##################################
# NO CUT ENZYMES ( 55 enzymes) #
##################################
AclI AcsI AflII AgeI Aor51HI
ApoI AscI AseI AsnI AviII
BamHI BclI BfrI BsaBI BsiWI
BsmI BspDI BspHI Bst1107I BstBI
BstZ17I ClaI DraI Eco47III EcoRI
EcoRV FbaI FseI FspI KspI
MamI MfeI MunI NcoI NheI
NotI NruI NspV PacI PinAI
PmeI PshBI Psp1406I PvuI RcaI
SacII SalI ScaI SexAI SfuI
SnaBI SpeI SspI SwaI XbaI
###################################
# ONE CUT ENZYMES ( 30 enzymes) #
###################################
Acc65I 2421 GGTACC G^GTACC
AccI 3959 GTCTAC GT^[AC][GT]AC
Alw44I 5557 GTGCAC G^TGCAC
ApaLI 5557 GTGCAC G^TGCAC
Asp718I 2421 GGTACC G^GTACC
BbrPI 4565 CACGTG CAC^GTG
BcgI 2722 CGACCGCGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
Bsp1407I 5436 TGTACA T^GTACA
BspLU11I 657 ACATGT A^CATGT
BsrDI 1314 GCAATG GCAATG(2/0)
BsrGI 5436 TGTACA T^GTACA
BssSI 1535 CACGAG CACGAG(-5/-1)
CpoI 45 CGGTCCG CG^G[AT]CCG
DraIII 3152 CACCGTGTG CAC[ACGT][ACGT][ACGT]^GTG
HindIII 391 AAGCTT A^AGCTT
HpaI 359 GTTAAC GTT^AAC
KpnI 2421 GGTACC GGTAC^C
MluI 447 ACGCGT A^CGCGT
NdeI 5440 CATATG CA^TATG
PmaCI 4565 CACGTG CAC^GTG
PmlI 4565 CACGTG CAC^GTG
PshAI 5501 GACGCCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RsrII 45 CGGTCCG CG^G[AT]CCG
SapI 3365 GAAGAGC GCTCTTC(1/4)
SbfI 3568 CCTGCAGG CCTGCA^GG
SfiI 3205 GGCCGTCCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SgrAI 4598 CGCCGGCG C[GA]^CCGG[CT]G
Sse8387I 3568 CCTGCAGG CCTGCA^GG
SspBI 5436 TGTACA T^GTACA
TfiI 1169 GATTC G^A[AT]TC
###################################
# TWO CUT ENZYMES ( 35 enzymes) #
###################################
AatII 3026 GACGTC GACGT^C
5493 GACGTC
AocI 431 CCTGAGG CC^T[ACGT]AGG
3835 CCTGAGG
Asp700I 3187 GAAGGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3365 GAAGAGCTTC
AspI 3023 GACGACGTC GAC[ACGT]^[ACGT][ACGT]GTC
3727 GACCAAGTC
BalI 1695 TGGCCA TGG^CCA
3781 TGGCCA
BbeI 81 GGCGCC GGCGC^C
4602 GGCGCC
BbvCI 3414 CCTCAGC CCTCAGC(-5/-2)
1279 GCTGAGG
BciVI 509 GGATAC GTATCC(6/5)
4226 GGATAC
BglI 1673 GCCCTGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
3206 GCCGTCCAGGC
BglII 3969 AGATCT A^GATCT
4850 AGATCT
BsaAI 776 TACGTG [CT]AC^GT[GA]
4565 CACGTG
BssHII 8 GCGCGC G^CGCGC
60 GCGCGC
BstAPI 757 GCACCCACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
4029 GCATGCCATGC
Bsu36I 431 CCTGAGG CC^T[ACGT]AGG
3835 CCTGAGG
EagI 3055 CGGCCG C^GGCCG
4005 CGGCCG
EclXI 3055 CGGCCG C^GGCCG
4005 CGGCCG
Eco52I 3055 CGGCCG C^GGCCG
4005 CGGCCG
Eco81I 431 CCTGAGG CC^T[ACGT]AGG
3835 CCTGAGG
EcoT22I 2872 ATGCAT ATGCA^T
4036 ATGCAT
KasI 81 GGCGCC G^GCGCC
4602 GGCGCC
MluNI 1695 TGGCCA TGG^CCA
3781 TGGCCA
MscI 1695 TGGCCA TGG^CCA
3781 TGGCCA
NarI 81 GGCGCC GG^CGCC
4602 GGCGCC
NsiI 2872 ATGCAT ATGCA^T
4036 ATGCAT
PaeR7I 128 CTCGAG C^TCGAG
2917 CTCGAG
PflFI 3023 GACGACGTC GAC[ACGT]^[ACGT][ACGT]GTC
3727 GACCAAGTC
PvuII 2112 CAGCTG CAG^CTG
5341 CAGCTG
SfoI 81 GGCGCC GGC^GCC
4602 GGCGCC
SmaI 297 CCCGGG CCC^GGG
1555 CCCGGG
SphI 496 GCATGC GCATG^C
4029 GCATGC
Tth111I 3023 GACGACGTC GAC[ACGT]^[ACGT][ACGT]GTC
3727 GACCAAGTC
XhoI 128 CTCGAG C^TCGAG
2917 CTCGAG
XmaCI 297 CCCGGG C^CCGGG
1555 CCCGGG
XmaI 297 CCCGGG C^CCGGG
1555 CCCGGG
XmnI 3187 GAAGGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3365 GAAGAGCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 138 enzymes) #
#########################################
AccII AccIII AciI AcyI AfaI
AflIII AhdI AluI AlwI AlwNI
ApaI AspEI AspHI AvaI AvaII
AvrII BanI BanII BbsI BbvI
BcnI BfaI BlnI BlpI BmyI
BpmI Bpu1102I BpuAI BsaHI BsaI
BsaJI BsaWI BseAI BseRI BsgI
BsiEI BsiHKAI BsiYI BslI BsmAI
BsmBI BsmFI BsoBI Bsp1286I BspEI
BspMI BsrBI BsrFI BsrI BssKI
BstEII BstF5I BstNI BstPI BstUI
BstXI BstYI Cac8I CelII CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DrdI DsaI EaeI Eam1105I EarI
EcoNI EcoO109I EcoO65I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HincII HindII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MroI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NaeI NciI NdeII
NgoMIV NlaIII NlaIV NspI PflMI
PleI PpuMI PstI RsaI SacI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StuI StyI TaqI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XcmI XhoII