##################################
# NO CUT ENZYMES ( 57 enzymes) #
##################################
Acc65I AccIII AflII AgeI AhdI
Alw44I Aor51HI ApaLI AscI Asp700I
Asp718I AspEI AvrII BfrI BlnI
BsaI BseAI BsiWI Bsp1407I BspEI
BsrGI BssHII BssSI Bst1107I BstZ17I
CpoI DrdI Eam1105I Eco47III FseI
HindIII HpaI KpnI MfeI MluI
MroI MunI NdeI NheI NotI
PacI PaeR7I PinAI PmeI PvuI
RsrII SalI ScaI SfiI SgrAI
SnaBI SphI SspBI SspI XbaI
XhoI XmnI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
AatII 1886 GACGTC GACGT^C
AclI 866 AACGTT AA^CGTT
AocI 1528 CCTCAGG CC^T[ACGT]AGG
ApaI 3116 GGGCCC GGGCC^C
AviII 2817 TGCGCA TGC^GCA
BbeI 3557 GGCGCC GGCGC^C
BcgI 598 CGAGATTGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 3274 GTATCC GTATCC(6/5)
BglI 94 GCCCCGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsmBI 5156 GAGACG CGTCTC(1/5)
BsrDI 3438 GCAATG GCAATG(2/0)
BstBI 2735 TTCGAA TT^CGAA
BstEII 3853 GGTGACC G^GT[ACGT]ACC
BstPI 3853 GGTGACC G^GT[ACGT]ACC
Bsu36I 1528 CCTCAGG CC^T[ACGT]AGG
DraIII 4934 CACCAGGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco81I 1528 CCTCAGG CC^T[ACGT]AGG
EcoNI 5062 CCTCCTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 3853 GGTGACC G^GT[ACGT]ACC
EcoT22I 4019 ATGCAT ATGCA^T
FspI 2817 TGCGCA TGC^GCA
HaeII 3557 GGCGCC [GA]GCGC^[CT]
HincII 683 GTTGAC GT[CT]^[GA]AC
HindII 683 GTTGAC GT[CT]^[GA]AC
KasI 3557 GGCGCC G^GCGCC
KspI 1429 CCGCGG CCGC^GG
NarI 3557 GGCGCC GG^CGCC
NsiI 4019 ATGCAT ATGCA^T
NspV 2735 TTCGAA TT^CGAA
PshAI 5584 GACACCCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 866 AACGTT AA^CGTT
SacII 1429 CCGCGG CCGC^GG
SbfI 5092 CCTGCAGG CCTGCA^GG
SfoI 3557 GGCGCC GGC^GCC
SfuI 2735 TTCGAA TT^CGAA
SpeI 2 ACTAGT A^CTAGT
Sse8387I 5092 CCTGCAGG CCTGCA^GG
SwaI 3637 ATTTAAAT ATTT^AAAT
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AccI 3980 GTCTAC GT^[AC][GT]AC
5410 GTCTAC
AseI 511 ATTAAT AT^TAAT
1858 ATTAAT
AsnI 511 ATTAAT AT^TAAT
1858 ATTAAT
AspI 5072 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
5288 GACTCTGTC
BamHI 1425 GGATCC G^GATCC
3461 GGATCC
BbrPI 3599 CACGTG CAC^GTG
5117 CACGTG
BfaI 3 CTAG C^TAG
4847 CTAG
BglII 3074 AGATCT A^GATCT
4789 AGATCT
BlpI 1696 GCTGAGC GC^T[ACGT]AGC
5381 GCTGAGC
Bpu1102I 1696 GCTGAGC GC^T[ACGT]AGC
5381 GCTGAGC
BsiEI 281 CGGCCG CG[GA][CT]^CG
4975 CGGCCG
BsmI 12 GAATGC GAATGC(1/-1)
3525 GCATTC
BspDI 1625 ATCGAT AT^CGAT
4814 ATCGAT
CelII 1696 GCTGAGC GC^T[ACGT]AGC
5381 GCTGAGC
ClaI 1625 ATCGAT AT^CGAT
4814 ATCGAT
EagI 281 CGGCCG C^GGCCG
4975 CGGCCG
EclXI 281 CGGCCG C^GGCCG
4975 CGGCCG
Eco52I 281 CGGCCG C^GGCCG
4975 CGGCCG
EcoRV 331 GATATC GAT^ATC
3746 GATATC
MaeI 3 CTAG C^TAG
4847 CTAG
NruI 33 TCGCGA TCG^CGA
4906 TCGCGA
PflFI 5072 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
5288 GACTCTGTC
PmaCI 3599 CACGTG CAC^GTG
5117 CACGTG
PmlI 3599 CACGTG CAC^GTG
5117 CACGTG
PshBI 511 ATTAAT AT^TAAT
1858 ATTAAT
SapI 2312 GAAGAGC GCTCTTC(1/4)
2119 GCTCTTC
SmaI 3513 CCCGGG CCC^GGG
5461 CCCGGG
Tth111I 5072 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
5288 GACTCTGTC
XmaCI 3513 CCCGGG C^CCGGG
5461 CCCGGG
XmaI 3513 CCCGGG C^CCGGG
5461 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 133 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflIII AluI AlwI AlwNI ApoI
AspHI AvaI AvaII BalI BanI
BanII BbsI BbvCI BbvI BclI
BcnI BmyI BpmI BpuAI BsaAI
BsaBI BsaHI BsaJI BsaWI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspHI BspLU11I
BspMI BsrBI BsrFI BsrI BssKI
BstAPI BstF5I BstNI BstUI BstXI
BstYI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraI DraII DsaI
EaeI EarI EcoO109I EcoRI EcoRII
EcoT14I FauI FbaI Fnu4HI FokI
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeII MaeIII MamI MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NaeI NciI NcoI
NdeII NgoMIV NlaIII NlaIV NspI
PflMI PleI PpuMI PstI PvuII
RcaI RsaI SacI Sau3AI Sau96I
ScrFI SexAI SfaNI SfcI SmlI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XcmI XhoII