##################################
# NO CUT ENZYMES ( 63 enzymes) #
##################################
AccIII AclI AgeI AhdI Aor51HI
AscI AseI AsnI AspEI AviII
AvrII BamHI BbvCI BclI BglII
BlnI BseAI BsiEI BsiWI BspDI
BspEI BssHII BstBI BstYI ClaI
CpoI DrdI EagI Eam1105I EclXI
Eco47III Eco52I EcoRI EcoRV FbaI
FseI FspI HpaI MflI MluI
MroI NheI NotI NruI NspV
PacI PinAI PmeI PshAI PshBI
Psp1406I PvuI RsrII SalI SbfI
SfuI SgrAI SnaBI SpeI SphI
Sse8387I SwaI XhoII
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
Acc65I 1860 GGTACC G^GTACC
AocI 1566 CCTGAGG CC^T[ACGT]AGG
Asp718I 1860 GGTACC G^GTACC
AspI 1462 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 5112 TGGCCA TGG^CCA
BbeI 4361 GGCGCC GGCGC^C
BsaWI 2054 ACCGGA [AT]^CCGG[AT]
BspLU11I 5326 ACATGT A^CATGT
Bst1107I 4786 GTATAC GTA^TAC
BstEII 1986 GGTAACC G^GT[ACGT]ACC
BstPI 1986 GGTAACC G^GT[ACGT]ACC
BstZ17I 4786 GTATAC GTA^TAC
Bsu36I 1566 CCTGAGG CC^T[ACGT]AGG
DraI 338 TTTAAA TTT^AAA
Eco81I 1566 CCTGAGG CC^T[ACGT]AGG
EcoO65I 1986 GGTAACC G^GT[ACGT]ACC
EcoT22I 4018 ATGCAT ATGCA^T
HincII 5452 GTCAAC GT[CT]^[GA]AC
HindII 5452 GTCAAC GT[CT]^[GA]AC
HindIII 4818 AAGCTT A^AGCTT
KasI 4361 GGCGCC G^GCGCC
KpnI 1860 GGTACC GGTAC^C
KspI 1870 CCGCGG CCGC^GG
MfeI 4403 CAATTG C^AATTG
MluNI 5112 TGGCCA TGG^CCA
MscI 5112 TGGCCA TGG^CCA
MunI 4403 CAATTG C^AATTG
NarI 4361 GGCGCC GG^CGCC
NcoI 4643 CCATGG C^CATGG
NdeI 293 CATATG CA^TATG
NsiI 4018 ATGCAT ATGCA^T
PflFI 1462 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
SacII 1870 CCGCGG CCGC^GG
SfoI 4361 GGCGCC GGC^GCC
SspI 3914 AATATT AAT^ATT
StuI 5487 AGGCCT AGG^CCT
Tth111I 1462 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 494 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AflII 2959 CTTAAG C^TTAAG
3983 CTTAAG
ApaI 5130 GGGCCC GGGCC^C
5243 GGGCCC
BbrPI 70 CACGTG CAC^GTG
5190 CACGTG
BfrI 2959 CTTAAG C^TTAAG
3983 CTTAAG
BlpI 690 GCTGAGC GC^T[ACGT]AGC
939 GCTGAGC
Bpu1102I 690 GCTGAGC GC^T[ACGT]AGC
939 GCTGAGC
BsmBI 5020 CGTCTC CGTCTC(1/5)
3148 GAGACG
BsmI 1165 GCATTC GAATGC(1/-1)
4020 GCATTC
Bsp1407I 962 TGTACA T^GTACA
5443 TGTACA
BspHI 324 TCATGA T^CATGA
903 TCATGA
BsrBI 3078 CCGCTC CCGCTC(-3/-3)
2415 GAGCGG
BsrDI 577 CATTGC GCAATG(2/0)
3573 CATTGC
BsrGI 962 TGTACA T^GTACA
5443 TGTACA
BstXI 2786 CCAAAGTGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3177 CCAGCAGAATGG
CelII 690 GCTGAGC GC^T[ACGT]AGC
939 GCTGAGC
NaeI 1173 GCCGGC GCC^GGC
3368 GCCGGC
NgoMIV 1173 GCCGGC G^CCGGC
3368 GCCGGC
NspI 1802 ACATGC [GA]CATG^[CT]
5326 ACATGT
PaeR7I 2137 CTCGAG C^TCGAG
5144 CTCGAG
PmaCI 70 CACGTG CAC^GTG
5190 CACGTG
PmlI 70 CACGTG CAC^GTG
5190 CACGTG
RcaI 324 TCATGA T^CATGA
903 TCATGA
SacI 653 GAGCTC GAGCT^C
2114 GAGCTC
ScaI 377 AGTACT AGT^ACT
3609 AGTACT
SexAI 436 ACCTGGT A^CC[AT]GGT
890 ACCTGGT
SfiI 2268 GGCCCCGCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
4476 GGCCTCGGTGGCC
SspBI 962 TGTACA T^GTACA
5443 TGTACA
XhoI 2137 CTCGAG C^TCGAG
5144 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 129 enzymes) #
#########################################
AatII AccI AccII AciI AcsI
AcyI AfaI AflIII AluI Alw44I
AlwI AlwNI ApaLI ApoI Asp700I
AspHI AvaI AvaII BanI BanII
BbsI BbvI BcgI BciVI BcnI
BfaI BglI BmyI BpmI BpuAI
BsaAI BsaBI BsaHI BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrFI BsrI BssKI BssSI BstAPI
BstF5I BstNI BstUI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DraIII DsaI EaeI EarI EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MamI MboI MboII MnlI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV PflMI PleI PpuMI PstI
PvuII RsaI SapI Sau3AI Sau96I
ScrFI SfaNI SfcI SmaI SmlI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XcmI XmaCI XmaI XmnI