##################################
# NO CUT ENZYMES ( 68 enzymes) #
##################################
AclI AgeI Aor51HI AscI AseI
AsnI Asp700I AvrII BbeI BciVI
BclI BglI BlnI BsaBI BsiEI
BsiWI BspDI BsrDI Bst1107I BstBI
BstZ17I ClaI CpoI DraI EagI
EclXI Eco47III Eco52I EcoRV FbaI
FseI HpaI KasI MamI MfeI
MluI MunI NaeI NarI NdeI
NgoMIV NotI NruI NspV PacI
PaeR7I PinAI PmeI PshBI Psp1406I
PvuI RsrII SalI SbfI ScaI
SfiI SfoI SfuI SgrAI SnaBI
SpeI SphI Sse8387I SspI SwaI
XbaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AatII 4660 GACGTC GACGT^C
Acc65I 4963 GGTACC G^GTACC
AccI 3190 GTAGAC GT^[AC][GT]AC
AccIII 1550 TCCGGA T^CCGGA
AcsI 2505 GAATTC [GA]^AATT[CT]
AflII 1933 CTTAAG C^TTAAG
AhdI 578 GACACTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApoI 2505 GAATTC [GA]^AATT[CT]
Asp718I 4963 GGTACC G^GTACC
AspEI 578 GACACTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 3193 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 1013 TGCGCA TGC^GCA
BamHI 276 GGATCC G^GATCC
BcgI 686 GCAGGACCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 1933 CTTAAG C^TTAAG
BseAI 1550 TCCGGA T^CCGGA
Bsp1407I 1892 TGTACA T^GTACA
BspEI 1550 TCCGGA T^CCGGA
BspHI 933 TCATGA T^CATGA
BspLU11I 2170 ACATGT A^CATGT
BsrBI 2374 GAGCGG CCGCTC(-3/-3)
BsrGI 1892 TGTACA T^GTACA
BssHII 142 GCGCGC G^CGCGC
BstEII 855 GGTCACC G^GT[ACGT]ACC
BstPI 855 GGTCACC G^GT[ACGT]ACC
DrdI 336 GACGTTGGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 578 GACACTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 855 GGTCACC G^GT[ACGT]ACC
EcoRI 2505 GAATTC G^AATTC
EcoT22I 3878 ATGCAT ATGCA^T
FspI 1013 TGCGCA TGC^GCA
HaeII 34 GGCGCT [GA]GCGC^[CT]
HincII 1495 GTTGAC GT[CT]^[GA]AC
HindII 1495 GTTGAC GT[CT]^[GA]AC
KpnI 4963 GGTACC GGTAC^C
KspI 28 CCGCGG CCGC^GG
MroI 1550 TCCGGA T^CCGGA
NheI 4233 GCTAGC G^CTAGC
NsiI 3878 ATGCAT ATGCA^T
PflFI 3193 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 1498 GACTGGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 933 TCATGA T^CATGA
SacII 28 CCGCGG CCGC^GG
SexAI 1238 ACCAGGT A^CC[AT]GGT
SspBI 1892 TGTACA T^GTACA
Tth111I 3193 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AcyI 21 GGCGTC G[GA]^CG[CT]C
4660 GACGTC
Alw44I 1656 GTGCAC G^TGCAC
2971 GTGCAC
AocI 52 CCTGAGG CC^T[ACGT]AGG
626 CCTCAGG
ApaLI 1656 GTGCAC G^TGCAC
2971 GTGCAC
BbsI 333 GAAGAC GAAGAC(2/6)
4013 GTCTTC
BbvCI 993 CCTCAGC CCTCAGC(-5/-2)
1036 GCTGAGG
BglII 2536 AGATCT A^GATCT
3763 AGATCT
BlpI 2584 GCTAAGC GC^T[ACGT]AGC
3360 GCTGAGC
Bpu1102I 2584 GCTAAGC GC^T[ACGT]AGC
3360 GCTGAGC
BpuAI 333 GAAGAC GAAGAC(2/6)
4013 GTCTTC
BsaHI 21 GGCGTC G[GA]^CG[CT]C
4660 GACGTC
BsaI 602 GGTCTC GGTCTC(1/5)
3496 GGTCTC
BsmBI 318 CGTCTC CGTCTC(1/5)
1568 GAGACG
BsrFI 2163 ACCGGC [GA]^CCGG[CT]
4546 ACCGGC
BstAPI 153 GCAGCGGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2575 GCATCCCATGC
Bsu36I 52 CCTGAGG CC^T[ACGT]AGG
626 CCTCAGG
CelII 2584 GCTAAGC GC^T[ACGT]AGC
3360 GCTGAGC
Cfr10I 2163 ACCGGC [GA]^CCGG[CT]
4546 ACCGGC
DraIII 973 CACTGGGTG CAC[ACGT][ACGT][ACGT]^GTG
4731 CACTGAGTG
Eco81I 52 CCTGAGG CC^T[ACGT]AGG
626 CCTCAGG
Hin1I 21 GGCGTC G[GA]^CG[CT]C
4660 GACGTC
HindIII 2962 AAGCTT A^AGCTT
4924 AAGCTT
SapI 1245 GCTCTTC GCTCTTC(1/4)
2391 GCTCTTC
SmaI 44 CCCGGG CCC^GGG
3465 CCCGGG
XmaCI 44 CCCGGG C^CCGGG
3465 CCCGGG
XmaI 44 CCCGGG C^CCGGG
3465 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 118 enzymes) #
#########################################
AccII AciI AfaI AflIII AluI
AlwI AlwNI ApaI AspHI AvaI
AvaII BalI BanI BanII BbrPI
BbvI BcnI BfaI BmyI BpmI
BsaAI BsaJI BsaWI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI BsoBI Bsp1286I BspMI BsrI
BssKI BssSI BstF5I BstNI BstUI
BstXI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HgaI HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NcoI NdeII NlaIII NlaIV NspI
PflMI PleI PmaCI PmlI PpuMI
PstI PvuII RsaI SacI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XcmI XhoII