##################################
# NO CUT ENZYMES (   54 enzymes) #
##################################

AatII       AclI        AgeI        AhdI        Aor51HI     
AscI        AseI        AsnI        AspEI       AvrII       
BbvCI       BciVI       BclI        BlnI        BsaBI       
BsiWI       BsmBI       Bsp1407I    BspLU11I    BsrGI       
BstBI       DraI        DrdI        Eam1105I    Eco47III    
EcoRI       EcoT22I     FbaI        FseI        HindIII     
HpaI        MamI        NdeI        NheI        NotI        
NruI        NsiI        NspV        PacI        PinAI       
PmeI        PshAI       PshBI       Psp1406I    PvuI        
SalI        SfiI        SfuI        SnaBI       SpeI        
SspBI       SspI        SwaI        XbaI        


###################################
# ONE CUT ENZYMES (   48 enzymes) #
###################################

AccIII       3468  TCCGGA               T^CCGGA
AcsI         5402  GAATTT               [GA]^AATT[CT]
AflII        4321  CTTAAG               C^TTAAG
Alw44I       2532  GTGCAC               G^TGCAC
AocI         1783  CCTGAGG              CC^T[ACGT]AGG
ApaLI        2532  GTGCAC               G^TGCAC
ApoI         5402  GAATTT               [GA]^AATT[CT]
Asp700I      3684  GAAAGACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI         3625  GACCTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AviII        3062  TGCGCA               TGC^GCA
BbrPI        3754  CACGTG               CAC^GTG
BcgI         2243  GCACAGGCCTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI         4321  CTTAAG               C^TTAAG
BglII        4650  AGATCT               A^GATCT
BsaAI        3754  CACGTG               [CT]AC^GT[GA]
BseAI        3468  TCCGGA               T^CCGGA
BspDI        1947  ATCGAT               AT^CGAT
BspEI        3468  TCCGGA               T^CCGGA
BspHI        4761  TCATGA               T^CATGA
BssHII        411  GCGCGC               G^CGCGC
BssSI        5052  CACGAG               CACGAG(-5/-1)
Bst1107I     1150  GTATAC               GTA^TAC
BstEII       5057  GGTGACC              G^GT[ACGT]ACC
BstPI        5057  GGTGACC              G^GT[ACGT]ACC
BstZ17I      1150  GTATAC               GTA^TAC
Bsu36I       1783  CCTGAGG              CC^T[ACGT]AGG
ClaI         1947  ATCGAT               AT^CGAT
CpoI         4740  CGGTCCG              CG^G[AT]CCG
Eco81I       1783  CCTGAGG              CC^T[ACGT]AGG
EcoO65I      5057  GGTGACC              G^GT[ACGT]ACC
EcoRV        2644  GATATC               GAT^ATC
FspI         3062  TGCGCA               TGC^GCA
MfeI         4426  CAATTG               C^AATTG
MluI          437  ACGCGT               A^CGCGT
MroI         3468  TCCGGA               T^CCGGA
MunI         4426  CAATTG               C^AATTG
PaeR7I        686  CTCGAG               C^TCGAG
PflFI        3625  GACCTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI        3754  CACGTG               CAC^GTG
PmlI         3754  CACGTG               CAC^GTG
RcaI         4761  TCATGA               T^CATGA
RsrII        4740  CGGTCCG              CG^G[AT]CCG
SacI         1052  GAGCTC               GAGCT^C
ScaI         3473  AGTACT               AGT^ACT
SgrAI          60  CGCCGGTG             C[GA]^CCGG[CT]G
Tth111I      3625  GACCTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XhoI          686  CTCGAG               C^TCGAG
XmnI         3684  GAAAGACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   13 enzymes) #
###################################

Acc65I       1499  GGTACC               G^GTACC
             4404  GGTACC              
AflIII        437  ACGCGT               A^C[GA][CT]GT
             3755  ACGTGT              
Asp718I      1499  GGTACC               G^GTACC
             4404  GGTACC              
BamHI        1218  GGATCC               G^GATCC
             3218  GGATCC              
BsaWI        1884  ACCGGA               [AT]^CCGG[AT]
             3468  TCCGGA              
BstAPI       1929  GCAGGAGGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             2390  GCAGCCGTTGC         
HincII        853  GTCAAC               GT[CT]^[GA]AC
             4266  GTCAAC              
HindII        853  GTCAAC               GT[CT]^[GA]AC
             4266  GTCAAC              
KpnI         1499  GGTACC               GGTAC^C
             4404  GGTACC              
MaeII        1466  ACGT                 A^CGT
             3755  ACGT                
SbfI         3949  CCTGCAGG             CCTGCA^GG
             4820  CCTGCAGG            
SphI         3645  GCATGC               GCATG^C
             4606  GCATGC              
Sse8387I     3949  CCTGCAGG             CCTGCA^GG
             4820  CCTGCAGG            


#########################################
# 3 OR MORE CUT ENZYMES (  143 enzymes) #
#########################################

AccI        AccII       AciI        AcyI        AfaI        
AluI        AlwI        AlwNI       ApaI        AspHI       
AvaI        AvaII       BalI        BanI        BanII       
BbeI        BbsI        BbvI        BcnI        BfaI        
BglI        BlpI        BmyI        BpmI        Bpu1102I    
BpuAI       BsaHI       BsaI        BsaJI       BseRI       
BsgI        BsiEI       BsiHKAI     BsiYI       BslI        
BsmAI       BsmFI       BsmI        BsoBI       Bsp1286I    
BspMI       BsrBI       BsrDI       BsrFI       BsrI        
BssKI       BstF5I      BstNI       BstUI       BstXI       
BstYI       Cac8I       CelII       CfoI        Cfr10I      
Cfr13I      DdeI        DpnII       DraII       DraIII      
DsaI        EaeI        EagI        EarI        EclXI       
Eco52I      EcoNI       EcoO109I    EcoRII      EcoT14I     
FauI        Fnu4HI      FokI        HaeII       HaeIII      
HapII       HgaI        HhaI        Hin1I       HinP1I      
HinfI       HpaII       HphI        ItaI        KasI        
Ksp632I     KspI        MaeI        MaeIII      MboI        
MboII       MflI        MluNI       MnlI        MscI        
MseI        MslI        MspA1I      MspI        MvaI        
MvnI        MwoI        NaeI        NarI        NciI        
NcoI        NdeII       NgoMIV      NlaIII      NlaIV       
NspI        PflMI       PleI        PpuMI       PstI        
PvuII       RsaI        SacII       SapI        Sau3AI      
Sau96I      ScrFI       SexAI       SfaNI       SfcI        
SfoI        SmaI        SmlI        StuI        StyI        
TaqI        TfiI        Tru9I       TseI        Tsp45I      
Tsp509I     TspRI       TthHB8I     Van91I      XcmI        
XhoII       XmaCI       XmaI