##################################
# NO CUT ENZYMES ( 96 enzymes) #
##################################
Acc65I AclI AflIII AgeI AhdI
Alw44I Aor51HI ApaI ApaLI AscI
AseI AsnI Asp718I AspEI AspI
AvrII BamHI BbeI BclI BglI
BglII BlnI BlpI Bpu1102I BsiEI
BsiWI Bsp1407I BspDI BspLU11I BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstZ17I CelII ClaI
CpoI DraI EagI Eam1105I EclXI
Eco47III Eco52I EcoO65I EcoRV EcoT22I
FbaI FseI HaeII HindIII HpaI
KasI KpnI KspI MfeI MluI
MunI NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PmeI
PshBI Psp1406I PvuI RsrII SacII
SalI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SspBI SspI
SwaI Tth111I XbaI XhoI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
AatII 2096 GACGTC GACGT^C
AccI 18 GTAGAC GT^[AC][GT]AC
AccII 1126 CGCG CG^CG
AccIII 2007 TCCGGA T^CCGGA
AflII 2629 CTTAAG C^TTAAG
Asp700I 2605 GAAAAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII 1850 TGCGCA TGC^GCA
BalI 1563 TGGCCA TGG^CCA
BbrPI 2400 CACGTG CAC^GTG
BbvCI 9 CCTCAGC CCTCAGC(-5/-2)
BciVI 2509 GGATAC GTATCC(6/5)
BfrI 2629 CTTAAG C^TTAAG
BsaAI 2400 CACGTG [CT]AC^GT[GA]
BsaBI 1618 GATGAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 2007 TCCGGA [AT]^CCGG[AT]
BseAI 2007 TCCGGA T^CCGGA
BsmBI 2017 CGTCTC CGTCTC(1/5)
BspEI 2007 TCCGGA T^CCGGA
BspHI 1300 TCATGA T^CATGA
BsrBI 1258 CCGCTC CCGCTC(-3/-3)
BsrDI 1810 CATTGC GCAATG(2/0)
BsrFI 554 ACCGGC [GA]^CCGG[CT]
BstUI 1126 CGCG CG^CG
Cfr10I 554 ACCGGC [GA]^CCGG[CT]
DraIII 1556 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1799 GACTTTGCCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 1563 TGGCCA [CT]^GGCC[GA]
EcoNI 634 CCTGGAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 1077 GAATTC G^AATTC
FspI 1850 TGCGCA TGC^GCA
MamI 1618 GATGAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 1563 TGGCCA TGG^CCA
MroI 2007 TCCGGA T^CCGGA
MscI 1563 TGGCCA TGG^CCA
MvnI 1126 CGCG CG^CG
NspI 2260 GCATGC [GA]CATG^[CT]
PmaCI 2400 CACGTG CAC^GTG
PmlI 2400 CACGTG CAC^GTG
PshAI 2519 GACTCCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 1300 TCATGA T^CATGA
SacI 356 GAGCTC GAGCT^C
SapI 1581 GAAGAGC GCTCTTC(1/4)
SbfI 1357 CCTGCAGG CCTGCA^GG
ScaI 579 AGTACT AGT^ACT
SphI 2260 GCATGC GCATG^C
Sse8387I 1357 CCTGCAGG CCTGCA^GG
TaqI 2425 TCGA T^CGA
TthHB8I 2425 TCGA T^CGA
XmnI 2605 GAAAAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AcsI 1077 GAATTC [GA]^AATT[CT]
2608 AAATTT
AocI 271 CCTGAGG CC^T[ACGT]AGG
1894 CCTGAGG
ApoI 1077 GAATTC [GA]^AATT[CT]
2608 AAATTT
AspHI 356 GAGCTC G[AT]GC[AT]^C
1265 GTGCTC
AvaI 23 CCCGAG C^[CT]CG[GA]G
2020 CTCGGG
BbsI 1456 GAAGAC GAAGAC(2/6)
2219 GTCTTC
BcgI 538 CGAGTTCGGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2195 CGACAATCCTGC
BcnI 1192 CCGGG CC^[GC]GG
2170 CCGGG
BpuAI 1456 GAAGAC GAAGAC(2/6)
2219 GTCTTC
BseRI 638 GAGGAG GAGGAG(10/8)
1750 GAGGAG
BsiHKAI 356 GAGCTC G[AT]GC[AT]^C
1265 GTGCTC
BsmI 2177 GAATGC GAATGC(1/-1)
1511 GCATTC
BsoBI 23 CCCGAG C^[CT]CG[GA]G
2020 CTCGGG
BstYI 1105 AGATCC [GA]^GATC[CT]
1464 AGATCC
Bsu36I 271 CCTGAGG CC^T[ACGT]AGG
1894 CCTGAGG
CfoI 51 GCGC GCG^C
1851 GCGC
Eco81I 271 CCTGAGG CC^T[ACGT]AGG
1894 CCTGAGG
HhaI 51 GCGC GCG^C
1851 GCGC
HinP1I 51 GCGC G^CGC
1851 GCGC
MflI 1105 AGATCC [GA]^GATC[CT]
1464 AGATCC
NciI 1192 CCGGG CC^[GC]GG
2170 CCGGG
NcoI 906 CCATGG C^CATGG
2541 CCATGG
PvuII 590 CAGCTG CAG^CTG
2182 CAGCTG
SexAI 1054 ACCTGGT A^CC[AT]GGT
1557 ACCTGGT
SmlI 486 CTCAAG C^T[CT][GA]AG
2629 CTTAAG
XcmI 421 CCAGGTCAACACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
777 CCAAGGACAAGCTGG
XhoII 1105 AGATCC [GA]^GATC[CT]
1464 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 86 enzymes) #
#########################################
AciI AcyI AfaI AluI AlwI
AlwNI AvaII BanI BanII BbvI
BfaI BmyI BpmI BsaHI BsaI
BsaJI BsgI BsiYI BslI BsmAI
BsmFI Bsp1286I BspMI BsrI BssKI
BstF5I BstNI BstXI Cac8I Cfr13I
DdeI DpnII DraII DsaI EarI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HgaI Hin1I
HincII HindII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MnlI MseI MslI
MspA1I MspI MvaI MwoI NdeII
NlaIII NlaIV PflMI PleI PpuMI
PstI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI StuI StyI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
Van91I