##################################
# NO CUT ENZYMES ( 128 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflIII AgeI AhdI Alw44I
Aor51HI ApaI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AviII AvrII BamHI BanI
BbeI BbrPI BbsI BbvCI BcgI
BciVI BglII BlnI BlpI Bpu1102I
BpuAI BsaAI BsaI BsaWI BseAI
BsiEI BsiWI BsmBI BsmFI BsmI
BspDI BspEI BspHI BspLU11I BspMI
BsrFI BssHII Bst1107I BstAPI BstEII
BstPI BstXI BstYI BstZ17I CelII
Cfr10I ClaI CpoI DraIII EagI
Eam1105I EclXI Eco47III Eco52I EcoNI
EcoO65I EcoRI FseI FspI HaeII
HincII HindII HindIII HpaI KasI
KpnI MfeI MflI MluI MroI
MslI MunI NaeI NarI NdeI
NgoMIV NotI PacI PaeR7I PflFI
PflMI PinAI PleI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PstI
PvuI RcaI RsrII SalI SbfI
ScaI SfcI SfiI SfoI SgrAI
SnaBI SpeI SphI Sse8387I StuI
SwaI Tth111I Van91I XbaI XcmI
XhoI XhoII XmnI
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
AflII 472 CTTAAG C^TTAAG
AlwI 20 GGATC GGATC(4/5)
AlwNI 448 CAGCCCCTG CAG[ACGT][ACGT][ACGT]^CTG
BalI 1417 TGGCCA TGG^CCA
BfaI 553 CTAG C^TAG
BfrI 472 CTTAAG C^TTAAG
BglI 77 GCCGCTCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaBI 600 GATGGATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseRI 35 CTCCTC GAGGAG(10/8)
BsmAI 1472 GAGAC GTCTC(1/5)
BssSI 883 CACGAG CACGAG(-5/-1)
BstBI 322 TTCGAA TT^CGAA
CfoI 62 GCGC GCG^C
DraI 1567 TTTAAA TTT^AAA
DraII 8 AGGACCT [GA]G^G[ACGT]CC[CT]
DrdI 1165 GACGGTATGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 1417 TGGCCA [CT]^GGCC[GA]
EarI 349 CTCTTC CTCTTC(1/4)
EcoO109I 8 AGGACCT [GA]G^G[ACGT]CC[CT]
EcoRV 604 GATATC GAT^ATC
EcoT22I 875 ATGCAT ATGCA^T
FauI 59 CCCGC CCCGC(4/6)
HapII 91 CCGG C^CGG
HhaI 62 GCGC GCG^C
HinP1I 62 GCGC G^CGC
HpaII 91 CCGG C^CGG
Ksp632I 349 CTCTTC CTCTTC(1/4)
KspI 26 CCGCGG CCGC^GG
MaeI 553 CTAG C^TAG
MamI 600 GATGGATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 1417 TGGCCA TGG^CCA
MscI 1417 TGGCCA TGG^CCA
MspI 91 CCGG C^CGG
NheI 552 GCTAGC G^CTAGC
NsiI 875 ATGCAT ATGCA^T
NspI 525 ACATGC [GA]CATG^[CT]
NspV 322 TTCGAA TT^CGAA
PpuMI 8 AGGACCT [GA]G^G[AT]CC[CT]
PvuII 511 CAGCTG CAG^CTG
SacI 701 GAGCTC GAGCT^C
SacII 26 CCGCGG CCGC^GG
SapI 348 GCTCTTC GCTCTTC(1/4)
SexAI 932 ACCTGGT A^CC[AT]GGT
SfuI 322 TTCGAA TT^CGAA
SmaI 90 CCCGGG CCC^GGG
SspI 1018 AATATT AAT^ATT
XmaCI 90 CCCGGG C^CCGGG
XmaI 90 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AcyI 105 GGCGTC G[GA]^CG[CT]C
271 GGCGTC
AocI 12 CCTCAGG CC^T[ACGT]AGG
1093 CCTCAGG
BclI 182 TGATCA T^GATCA
1230 TGATCA
BcnI 90 CCCGG CC^[GC]GG
91 CCGGG
BpmI 115 CTGGAG CTGGAG(16/14)
1469 CTGGAG
BsaHI 105 GGCGTC G[GA]^CG[CT]C
271 GGCGTC
BsgI 1117 CTGCAC GTGCAG(16/14)
1078 GTGCAG
Bsp1407I 252 TGTACA T^GTACA
1311 TGTACA
BsrDI 872 GCAATG GCAATG(2/0)
990 GCAATG
BsrGI 252 TGTACA T^GTACA
1311 TGTACA
Bsu36I 12 CCTCAGG CC^T[ACGT]AGG
1093 CCTCAGG
Eco81I 12 CCTCAGG CC^T[ACGT]AGG
1093 CCTCAGG
FbaI 182 TGATCA T^GATCA
1230 TGATCA
HgaI 106 GCGTC GACGC(5/10)
272 GCGTC
Hin1I 105 GGCGTC G[GA]^CG[CT]C
271 GGCGTC
MaeII 590 ACGT A^CGT
1112 ACGT
MspA1I 26 CCGCGG C[AC]G^C[GT]G
511 CAGCTG
NciI 90 CCCGG CC^[GC]GG
91 CCGGG
NcoI 740 CCATGG C^CATGG
1277 CCATGG
NlaIV 30 GGCCCC GG[ACGT]^[ACGT]CC
622 GGAGCC
NruI 3 TCGCGA TCG^CGA
963 TCGCGA
SmlI 152 CTCAAG C^T[CT][GA]AG
472 CTTAAG
SspBI 252 TGTACA T^GTACA
1311 TGTACA
TaqI 323 TCGA T^CGA
381 TCGA
Tsp45I 1423 GTGAC ^GT[GC]AC
1464 GTCAC
TthHB8I 323 TCGA T^CGA
381 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 56 enzymes) #
#########################################
AccII AciI AfaI AluI AspHI
AvaI AvaII BanII BbvI BmyI
BsaJI BsiHKAI BsiYI BslI BsoBI
Bsp1286I BsrBI BsrI BssKI BstF5I
BstNI BstUI Cac8I Cfr13I DdeI
DpnII DsaI EcoRII EcoT14I Fnu4HI
FokI HaeIII HinfI HphI ItaI
MaeIII MboI MboII MnlI MseI
MvaI MvnI MwoI NdeII NlaIII
RsaI Sau3AI Sau96I ScrFI SfaNI
StyI TfiI Tru9I TseI Tsp509I
TspRI