  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   28 enzymes) #
###################################

AocI         1358  CCTCAGG              CC^T[ACGT]AGG
             3938  CCTCAGG             
AseI         2806  ATTAAT               AT^TAAT
             5227  ATTAAT              
AsnI         2806  ATTAAT               AT^TAAT
             5227  ATTAAT              
BcnI         2973  CCCGG                CC^[GC]GG
             3876  CCCGG               
BsaBI         376  GATCTGGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             2212  GATGCAGATC          
BsaI         4934  GAGACC               GGTCTC(1/5)
             1762  GGTCTC              
BspLU11I     4240  ACATGT               A^CATGT
             5924  ACATGT              
BspMI        1182  ACCTGC               ACCTGC(4/8)
             4554  GCAGGT              
BsrBI        1803  CCGCTC               CCGCTC(-3/-3)
             3387  CCGCTC              
BsrFI        1126  GCCGGT               [GA]^CCGG[CT]
             1403  ACCGGC              
BstXI        1655  CCACCCTGCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             5223  CCATATTAATGG        
Bsu36I       1358  CCTCAGG              CC^T[ACGT]AGG
             3938  CCTCAGG             
Cfr10I       1126  GCCGGT               [GA]^CCGG[CT]
             1403  ACCGGC              
Eco81I       1358  CCTCAGG              CC^T[ACGT]AGG
             3938  CCTCAGG             
EcoRV        3163  GATATC               GAT^ATC
             4829  GATATC              
EcoT22I      1202  ATGCAT               ATGCA^T
             5470  ATGCAT              
HgaI         1878  GACGC                GACGC(5/10)
             1858  GCGTC               
HpaI         2643  GTTAAC               GTT^AAC
             5559  GTTAAC              
MamI          376  GATCTGGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             2212  GATGCAGATC          
MfeI         2897  CAATTG               C^AATTG
             4318  CAATTG              
MunI         2897  CAATTG               C^AATTG
             4318  CAATTG              
NciI         2973  CCCGG                CC^[GC]GG
             3876  CCCGG               
NsiI         1202  ATGCAT               ATGCA^T
             5470  ATGCAT              
PshBI        2806  ATTAAT               AT^TAAT
             5227  ATTAAT              
PstI         1979  CTGCAG               CTGCA^G
             4214  CTGCAG              
SapI         3329  GCTCTTC              GCTCTTC(1/4)
             3560  GCTCTTC             
SexAI         452  ACCTGGT              A^CC[AT]GGT
             3512  ACCTGGT             
SphI          412  GCATGC               GCATG^C
             1385  GCATGC              


#########################################
# 3 OR MORE CUT ENZYMES (  117 enzymes) #
#########################################

AciI        AcsI        AfaI        AflIII      AluI        
AlwI        AlwNI       ApoI        Asp700I     AspHI       
AvaII       BalI        BanI        BanII       BbsI        
BbvI        BfaI        BglII       BmyI        BpmI        
BpuAI       BsaJI       BsaWI       BseRI       BsgI        
BsiHKAI     BsiYI       BslI        BsmAI       BsmFI       
BsmI        Bsp1286I    Bsp1407I    BsrDI       BsrGI       
BsrI        BssKI       BstF5I      BstNI       BstYI       
Cac8I       CfoI        Cfr13I      DdeI        DpnII       
DraI        DraII       DraIII      DsaI        EaeI        
EarI        EcoO109I    EcoRI       EcoRII      EcoT14I     
FauI        Fnu4HI      FokI        HaeIII      HapII       
HhaI        HinP1I      HincII      HindII      HindIII     
HinfI       HpaII       HphI        ItaI        Ksp632I     
MaeI        MaeII       MaeIII      MboI        MboII       
MflI        MluNI       MnlI        MscI        MseI        
MslI        MspA1I      MspI        MvaI        MwoI        
NcoI        NdeI        NdeII       NlaIII      NlaIV       
NspI        PflMI       PleI        PpuMI       PvuII       
RsaI        Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        SmlI        SspBI       SspI        StuI        
StyI        TaqI        TfiI        Tru9I       TseI        
Tsp45I      Tsp509I     TspRI       TthHB8I     Van91I      
XhoII       XmnI