##################################
# NO CUT ENZYMES ( 54 enzymes) #
##################################
AatII AclI AgeI AhdI Aor51HI
AscI AseI AsnI AspEI AvrII
BbvCI BciVI BclI BlnI BsaBI
BsiWI BsmBI Bsp1407I BspLU11I BsrGI
BstBI DraI DrdI Eam1105I Eco47III
EcoRI EcoT22I FbaI FseI HindIII
HpaI MamI NdeI NheI NotI
NruI NsiI NspV PacI PinAI
PmeI PshAI PshBI Psp1406I PvuI
SalI SfiI SfuI SnaBI SpeI
SspBI SspI SwaI XbaI
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
AccIII 3468 TCCGGA T^CCGGA
AcsI 5402 GAATTT [GA]^AATT[CT]
AflII 4321 CTTAAG C^TTAAG
Alw44I 2532 GTGCAC G^TGCAC
AocI 1783 CCTGAGG CC^T[ACGT]AGG
ApaLI 2532 GTGCAC G^TGCAC
ApoI 5402 GAATTT [GA]^AATT[CT]
Asp700I 3684 GAAAGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 3625 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 3062 TGCGCA TGC^GCA
BbrPI 3754 CACGTG CAC^GTG
BcgI 2243 GCACAGGCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 4321 CTTAAG C^TTAAG
BglII 4650 AGATCT A^GATCT
BsaAI 3754 CACGTG [CT]AC^GT[GA]
BseAI 3468 TCCGGA T^CCGGA
BspDI 1947 ATCGAT AT^CGAT
BspEI 3468 TCCGGA T^CCGGA
BspHI 4761 TCATGA T^CATGA
BssHII 411 GCGCGC G^CGCGC
BssSI 5052 CACGAG CACGAG(-5/-1)
Bst1107I 1150 GTATAC GTA^TAC
BstEII 5057 GGTGACC G^GT[ACGT]ACC
BstPI 5057 GGTGACC G^GT[ACGT]ACC
BstZ17I 1150 GTATAC GTA^TAC
Bsu36I 1783 CCTGAGG CC^T[ACGT]AGG
ClaI 1947 ATCGAT AT^CGAT
CpoI 4740 CGGTCCG CG^G[AT]CCG
Eco81I 1783 CCTGAGG CC^T[ACGT]AGG
EcoO65I 5057 GGTGACC G^GT[ACGT]ACC
EcoRV 2644 GATATC GAT^ATC
FspI 3062 TGCGCA TGC^GCA
MfeI 4426 CAATTG C^AATTG
MluI 437 ACGCGT A^CGCGT
MroI 3468 TCCGGA T^CCGGA
MunI 4426 CAATTG C^AATTG
PaeR7I 686 CTCGAG C^TCGAG
PflFI 3625 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 3754 CACGTG CAC^GTG
PmlI 3754 CACGTG CAC^GTG
RcaI 4761 TCATGA T^CATGA
RsrII 4740 CGGTCCG CG^G[AT]CCG
SacI 1052 GAGCTC GAGCT^C
ScaI 3473 AGTACT AGT^ACT
SgrAI 60 CGCCGGTG C[GA]^CCGG[CT]G
Tth111I 3625 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 686 CTCGAG C^TCGAG
XmnI 3684 GAAAGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 13 enzymes) #
###################################
Acc65I 1499 GGTACC G^GTACC
4404 GGTACC
AflIII 437 ACGCGT A^C[GA][CT]GT
3755 ACGTGT
Asp718I 1499 GGTACC G^GTACC
4404 GGTACC
BamHI 1218 GGATCC G^GATCC
3218 GGATCC
BsaWI 1884 ACCGGA [AT]^CCGG[AT]
3468 TCCGGA
BstAPI 1929 GCAGGAGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2390 GCAGCCGTTGC
HincII 853 GTCAAC GT[CT]^[GA]AC
4266 GTCAAC
HindII 853 GTCAAC GT[CT]^[GA]AC
4266 GTCAAC
KpnI 1499 GGTACC GGTAC^C
4404 GGTACC
MaeII 1466 ACGT A^CGT
3755 ACGT
SbfI 3949 CCTGCAGG CCTGCA^GG
4820 CCTGCAGG
SphI 3645 GCATGC GCATG^C
4606 GCATGC
Sse8387I 3949 CCTGCAGG CCTGCA^GG
4820 CCTGCAGG
#########################################
# 3 OR MORE CUT ENZYMES ( 143 enzymes) #
#########################################
AccI AccII AciI AcyI AfaI
AluI AlwI AlwNI ApaI AspHI
AvaI AvaII BalI BanI BanII
BbeI BbsI BbvI BcnI BfaI
BglI BlpI BmyI BpmI Bpu1102I
BpuAI BsaHI BsaI BsaJI BseRI
BsgI BsiEI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI BsoBI Bsp1286I
BspMI BsrBI BsrDI BsrFI BsrI
BssKI BstF5I BstNI BstUI BstXI
BstYI Cac8I CelII CfoI Cfr10I
Cfr13I DdeI DpnII DraII DraIII
DsaI EaeI EagI EarI EclXI
Eco52I EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI KasI
Ksp632I KspI MaeI MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NaeI NarI NciI
NcoI NdeII NgoMIV NlaIII NlaIV
NspI PflMI PleI PpuMI PstI
PvuII RsaI SacII SapI Sau3AI
Sau96I ScrFI SexAI SfaNI SfcI
SfoI SmaI SmlI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XcmI
XhoII XmaCI XmaI