################################## # NO CUT ENZYMES ( 68 enzymes) # ################################## AclI AgeI Aor51HI AscI AseI AsnI Asp700I AvrII BbeI BciVI BclI BglI BlnI BsaBI BsiEI BsiWI BspDI BsrDI Bst1107I BstBI BstZ17I ClaI CpoI DraI EagI EclXI Eco47III Eco52I EcoRV FbaI FseI HpaI KasI MamI MfeI MluI MunI NaeI NarI NdeI NgoMIV NotI NruI NspV PacI PaeR7I PinAI PmeI PshBI Psp1406I PvuI RsrII SalI SbfI ScaI SfiI SfoI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspI SwaI XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 46 enzymes) # ################################### AatII 4660 GACGTC GACGT^C Acc65I 4963 GGTACC G^GTACC AccI 3190 GTAGAC GT^[AC][GT]AC AccIII 1550 TCCGGA T^CCGGA AcsI 2505 GAATTC [GA]^AATT[CT] AflII 1933 CTTAAG C^TTAAG AhdI 578 GACACTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC ApoI 2505 GAATTC [GA]^AATT[CT] Asp718I 4963 GGTACC G^GTACC AspEI 578 GACACTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 3193 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC AviII 1013 TGCGCA TGC^GCA BamHI 276 GGATCC G^GATCC BcgI 686 GCAGGACCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BfrI 1933 CTTAAG C^TTAAG BseAI 1550 TCCGGA T^CCGGA Bsp1407I 1892 TGTACA T^GTACA BspEI 1550 TCCGGA T^CCGGA BspHI 933 TCATGA T^CATGA BspLU11I 2170 ACATGT A^CATGT BsrBI 2374 GAGCGG CCGCTC(-3/-3) BsrGI 1892 TGTACA T^GTACA BssHII 142 GCGCGC G^CGCGC BstEII 855 GGTCACC G^GT[ACGT]ACC BstPI 855 GGTCACC G^GT[ACGT]ACC DrdI 336 GACGTTGGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eam1105I 578 GACACTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoO65I 855 GGTCACC G^GT[ACGT]ACC EcoRI 2505 GAATTC G^AATTC EcoT22I 3878 ATGCAT ATGCA^T FspI 1013 TGCGCA TGC^GCA HaeII 34 GGCGCT [GA]GCGC^[CT] HincII 1495 GTTGAC GT[CT]^[GA]AC HindII 1495 GTTGAC GT[CT]^[GA]AC KpnI 4963 GGTACC GGTAC^C KspI 28 CCGCGG CCGC^GG MroI 1550 TCCGGA T^CCGGA NheI 4233 GCTAGC G^CTAGC NsiI 3878 ATGCAT ATGCA^T PflFI 3193 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC PshAI 1498 GACTGGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC RcaI 933 TCATGA T^CATGA SacII 28 CCGCGG CCGC^GG SexAI 1238 ACCAGGT A^CC[AT]GGT SspBI 1892 TGTACA T^GTACA Tth111I 3193 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### AcyI 21 GGCGTC G[GA]^CG[CT]C 4660 GACGTC Alw44I 1656 GTGCAC G^TGCAC 2971 GTGCAC AocI 52 CCTGAGG CC^T[ACGT]AGG 626 CCTCAGG ApaLI 1656 GTGCAC G^TGCAC 2971 GTGCAC BbsI 333 GAAGAC GAAGAC(2/6) 4013 GTCTTC BbvCI 993 CCTCAGC CCTCAGC(-5/-2) 1036 GCTGAGG BglII 2536 AGATCT A^GATCT 3763 AGATCT BlpI 2584 GCTAAGC GC^T[ACGT]AGC 3360 GCTGAGC Bpu1102I 2584 GCTAAGC GC^T[ACGT]AGC 3360 GCTGAGC BpuAI 333 GAAGAC GAAGAC(2/6) 4013 GTCTTC BsaHI 21 GGCGTC G[GA]^CG[CT]C 4660 GACGTC BsaI 602 GGTCTC GGTCTC(1/5) 3496 GGTCTC BsmBI 318 CGTCTC CGTCTC(1/5) 1568 GAGACG BsrFI 2163 ACCGGC [GA]^CCGG[CT] 4546 ACCGGC BstAPI 153 GCAGCGGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 2575 GCATCCCATGC Bsu36I 52 CCTGAGG CC^T[ACGT]AGG 626 CCTCAGG CelII 2584 GCTAAGC GC^T[ACGT]AGC 3360 GCTGAGC Cfr10I 2163 ACCGGC [GA]^CCGG[CT] 4546 ACCGGC DraIII 973 CACTGGGTG CAC[ACGT][ACGT][ACGT]^GTG 4731 CACTGAGTG Eco81I 52 CCTGAGG CC^T[ACGT]AGG 626 CCTCAGG Hin1I 21 GGCGTC G[GA]^CG[CT]C 4660 GACGTC HindIII 2962 AAGCTT A^AGCTT 4924 AAGCTT SapI 1245 GCTCTTC GCTCTTC(1/4) 2391 GCTCTTC SmaI 44 CCCGGG CCC^GGG 3465 CCCGGG XmaCI 44 CCCGGG C^CCGGG 3465 CCCGGG XmaI 44 CCCGGG C^CCGGG 3465 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 118 enzymes) # ######################################### AccII AciI AfaI AflIII AluI AlwI AlwNI ApaI AspHI AvaI AvaII BalI BanI BanII BbrPI BbvI BcnI BfaI BmyI BpmI BsaAI BsaJI BsaWI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I BspMI BsrI BssKI BssSI BstF5I BstNI BstUI BstXI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NcoI NdeII NlaIII NlaIV NspI PflMI PleI PmaCI PmlI PpuMI PstI PvuII RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI XhoII