##################################
# NO CUT ENZYMES ( 62 enzymes) #
##################################
AclI AgeI Alw44I ApaLI AscI
AseI AsnI Asp700I BcgI BciVI
BclI BsaBI BsiWI BsmI BspDI
BspLU11I BssHII Bst1107I BstBI BstZ17I
ClaI CpoI EcoRI EcoRV EcoT22I
FbaI FseI HincII HindII HpaI
MamI MfeI MluI MunI NdeI
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PinAI PmeI
PshBI Psp1406I PvuI RsrII SalI
SexAI SfiI SfuI SgrAI SnaBI
SpeI SphI SspI SwaI XbaI
XhoI XmnI
###################################
# ONE CUT ENZYMES ( 25 enzymes) #
###################################
AatII 1706 GACGTC GACGT^C
Acc65I 4439 GGTACC G^GTACC
AhdI 1154 GACCCACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp718I 4439 GGTACC G^GTACC
AspEI 1154 GACCCACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 744 TGCGCA TGC^GCA
BbsI 5279 GAAGAC GAAGAC(2/6)
BpuAI 5279 GAAGAC GAAGAC(2/6)
Bsp1407I 3309 TGTACA T^GTACA
BspHI 1602 TCATGA T^CATGA
BsrBI 4680 CCGCTC CCGCTC(-3/-3)
BsrGI 3309 TGTACA T^GTACA
DraIII 1363 CACTATGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1706 GACGTCGATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 1154 GACCCACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FspI 744 TGCGCA TGC^GCA
KpnI 4439 GGTACC GGTAC^C
KspI 4388 CCGCGG CCGC^GG
RcaI 1602 TCATGA T^CATGA
SacII 4388 CCGCGG CCGC^GG
ScaI 2667 AGTACT AGT^ACT
SmaI 5551 CCCGGG CCC^GGG
SspBI 3309 TGTACA T^GTACA
XmaCI 5551 CCCGGG C^CCGGG
XmaI 5551 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 35 enzymes) #
###################################
AccIII 222 TCCGGA T^CCGGA
1749 TCCGGA
AcsI 284 GAATTT [GA]^AATT[CT]
2287 AAATTT
AflII 2130 CTTAAG C^TTAAG
2192 CTTAAG
AflIII 827 ACGTGT A^C[GA][CT]GT
1797 ACGTGT
Aor51HI 560 AGCGCT AGC^GCT
4414 AGCGCT
ApoI 284 GAATTT [GA]^AATT[CT]
2287 AAATTT
AspI 1742 GACAACGTC GAC[ACGT]^[ACGT][ACGT]GTC
2263 GACCCTGTC
BamHI 570 GGATCC G^GATCC
898 GGATCC
BbeI 1588 GGCGCC GGCGC^C
1933 GGCGCC
BbrPI 1796 CACGTG CAC^GTG
3931 CACGTG
BfrI 2130 CTTAAG C^TTAAG
2192 CTTAAG
BsaWI 222 TCCGGA [AT]^CCGG[AT]
1749 TCCGGA
BseAI 222 TCCGGA T^CCGGA
1749 TCCGGA
BsmBI 2886 CGTCTC CGTCTC(1/5)
5461 CGTCTC
BspEI 222 TCCGGA T^CCGGA
1749 TCCGGA
BstEII 4004 GGTTACC G^GT[ACGT]ACC
5132 GGTGACC
BstPI 4004 GGTTACC G^GT[ACGT]ACC
5132 GGTGACC
DraI 2139 TTTAAA TTT^AAA
2291 TTTAAA
Eco47III 560 AGCGCT AGC^GCT
4414 AGCGCT
EcoO65I 4004 GGTTACC G^GT[ACGT]ACC
5132 GGTGACC
HindIII 1189 AAGCTT A^AGCTT
2127 AAGCTT
KasI 1588 GGCGCC G^GCGCC
1933 GGCGCC
MroI 222 TCCGGA T^CCGGA
1749 TCCGGA
NarI 1588 GGCGCC GG^CGCC
1933 GGCGCC
NcoI 2852 CCATGG C^CATGG
5079 CCATGG
PflFI 1742 GACAACGTC GAC[ACGT]^[ACGT][ACGT]GTC
2263 GACCCTGTC
PmaCI 1796 CACGTG CAC^GTG
3931 CACGTG
PmlI 1796 CACGTG CAC^GTG
3931 CACGTG
PshAI 420 GACCCTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
1702 GACGGACGTC
SacI 3208 GAGCTC GAGCT^C
4630 GAGCTC
SbfI 2902 CCTGCAGG CCTGCA^GG
4380 CCTGCAGG
SfoI 1588 GGCGCC GGC^GCC
1933 GGCGCC
Sse8387I 2902 CCTGCAGG CCTGCA^GG
4380 CCTGCAGG
TfiI 2555 GATTC G^A[AT]TC
4191 GAATC
Tth111I 1742 GACAACGTC GAC[ACGT]^[ACGT][ACGT]GTC
2263 GACCCTGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 136 enzymes) #
#########################################
AccI AccII AciI AcyI AfaI
AluI AlwI AlwNI AocI ApaI
AspHI AvaI AvaII AvrII BalI
BanI BanII BbvCI BbvI BcnI
BfaI BglI BglII BlnI BlpI
BmyI BpmI Bpu1102I BsaAI BsaHI
BsaI BsaJI BseRI BsgI BsiEI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrDI BsrFI
BsrI BssKI BssSI BstAPI BstF5I
BstNI BstUI BstXI BstYI Bsu36I
Cac8I CelII CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
EagI EarI EclXI Eco52I Eco81I
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NaeI NciI NdeII NgoMIV NlaIII
NlaIV PflMI PleI PpuMI PstI
PvuII RsaI SapI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StuI
StyI TaqI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XcmI
XhoII