################################## # NO CUT ENZYMES ( 53 enzymes) # ################################## AatII AccI AflII AgeI AscI AspI BamHI BciVI BclI BfrI BsaBI BsiEI BsiWI BsmBI BspDI BssHII Bst1107I BstBI BstZ17I ClaI EagI EclXI Eco52I EcoRI EcoRV EcoT22I FbaI FseI HpaI MamI MluI NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmeI PvuI SalI SbfI SfuI SgrAI SnaBI SpeI Sse8387I SspI SwaI Tth111I XhoI ################################### # ONE CUT ENZYMES ( 34 enzymes) # ################################### AccIII 432 TCCGGA T^CCGGA AclI 2949 AACGTT AA^CGTT Aor51HI 2561 AGCGCT AGC^GCT AseI 5888 ATTAAT AT^TAAT AsnI 5888 ATTAAT AT^TAAT AviII 3024 TGCGCA TGC^GCA BlpI 2982 GCTGAGC GC^T[ACGT]AGC Bpu1102I 2982 GCTGAGC GC^T[ACGT]AGC BseAI 432 TCCGGA T^CCGGA BspEI 432 TCCGGA T^CCGGA BstEII 2667 GGTGACC G^GT[ACGT]ACC BstPI 2667 GGTGACC G^GT[ACGT]ACC CelII 2982 GCTGAGC GC^T[ACGT]AGC CpoI 2396 CGGACCG CG^G[AT]CCG Eco47III 2561 AGCGCT AGC^GCT EcoO65I 2667 GGTGACC G^GT[ACGT]ACC FspI 3024 TGCGCA TGC^GCA HincII 845 GTCAAC GT[CT]^[GA]AC HindII 845 GTCAAC GT[CT]^[GA]AC KspI 2393 CCGCGG CCGC^GG MfeI 4519 CAATTG C^AATTG MroI 432 TCCGGA T^CCGGA MunI 4519 CAATTG C^AATTG PshAI 2038 GACAAACGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PshBI 5888 ATTAAT AT^TAAT Psp1406I 2949 AACGTT AA^CGTT RsrII 2396 CGGACCG CG^G[AT]CCG SacII 2393 CCGCGG CCGC^GG ScaI 1982 AGTACT AGT^ACT SfiI 3824 GGCCAGCGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SmaI 69 CCCGGG CCC^GGG XbaI 4280 TCTAGA T^CTAGA XmaCI 69 CCCGGG C^CCGGG XmaI 69 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 33 enzymes) # ################################### Acc65I 1372 GGTACC G^GTACC 2414 GGTACC AocI 3124 CCTAAGG CC^T[ACGT]AGG 5499 CCTAAGG Asp718I 1372 GGTACC G^GTACC 2414 GGTACC BbrPI 449 CACGTG CAC^GTG 1594 CACGTG BbvCI 4635 CCTCAGC CCTCAGC(-5/-2) 1381 GCTGAGG BcgI 5903 CGAACTAACTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 3088 GCAAGCACCTCG BsaWI 385 TCCGGT [AT]^CCGG[AT] 432 TCCGGA Bsp1407I 1895 TGTACA T^GTACA 5893 TGTACA BspHI 1853 TCATGA T^CATGA 4377 TCATGA BspLU11I 1442 ACATGT A^CATGT 3620 ACATGT BsrBI 2014 CCGCTC CCGCTC(-3/-3) 52 GAGCGG BsrDI 4366 CATTGC GCAATG(2/0) 4581 CATTGC BsrFI 1042 GCCGGC [GA]^CCGG[CT] 2482 GCCGGC BsrGI 1895 TGTACA T^GTACA 5893 TGTACA BssSI 3206 CACGAG CACGAG(-5/-1) 334 CTCGTG Bsu36I 3124 CCTAAGG CC^T[ACGT]AGG 5499 CCTAAGG Cfr10I 1042 GCCGGC [GA]^CCGG[CT] 2482 GCCGGC DraIII 44 CACGGAGTG CAC[ACGT][ACGT][ACGT]^GTG 1909 CACCTGGTG DrdI 1002 GACGCGCTCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1857 GACGCACCTGTC Eco81I 3124 CCTAAGG CC^T[ACGT]AGG 5499 CCTAAGG HindIII 1147 AAGCTT A^AGCTT 4397 AAGCTT KpnI 1372 GGTACC GGTAC^C 2414 GGTACC NaeI 1042 GCCGGC GCC^GGC 2482 GCCGGC NcoI 170 CCATGG C^CATGG 4202 CCATGG NdeI 3305 CATATG CA^TATG 5051 CATATG NgoMIV 1042 GCCGGC G^CCGGC 2482 GCCGGC PmaCI 449 CACGTG CAC^GTG 1594 CACGTG PmlI 449 CACGTG CAC^GTG 1594 CACGTG RcaI 1853 TCATGA T^CATGA 4377 TCATGA SexAI 1910 ACCTGGT A^CC[AT]GGT 2917 ACCAGGT SmlI 620 CTCAAG C^T[CT][GA]AG 836 CTCAAG SspBI 1895 TGTACA T^GTACA 5893 TGTACA StuI 541 AGGCCT AGG^CCT 4681 AGGCCT ######################################### # 3 OR MORE CUT ENZYMES ( 138 enzymes) # ######################################### AccII AciI AcsI AcyI AfaI AflIII AhdI AluI Alw44I AlwI AlwNI ApaI ApaLI ApoI Asp700I AspEI AspHI AvaI AvaII AvrII BalI BanI BanII BbeI BbsI BbvI BcnI BfaI BglI BglII BlnI BmyI BpmI BpuAI BsaAI BsaHI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I BspMI BsrI BssKI BstAPI BstF5I BstNI BstUI BstXI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraI DraII DsaI EaeI Eam1105I EarI EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI KasI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NarI NciI NdeII NlaIII NlaIV NspI PflMI PleI PpuMI PstI PvuII RsaI SacI SapI Sau3AI Sau96I ScrFI SfaNI SfcI SfoI SphI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI XhoII XmnI