##################################
# NO CUT ENZYMES ( 53 enzymes) #
##################################
AatII AccI AflII AgeI AscI
AspI BamHI BciVI BclI BfrI
BsaBI BsiEI BsiWI BsmBI BspDI
BssHII Bst1107I BstBI BstZ17I ClaI
EagI EclXI Eco52I EcoRI EcoRV
EcoT22I FbaI FseI HpaI MamI
MluI NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PmeI PvuI SalI SbfI SfuI
SgrAI SnaBI SpeI Sse8387I SspI
SwaI Tth111I XhoI
###################################
# ONE CUT ENZYMES ( 34 enzymes) #
###################################
AccIII 432 TCCGGA T^CCGGA
AclI 2949 AACGTT AA^CGTT
Aor51HI 2561 AGCGCT AGC^GCT
AseI 5888 ATTAAT AT^TAAT
AsnI 5888 ATTAAT AT^TAAT
AviII 3024 TGCGCA TGC^GCA
BlpI 2982 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 2982 GCTGAGC GC^T[ACGT]AGC
BseAI 432 TCCGGA T^CCGGA
BspEI 432 TCCGGA T^CCGGA
BstEII 2667 GGTGACC G^GT[ACGT]ACC
BstPI 2667 GGTGACC G^GT[ACGT]ACC
CelII 2982 GCTGAGC GC^T[ACGT]AGC
CpoI 2396 CGGACCG CG^G[AT]CCG
Eco47III 2561 AGCGCT AGC^GCT
EcoO65I 2667 GGTGACC G^GT[ACGT]ACC
FspI 3024 TGCGCA TGC^GCA
HincII 845 GTCAAC GT[CT]^[GA]AC
HindII 845 GTCAAC GT[CT]^[GA]AC
KspI 2393 CCGCGG CCGC^GG
MfeI 4519 CAATTG C^AATTG
MroI 432 TCCGGA T^CCGGA
MunI 4519 CAATTG C^AATTG
PshAI 2038 GACAAACGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 5888 ATTAAT AT^TAAT
Psp1406I 2949 AACGTT AA^CGTT
RsrII 2396 CGGACCG CG^G[AT]CCG
SacII 2393 CCGCGG CCGC^GG
ScaI 1982 AGTACT AGT^ACT
SfiI 3824 GGCCAGCGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 69 CCCGGG CCC^GGG
XbaI 4280 TCTAGA T^CTAGA
XmaCI 69 CCCGGG C^CCGGG
XmaI 69 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
Acc65I 1372 GGTACC G^GTACC
2414 GGTACC
AocI 3124 CCTAAGG CC^T[ACGT]AGG
5499 CCTAAGG
Asp718I 1372 GGTACC G^GTACC
2414 GGTACC
BbrPI 449 CACGTG CAC^GTG
1594 CACGTG
BbvCI 4635 CCTCAGC CCTCAGC(-5/-2)
1381 GCTGAGG
BcgI 5903 CGAACTAACTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
3088 GCAAGCACCTCG
BsaWI 385 TCCGGT [AT]^CCGG[AT]
432 TCCGGA
Bsp1407I 1895 TGTACA T^GTACA
5893 TGTACA
BspHI 1853 TCATGA T^CATGA
4377 TCATGA
BspLU11I 1442 ACATGT A^CATGT
3620 ACATGT
BsrBI 2014 CCGCTC CCGCTC(-3/-3)
52 GAGCGG
BsrDI 4366 CATTGC GCAATG(2/0)
4581 CATTGC
BsrFI 1042 GCCGGC [GA]^CCGG[CT]
2482 GCCGGC
BsrGI 1895 TGTACA T^GTACA
5893 TGTACA
BssSI 3206 CACGAG CACGAG(-5/-1)
334 CTCGTG
Bsu36I 3124 CCTAAGG CC^T[ACGT]AGG
5499 CCTAAGG
Cfr10I 1042 GCCGGC [GA]^CCGG[CT]
2482 GCCGGC
DraIII 44 CACGGAGTG CAC[ACGT][ACGT][ACGT]^GTG
1909 CACCTGGTG
DrdI 1002 GACGCGCTCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1857 GACGCACCTGTC
Eco81I 3124 CCTAAGG CC^T[ACGT]AGG
5499 CCTAAGG
HindIII 1147 AAGCTT A^AGCTT
4397 AAGCTT
KpnI 1372 GGTACC GGTAC^C
2414 GGTACC
NaeI 1042 GCCGGC GCC^GGC
2482 GCCGGC
NcoI 170 CCATGG C^CATGG
4202 CCATGG
NdeI 3305 CATATG CA^TATG
5051 CATATG
NgoMIV 1042 GCCGGC G^CCGGC
2482 GCCGGC
PmaCI 449 CACGTG CAC^GTG
1594 CACGTG
PmlI 449 CACGTG CAC^GTG
1594 CACGTG
RcaI 1853 TCATGA T^CATGA
4377 TCATGA
SexAI 1910 ACCTGGT A^CC[AT]GGT
2917 ACCAGGT
SmlI 620 CTCAAG C^T[CT][GA]AG
836 CTCAAG
SspBI 1895 TGTACA T^GTACA
5893 TGTACA
StuI 541 AGGCCT AGG^CCT
4681 AGGCCT
#########################################
# 3 OR MORE CUT ENZYMES ( 138 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflIII AhdI AluI Alw44I AlwI
AlwNI ApaI ApaLI ApoI Asp700I
AspEI AspHI AvaI AvaII AvrII
BalI BanI BanII BbeI BbsI
BbvI BcnI BfaI BglI BglII
BlnI BmyI BpmI BpuAI BsaAI
BsaHI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI BsoBI Bsp1286I BspMI BsrI
BssKI BstAPI BstF5I BstNI BstUI
BstXI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraI DraII DsaI
EaeI Eam1105I EarI EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI KasI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NarI
NciI NdeII NlaIII NlaIV NspI
PflMI PleI PpuMI PstI PvuII
RsaI SacI SapI Sau3AI Sau96I
ScrFI SfaNI SfcI SfoI SphI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XcmI XhoII XmnI