##################################
# NO CUT ENZYMES ( 67 enzymes) #
##################################
AccI AclI AflII AgeI AscI
AseI AsnI Asp700I AvrII BcgI
BfrI BlnI BsiEI BsiWI Bsp1407I
BspDI BsrGI BssSI Bst1107I BstBI
BstEII BstPI BstZ17I ClaI CpoI
DraI EagI EclXI Eco52I EcoO65I
EcoRI EcoT22I FseI HpaI KspI
MfeI MluI MunI NdeI NheI
NotI NruI NsiI NspV PacI
PaeR7I PinAI PmeI PshAI PshBI
Psp1406I PvuI RsrII SacII SalI
SapI ScaI SfiI SfuI SgrAI
SnaBI SpeI SspBI SwaI XbaI
XhoI XmnI
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
AatII 514 GACGTC GACGT^C
AccIII 99 TCCGGA T^CCGGA
AflIII 3163 ACATGT A^C[GA][CT]GT
AhdI 3057 GACCCTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 1184 AGCGCT AGC^GCT
AspEI 3057 GACCCTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 4884 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 111 TGCGCA TGC^GCA
BamHI 148 GGATCC G^GATCC
BbeI 239 GGCGCC GGCGC^C
BbrPI 2484 CACGTG CAC^GTG
BciVI 4243 GTATCC GTATCC(6/5)
BclI 2866 TGATCA T^GATCA
BsaAI 2484 CACGTG [CT]AC^GT[GA]
BsaBI 1951 GATCTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 99 TCCGGA T^CCGGA
BsmI 250 GCATTC GAATGC(1/-1)
BspEI 99 TCCGGA T^CCGGA
BspHI 3091 TCATGA T^CATGA
BspLU11I 3163 ACATGT A^CATGT
BssHII 435 GCGCGC G^CGCGC
DrdI 3447 GACCTGGCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 3057 GACCCTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 1184 AGCGCT AGC^GCT
EcoRV 1405 GATATC GAT^ATC
FbaI 2866 TGATCA T^GATCA
FspI 111 TGCGCA TGC^GCA
KasI 239 GGCGCC G^GCGCC
MamI 1951 GATCTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 99 TCCGGA T^CCGGA
NarI 239 GGCGCC GG^CGCC
NcoI 3616 CCATGG C^CATGG
PflFI 4884 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 2484 CACGTG CAC^GTG
PmlI 2484 CACGTG CAC^GTG
RcaI 3091 TCATGA T^CATGA
SexAI 728 ACCAGGT A^CC[AT]GGT
SfoI 239 GGCGCC GGC^GCC
SphI 2805 GCATGC GCATG^C
Tth111I 4884 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
Acc65I 2678 GGTACC G^GTACC
3745 GGTACC
AcsI 5027 AAATTT [GA]^AATT[CT]
5206 AAATTC
Alw44I 71 GTGCAC G^TGCAC
3877 GTGCAC
ApaLI 71 GTGCAC G^TGCAC
3877 GTGCAC
ApoI 5027 AAATTT [GA]^AATT[CT]
5206 AAATTC
Asp718I 2678 GGTACC G^GTACC
3745 GGTACC
BglII 1950 AGATCT A^GATCT
2603 AGATCT
BlpI 695 GCTCAGC GC^T[ACGT]AGC
1087 GCTGAGC
Bpu1102I 695 GCTCAGC GC^T[ACGT]AGC
1087 GCTGAGC
BsrBI 95 CCGCTC CCGCTC(-3/-3)
4529 GAGCGG
BsrDI 908 CATTGC GCAATG(2/0)
2479 CATTGC
CelII 695 GCTCAGC GC^T[ACGT]AGC
1087 GCTGAGC
HindIII 2764 AAGCTT A^AGCTT
4956 AAGCTT
KpnI 2678 GGTACC GGTAC^C
3745 GGTACC
SbfI 2323 CCTGCAGG CCTGCA^GG
3739 CCTGCAGG
Sse8387I 2323 CCTGCAGG CCTGCA^GG
3739 CCTGCAGG
SspI 3919 AATATT AAT^ATT
4290 AATATT
#########################################
# 3 OR MORE CUT ENZYMES ( 134 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI AocI ApaI AspHI
AvaI AvaII BalI BanI BanII
BbsI BbvCI BbvI BcnI BfaI
BglI BmyI BpmI BpuAI BsaHI
BsaI BsaJI BsaWI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmBI
BsmFI BsoBI Bsp1286I BspMI BsrFI
BsrI BssKI BstAPI BstF5I BstNI
BstUI BstXI BstYI Bsu36I Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DraIII DsaI EaeI EarI
Eco81I EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HincII HindII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NaeI NciI
NdeII NgoMIV NlaIII NlaIV NspI
PflMI PleI PpuMI PstI PvuII
RsaI SacI Sau3AI Sau96I ScrFI
SfaNI SfcI SmaI SmlI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XcmI XhoII XmaCI XmaI