################################## # NO CUT ENZYMES ( 55 enzymes) # ################################## AclI AcsI AflII AgeI Aor51HI ApoI AscI AseI AsnI AviII BamHI BclI BfrI BsaBI BsiWI BsmI BspDI BspHI Bst1107I BstBI BstZ17I ClaI DraI Eco47III EcoRI EcoRV FbaI FseI FspI KspI MamI MfeI MunI NcoI NheI NotI NruI NspV PacI PinAI PmeI PshBI Psp1406I PvuI RcaI SacII SalI ScaI SexAI SfuI SnaBI SpeI SspI SwaI XbaI ################################### # ONE CUT ENZYMES ( 30 enzymes) # ################################### Acc65I 2421 GGTACC G^GTACC AccI 3959 GTCTAC GT^[AC][GT]AC Alw44I 5557 GTGCAC G^TGCAC ApaLI 5557 GTGCAC G^TGCAC Asp718I 2421 GGTACC G^GTACC BbrPI 4565 CACGTG CAC^GTG BcgI 2722 CGACCGCGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) Bsp1407I 5436 TGTACA T^GTACA BspLU11I 657 ACATGT A^CATGT BsrDI 1314 GCAATG GCAATG(2/0) BsrGI 5436 TGTACA T^GTACA BssSI 1535 CACGAG CACGAG(-5/-1) CpoI 45 CGGTCCG CG^G[AT]CCG DraIII 3152 CACCGTGTG CAC[ACGT][ACGT][ACGT]^GTG HindIII 391 AAGCTT A^AGCTT HpaI 359 GTTAAC GTT^AAC KpnI 2421 GGTACC GGTAC^C MluI 447 ACGCGT A^CGCGT NdeI 5440 CATATG CA^TATG PmaCI 4565 CACGTG CAC^GTG PmlI 4565 CACGTG CAC^GTG PshAI 5501 GACGCCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC RsrII 45 CGGTCCG CG^G[AT]CCG SapI 3365 GAAGAGC GCTCTTC(1/4) SbfI 3568 CCTGCAGG CCTGCA^GG SfiI 3205 GGCCGTCCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SgrAI 4598 CGCCGGCG C[GA]^CCGG[CT]G Sse8387I 3568 CCTGCAGG CCTGCA^GG SspBI 5436 TGTACA T^GTACA TfiI 1169 GATTC G^A[AT]TC ################################### # TWO CUT ENZYMES ( 35 enzymes) # ################################### AatII 3026 GACGTC GACGT^C 5493 GACGTC AocI 431 CCTGAGG CC^T[ACGT]AGG 3835 CCTGAGG Asp700I 3187 GAAGGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 3365 GAAGAGCTTC AspI 3023 GACGACGTC GAC[ACGT]^[ACGT][ACGT]GTC 3727 GACCAAGTC BalI 1695 TGGCCA TGG^CCA 3781 TGGCCA BbeI 81 GGCGCC GGCGC^C 4602 GGCGCC BbvCI 3414 CCTCAGC CCTCAGC(-5/-2) 1279 GCTGAGG BciVI 509 GGATAC GTATCC(6/5) 4226 GGATAC BglI 1673 GCCCTGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 3206 GCCGTCCAGGC BglII 3969 AGATCT A^GATCT 4850 AGATCT BsaAI 776 TACGTG [CT]AC^GT[GA] 4565 CACGTG BssHII 8 GCGCGC G^CGCGC 60 GCGCGC BstAPI 757 GCACCCACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 4029 GCATGCCATGC Bsu36I 431 CCTGAGG CC^T[ACGT]AGG 3835 CCTGAGG EagI 3055 CGGCCG C^GGCCG 4005 CGGCCG EclXI 3055 CGGCCG C^GGCCG 4005 CGGCCG Eco52I 3055 CGGCCG C^GGCCG 4005 CGGCCG Eco81I 431 CCTGAGG CC^T[ACGT]AGG 3835 CCTGAGG EcoT22I 2872 ATGCAT ATGCA^T 4036 ATGCAT KasI 81 GGCGCC G^GCGCC 4602 GGCGCC MluNI 1695 TGGCCA TGG^CCA 3781 TGGCCA MscI 1695 TGGCCA TGG^CCA 3781 TGGCCA NarI 81 GGCGCC GG^CGCC 4602 GGCGCC NsiI 2872 ATGCAT ATGCA^T 4036 ATGCAT PaeR7I 128 CTCGAG C^TCGAG 2917 CTCGAG PflFI 3023 GACGACGTC GAC[ACGT]^[ACGT][ACGT]GTC 3727 GACCAAGTC PvuII 2112 CAGCTG CAG^CTG 5341 CAGCTG SfoI 81 GGCGCC GGC^GCC 4602 GGCGCC SmaI 297 CCCGGG CCC^GGG 1555 CCCGGG SphI 496 GCATGC GCATG^C 4029 GCATGC Tth111I 3023 GACGACGTC GAC[ACGT]^[ACGT][ACGT]GTC 3727 GACCAAGTC XhoI 128 CTCGAG C^TCGAG 2917 CTCGAG XmaCI 297 CCCGGG C^CCGGG 1555 CCCGGG XmaI 297 CCCGGG C^CCGGG 1555 CCCGGG XmnI 3187 GAAGGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 3365 GAAGAGCTTC ######################################### # 3 OR MORE CUT ENZYMES ( 138 enzymes) # ######################################### AccII AccIII AciI AcyI AfaI AflIII AhdI AluI AlwI AlwNI ApaI AspEI AspHI AvaI AvaII AvrII BanI BanII BbsI BbvI BcnI BfaI BlnI BlpI BmyI BpmI Bpu1102I BpuAI BsaHI BsaI BsaJI BsaWI BseAI BseRI BsgI BsiEI BsiHKAI BsiYI BslI BsmAI BsmBI BsmFI BsoBI Bsp1286I BspEI BspMI BsrBI BsrFI BsrI BssKI BstEII BstF5I BstNI BstPI BstUI BstXI BstYI Cac8I CelII CfoI Cfr10I Cfr13I DdeI DpnII DraII DrdI DsaI EaeI Eam1105I EarI EcoNI EcoO109I EcoO65I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MroI MseI MslI MspA1I MspI MvaI MvnI MwoI NaeI NciI NdeII NgoMIV NlaIII NlaIV NspI PflMI PleI PpuMI PstI RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StuI StyI TaqI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI XhoII