##################################
# NO CUT ENZYMES ( 38 enzymes) #
##################################
AclI AflII AgeI AscI AviII
BamHI BclI BfrI BsiWI BsmI
BspDI BspHI Bst1107I BstBI BstZ17I
ClaI DraI EcoRV FbaI FseI
FspI MfeI MunI NotI NruI
NspV PacI PinAI PmeI Psp1406I
RcaI ScaI SexAI SfuI SnaBI
SpeI SwaI XbaI
###################################
# ONE CUT ENZYMES ( 24 enzymes) #
###################################
Aor51HI 7812 AGCGCT AGC^GCT
AseI 5941 ATTAAT AT^TAAT
AsnI 5941 ATTAAT AT^TAAT
BsaBI 7676 GATTCACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BssSI 1535 CACGAG CACGAG(-5/-1)
CpoI 45 CGGTCCG CG^G[AT]CCG
DraIII 3152 CACCGTGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco47III 7812 AGCGCT AGC^GCT
EcoRI 6602 GAATTC G^AATTC
HindIII 391 AAGCTT A^AGCTT
HpaI 359 GTTAAC GTT^AAC
KspI 5494 CCGCGG CCGC^GG
MamI 7676 GATTCACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluI 447 ACGCGT A^CGCGT
NdeI 8991 CATATG CA^TATG
NheI 6190 GCTAGC G^CTAGC
PshBI 5941 ATTAAT AT^TAAT
PvuI 5271 CGATCG CGAT^CG
RsrII 45 CGGTCCG CG^G[AT]CCG
SacII 5494 CCGCGG CCGC^GG
SalI 5734 GTCGAC G^TCGAC
SapI 3365 GAAGAGC GCTCTTC(1/4)
SfiI 3205 GGCCGTCCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SgrAI 4574 CGCCGGCG C[GA]^CCGG[CT]G
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AatII 3026 GACGTC GACGT^C
9044 GACGTC
Acc65I 2421 GGTACC G^GTACC
5289 GGTACC
AccI 3959 GTCTAC GT^[AC][GT]AC
5734 GTCGAC
Alw44I 5482 GTGCAC G^TGCAC
9108 GTGCAC
ApaLI 5482 GTGCAC G^TGCAC
9108 GTGCAC
Asp718I 2421 GGTACC G^GTACC
5289 GGTACC
BbrPI 4541 CACGTG CAC^GTG
7239 CACGTG
BglII 3969 AGATCT A^GATCT
8460 AGATCT
Bsp1407I 5367 TGTACA T^GTACA
8987 TGTACA
BsrDI 1314 GCAATG GCAATG(2/0)
6812 GCAATG
BsrGI 5367 TGTACA T^GTACA
8987 TGTACA
BssHII 8 GCGCGC G^CGCGC
60 GCGCGC
EagI 3055 CGGCCG C^GGCCG
4005 CGGCCG
EclXI 3055 CGGCCG C^GGCCG
4005 CGGCCG
Eco52I 3055 CGGCCG C^GGCCG
4005 CGGCCG
KpnI 2421 GGTACC GGTAC^C
5289 GGTACC
PaeR7I 128 CTCGAG C^TCGAG
2917 CTCGAG
PmaCI 4541 CACGTG CAC^GTG
7239 CACGTG
PmlI 4541 CACGTG CAC^GTG
7239 CACGTG
PshAI 6945 GACGGGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
9052 GACGCCTGTC
SbfI 3568 CCTGCAGG CCTGCA^GG
4702 CCTGCAGG
SphI 496 GCATGC GCATG^C
4029 GCATGC
Sse8387I 3568 CCTGCAGG CCTGCA^GG
4702 CCTGCAGG
SspBI 5367 TGTACA T^GTACA
8987 TGTACA
SspI 5911 AATATT AAT^ATT
6047 AATATT
XhoI 128 CTCGAG C^TCGAG
2917 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 170 enzymes) #
#########################################
AccII AccIII AciI AcsI AcyI
AfaI AflIII AhdI AluI AlwI
AlwNI AocI ApaI ApoI Asp700I
AspEI AspHI AspI AvaI AvaII
AvrII BalI BanI BanII BbeI
BbsI BbvCI BbvI BcgI BciVI
BcnI BfaI BglI BlnI BlpI
BmyI BpmI Bpu1102I BpuAI BsaAI
BsaHI BsaI BsaJI BsaWI BseAI
BseRI BsgI BsiEI BsiHKAI BsiYI
BslI BsmAI BsmBI BsmFI BsoBI
Bsp1286I BspEI BspLU11I BspMI BsrBI
BsrFI BsrI BssKI BstAPI BstEII
BstF5I BstNI BstPI BstUI BstXI
BstYI Bsu36I Cac8I CelII CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DrdI DsaI EaeI Eam1105I EarI
Eco81I EcoNI EcoO109I EcoO65I EcoRII
EcoT14I EcoT22I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HincII HindII HinfI
HpaII HphI ItaI KasI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MroI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NaeI NarI NciI
NcoI NdeII NgoMIV NlaIII NlaIV
NsiI NspI PflFI PflMI PleI
PpuMI PstI PvuII RsaI SacI
Sau3AI Sau96I ScrFI SfaNI SfcI
SfoI SmaI SmlI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI Tth111I TthHB8I Van91I
XcmI XhoII XmaCI XmaI XmnI